FastQCFastQC Report
Thu 2 Feb 2017
SRR4062135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195891
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19160.16021527045525052No Hit
GCGCAAGACGGACCAGAGCGAAAGC16810.14056465012279548No Hit
GAATAGGACCGCGGTTCTATTTTGT15380.12860703860134412No Hit
GATTAAGAGGGACGGCCGGGGGCAT14440.12074679046836208No Hit
GGGTAGGCACACGCTGAGCCAGTCA13570.11347187996230425No Hit
ATCAGATACCGTCGTAGTTCCGACC13570.11347187996230425No Hit
GAACTACGACGGTATCTGATCGTCT13320.11138138843757499No Hit
GTATCTGATCGTCTTCGAACCTCCG13140.10987623453976993No Hit
GAATAACGCCGCCGCATCGCCAGTC13110.1096253755568024No Hit
TCGTAGTTCCGACCATAAACGATGC13020.10887279860789989No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12960.10837108064196486No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12630.10561163182932225No Hit
TATCAACGCAGAGTACTTTTTTTTT12570.10510991386338722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTAG401.2887876E-518.993181
AAGGCGT456.751228E-414.7805146
CGTCTTA653.371395E-614.6150315
TCGAACT851.7185812E-814.52723619
CCAACGA551.9620461E-413.816112519
GTATCAA14450.013.7355521
GTCTTAG1400.013.5665561
AGTATCG500.001497102913.3024638
ATAACGA1001.0053554E-813.30079212
ACCGAGT751.4750953E-512.669018
TATCGAG604.0872974E-412.6679510
CGGAATT1051.9923391E-812.6663615
CGACCAT2750.012.43762410
TAACGAA1001.4409852E-712.35021813
CCCTATA1001.4480793E-712.3455661
ATATACG1700.012.2963936
GCTCGTA1700.012.2958799
TCTCGTC853.9385322E-612.29484912
CCGACCA2950.012.239049
ACGCTAT1650.012.09213410
Produced by FastQC (version 0.11.5)