##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062135_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1195891
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26848099032437	32.0	32.0	32.0	32.0	32.0
2	31.419772370558857	32.0	32.0	32.0	32.0	32.0
3	31.559033390166828	32.0	32.0	32.0	32.0	32.0
4	31.638605023367514	32.0	32.0	32.0	32.0	32.0
5	31.616031895883488	32.0	32.0	32.0	32.0	32.0
6	35.24566118484042	36.0	36.0	36.0	36.0	36.0
7	35.24924512351042	36.0	36.0	36.0	36.0	36.0
8	35.20265392080047	36.0	36.0	36.0	36.0	36.0
9	35.29448336010556	36.0	36.0	36.0	36.0	36.0
10	35.16669077700225	36.0	36.0	36.0	36.0	36.0
11	35.29152071551672	36.0	36.0	36.0	36.0	36.0
12	35.20712088309052	36.0	36.0	36.0	36.0	36.0
13	35.24734612100936	36.0	36.0	36.0	36.0	36.0
14	35.210087708662414	36.0	36.0	36.0	36.0	36.0
15	35.18348076873227	36.0	36.0	36.0	36.0	36.0
16	35.19503700588097	36.0	36.0	36.0	36.0	36.0
17	35.172624428146044	36.0	36.0	36.0	36.0	36.0
18	35.17448329320983	36.0	36.0	36.0	36.0	36.0
19	35.16309429538311	36.0	36.0	36.0	36.0	36.0
20	35.16296384871197	36.0	36.0	36.0	36.0	36.0
21	35.14952951397744	36.0	36.0	36.0	36.0	36.0
22	35.13963061850955	36.0	36.0	36.0	36.0	36.0
23	35.10954677307547	36.0	36.0	36.0	36.0	36.0
24	35.09343911777913	36.0	36.0	36.0	36.0	36.0
25	35.06399997993128	36.0	36.0	36.0	36.0	36.0
26	35.0265609491166	36.0	36.0	36.0	36.0	36.0
27	35.0200436327391	36.0	36.0	36.0	36.0	36.0
28	34.993304573744595	36.0	36.0	36.0	36.0	36.0
29	34.967998755739444	36.0	36.0	36.0	36.0	36.0
30	34.93831795707134	36.0	36.0	36.0	36.0	36.0
31	34.94898531722373	36.0	36.0	36.0	36.0	36.0
32	34.91843821886777	36.0	36.0	36.0	32.0	36.0
33	34.9059972857058	36.0	36.0	36.0	32.0	36.0
34	34.8889288405047	36.0	36.0	36.0	32.0	36.0
35	34.877269751172975	36.0	36.0	36.0	32.0	36.0
36	34.849021357297616	36.0	36.0	36.0	32.0	36.0
37	34.83244375950651	36.0	36.0	36.0	32.0	36.0
38	34.80253551536051	36.0	36.0	36.0	32.0	36.0
39	34.79237656274694	36.0	36.0	36.0	32.0	36.0
40	34.78148342950988	36.0	36.0	36.0	32.0	36.0
41	34.74504616223385	36.0	36.0	36.0	32.0	36.0
42	34.7141018704882	36.0	36.0	36.0	32.0	36.0
43	34.711788114468625	36.0	36.0	36.0	32.0	36.0
44	34.661078643454964	36.0	36.0	36.0	32.0	36.0
45	34.65115215349894	36.0	36.0	36.0	32.0	36.0
46	34.62112349704112	36.0	36.0	36.0	32.0	36.0
47	34.594687977416	36.0	36.0	36.0	32.0	36.0
48	34.560900617196715	36.0	36.0	36.0	32.0	36.0
49	34.54675384295057	36.0	36.0	36.0	32.0	36.0
50	34.13043830917701	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	7.0
21	37.0
22	76.0
23	190.0
24	586.0
25	1352.0
26	2871.0
27	5636.0
28	9526.0
29	15343.0
30	23091.0
31	34209.0
32	51998.0
33	88388.0
34	200902.0
35	761675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11510572325008	17.933179259021333	11.674098535624875	26.27761648210371
2	16.062828696220095	20.146208928913392	37.34917291271367	26.441789462152844
3	18.36673941583256	23.837142212076362	28.829323767236115	28.96679460485496
4	12.19458964069468	16.00246176281952	36.07494328496493	35.72800531152087
5	14.2143389322271	37.02912723651236	33.542939950212855	15.213593881047688
6	34.5363415227642	35.880778432148084	16.385690669132888	13.197189375954832
7	30.1335991323624	30.395579530241466	20.60681115586621	18.86401018152992
8	27.926207321570278	33.70976117388625	19.10332965128093	19.260701853262546
9	27.109075994384103	13.92894502927106	18.48830704470558	40.47367193163925
10	15.773093032726226	26.995604114421802	31.504710713601824	25.726592139250148
11	37.82343039624848	21.034860200469776	22.1056099594361	19.036099443845636
12	24.2637497899056	23.640448836892325	29.287033684508035	22.808767688694036
13	29.45837036987485	19.29791260240273	25.306654201762534	25.937062825959888
14	23.36901941732148	19.76944387072066	25.16182494892929	31.69971176302857
15	24.95369561272725	27.71891418197812	22.07659393707286	25.25079626822177
16	25.652215723083742	25.78650957240872	24.026474117494576	24.534800587012963
17	23.857525476820214	26.269032880086897	25.23457405398987	24.638867589103018
18	24.64902679094799	25.227759432522586	26.355121587371404	23.768092189158025
19	25.609859092509268	24.996926977458646	25.218268220096984	24.174945709935102
20	25.609382865533558	24.69875790420794	24.81356856278462	24.87829066747388
21	26.662630624362922	24.588026835221605	24.360414118009082	24.38892842240639
22	25.79892314600578	24.582089839291374	25.224372455349194	24.394614559353652
23	24.364615474667403	24.455760981361163	25.69918638001823	25.480437163953205
24	24.708705795780478	25.11267947184895	25.49139956684255	24.687215165528027
25	24.8457213384531	24.53741487497157	25.529313781892622	25.08755000468271
26	24.293473034614532	25.74829519560827	25.898811340555323	24.059420429221873
27	24.97635638268961	24.902352487659744	25.278141758014787	24.843149371635853
28	24.317277572720872	24.972865034276186	25.8901857553555	24.819671637647446
29	24.33279481956444	24.922066019781184	25.736609905308544	25.008529255345834
30	24.41459182606878	25.199456464931536	25.962914184174767	24.42303752482492
31	25.008968268634973	25.007964825990502	24.900763703472663	25.08230320190186
32	24.93101291438243	24.932852569998897	24.810180988664378	25.325953526954297
33	24.37241248978793	24.68949877455182	25.611490698551453	25.326598037108795
34	25.057070848127154	25.09269309179334	25.737238289396448	24.112997770683062
35	25.676045184795576	24.616498688423423	25.73115063354542	23.976305493235575
36	24.112207832171574	25.526219846674604	25.180033581740098	25.18153873941372
37	25.387897838308376	25.18545091477411	25.114205652960315	24.312445593957197
38	24.685694599256955	24.891817063595262	25.468876344081526	24.953611993066257
39	25.399328696309503	24.612545384912558	24.997198720275076	24.990927198502863
40	25.683862492484682	24.877434481905123	25.376142140044532	24.062560885565656
41	24.324950350162013	25.357583359464826	25.936908121668235	24.380558168704923
42	25.629337501181197	25.59488418417138	25.24759140373016	23.52818691091727
43	24.979010289139538	24.533376148998514	25.616980425213075	24.87063313664887
44	24.412500104529908	25.435387973206897	25.162773972889124	24.989337949374075
45	24.57764669580611	25.545653793632862	25.217186976056542	24.659512534504486
46	24.3044562796909	25.255568572654223	25.197953277256673	25.242021870398197
47	24.82406202649773	25.009616381631538	25.52413460927387	24.642186982596858
48	25.601336638134324	25.56153184884884	24.395485668603385	24.441645844413447
49	24.648532751763607	25.77133806750713	24.70313853646462	24.876990644264637
50	24.95944382007439	25.83679524230018	24.594020095796207	24.609740841829225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	105.5
2	43.0
3	126.0
4	209.0
5	214.5
6	220.0
7	169.5
8	119.0
9	168.5
10	218.0
11	383.5
12	549.0
13	1027.5
14	1506.0
15	1916.5
16	2327.0
17	2404.5
18	2482.0
19	2604.5
20	2727.0
21	3193.5
22	3660.0
23	4428.5
24	5197.0
25	6726.0
26	8255.0
27	10764.0
28	13273.0
29	15497.5
30	17722.0
31	19568.5
32	21415.0
33	24820.5
34	28226.0
35	32300.5
36	36375.0
37	42042.0
38	47709.0
39	50784.5
40	53860.0
41	58192.0
42	62524.0
43	63552.0
44	64580.0
45	70647.5
46	76715.0
47	81437.5
48	86160.0
49	89408.5
50	92657.0
51	89222.0
52	85787.0
53	84009.0
54	82231.0
55	83882.5
56	85534.0
57	83287.5
58	81041.0
59	73569.0
60	66097.0
61	57868.5
62	49640.0
63	42454.0
64	35268.0
65	29727.0
66	24186.0
67	20931.5
68	17677.0
69	16327.5
70	14978.0
71	11662.5
72	8347.0
73	7563.5
74	6780.0
75	4878.0
76	2976.0
77	2705.0
78	2434.0
79	2038.5
80	1643.0
81	1327.0
82	1011.0
83	857.0
84	703.0
85	553.0
86	403.0
87	272.0
88	141.0
89	93.5
90	46.0
91	33.5
92	21.0
93	19.0
94	17.0
95	18.0
96	19.0
97	14.0
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030437556600058036
2	0.005686136947263588
3	8.361966098917042E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.017179659350226E-4
17	0.0
18	1.6723932197834084E-4
19	0.0
20	7.525769489025338E-4
21	0.0
22	0.0
23	8.361966098917042E-5
24	0.001756012880772579
25	2.508589829675113E-4
26	5.017179659350226E-4
27	6.689572879133634E-4
28	0.001421534236815897
29	5.853376269241929E-4
30	0.0013379145758267267
31	6.689572879133634E-4
32	0.0012542949148375564
33	1.6723932197834084E-4
34	7.525769489025338E-4
35	1.6723932197834084E-4
36	2.508589829675113E-4
37	0.0018396325417617493
38	0.0
39	7.525769489025338E-4
40	0.0
41	0.0013379145758267267
42	0.0058533762692419295
43	0.005602517286274418
44	0.005100799320339395
45	0.0026758291516534535
46	0.0023413505076967716
47	0.0012542949148375564
48	0.004599081354404373
49	0.00393012406649101
50	0.001588773558794238
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1195891.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.5918153189529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11831403588033	36.48596424817505
2	15.902122801310023	16.09034519886373
3	5.545344096631004	8.416470732504042
4	2.3844087290194245	4.825262642537996
5	1.2183119495507357	3.0818306576272136
6	0.6984984675209306	2.120298328163432
7	0.45474218438524855	1.6104362827109004
8	0.2991727110359185	1.210855243615972
9	0.21587960854080301	0.9829567157781737
>10	0.985054788595106	8.931350466996484
>50	0.08785557248078325	3.131594335519363
>100	0.0798380956839397	8.601212926900025
>500	0.007967207135797858	2.899624176859189
>1k	0.00248975222993683	1.6117980437484876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1692	0.14148446639367634	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1536	0.12843979927936577	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1477	0.12350623928100472	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1428	0.11940887589253536	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1365	0.11414083725021762	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1306	0.10920727725185657	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1284	0.10736764471009483	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1275	0.10661506776119228	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1253	0.10477543521943053	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1225	0.10243408471173375	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1212	0.10134702911887454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.361966098917042E-5
2	0.0	0.0	0.0	0.0	8.361966098917042E-5
3	0.0	0.0	0.0	0.0	8.361966098917042E-5
4	0.0	0.0	0.0	0.0	8.361966098917042E-5
5	0.0	0.0	0.0	0.0	8.361966098917042E-5
6	0.0	0.0	0.0	0.0	1.6723932197834084E-4
7	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
8	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
9	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
10	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
11	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
12	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
13	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
14	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
15	0.0	0.0	0.0	8.361966098917042E-5	1.6723932197834084E-4
16	0.0	0.0	0.0	1.6723932197834084E-4	1.6723932197834084E-4
17	0.0	0.0	0.0	1.6723932197834084E-4	1.6723932197834084E-4
18	0.0	0.0	0.0	1.6723932197834084E-4	1.6723932197834084E-4
19	0.0	0.0	0.0	2.508589829675113E-4	1.6723932197834084E-4
20	0.0	0.0	0.0	4.180983049458521E-4	2.508589829675113E-4
21	0.0	0.0	0.0	5.853376269241929E-4	2.508589829675113E-4
22	0.0	0.0	0.0	6.689572879133634E-4	2.508589829675113E-4
23	0.0	0.0	0.0	7.525769489025337E-4	2.508589829675113E-4
24	0.0	0.0	0.0	0.0010034359318700451	2.508589829675113E-4
25	0.0	0.0	0.0	0.0010034359318700451	2.508589829675113E-4
26	0.0	0.0	0.0	0.0012542949148375562	2.508589829675113E-4
27	0.0	0.0	0.0	0.001588773558794238	3.344786439566817E-4
28	0.0	0.0	0.0	0.0018396325417617493	3.344786439566817E-4
29	0.0	0.0	0.0	0.002843068473631794	3.344786439566817E-4
30	0.0	0.0	0.0	0.004766320676382714	3.344786439566817E-4
31	0.0	0.0	0.0	0.008278346437927872	3.344786439566817E-4
32	0.0	0.0	0.0	0.013211906436288926	3.344786439566817E-4
33	0.0	0.0	0.0	0.017392889485747447	3.344786439566817E-4
34	0.0	0.0	0.0	0.022828167450043523	3.344786439566817E-4
35	0.0	0.0	0.0	0.02809620609236126	3.344786439566817E-4
36	0.0	0.0	0.0	0.0342004413445707	3.344786439566817E-4
37	0.0	0.0	0.0	0.042980505748433595	3.344786439566817E-4
38	0.0	0.0	0.0	0.05635965150670086	3.344786439566817E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1495	0.0	27.08521	1
ATTTCGT	175	0.0	25.144243	42
GATTTCG	170	0.0	24.588562	41
GTATAGG	105	3.0595402E-9	23.054674	1
CGCAATA	380	0.0	22.57925	36
ATACGAA	390	0.0	22.000294	40
TTACACG	60	2.873307E-4	21.999372	4
TACGAAT	395	0.0	21.721807	41
CAATACG	395	0.0	21.721807	38
GGTATCA	630	0.0	20.958794	1
CGAATGC	410	0.0	20.927982	43
TCTAGCG	410	0.0	20.926231	28
CTAGCGG	415	0.0	20.67411	29
GTCCTAC	545	0.0	20.189663	1
AATACGA	425	0.0	20.188503	39
TAGCGGC	425	0.0	20.187658	30
CGCCGGT	425	0.0	20.187658	7
GCAATAC	430	0.0	19.953753	37
GCGCAAT	430	0.0	19.952919	35
CGGTCCA	435	0.0	19.724401	10
>>END_MODULE