FastQCFastQC Report
Thu 2 Feb 2017
SRR4062135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195891
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16920.14148446639367634No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15360.12843979927936577No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14770.12350623928100472No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14280.11940887589253536No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13650.11414083725021762No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13060.10920727725185657No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12840.10736764471009483No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12750.10661506776119228No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12530.10477543521943053No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG12250.10243408471173375No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12120.10134702911887454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.027.085211
ATTTCGT1750.025.14424342
GATTTCG1700.024.58856241
GTATAGG1053.0595402E-923.0546741
CGCAATA3800.022.5792536
ATACGAA3900.022.00029440
TTACACG602.873307E-421.9993724
TACGAAT3950.021.72180741
CAATACG3950.021.72180738
GGTATCA6300.020.9587941
CGAATGC4100.020.92798243
TCTAGCG4100.020.92623128
CTAGCGG4150.020.6741129
GTCCTAC5450.020.1896631
AATACGA4250.020.18850339
TAGCGGC4250.020.18765830
CGCCGGT4250.020.1876587
GCAATAC4300.019.95375337
GCGCAAT4300.019.95291935
CGGTCCA4350.019.72440110
Produced by FastQC (version 0.11.5)