FastQCFastQC Report
Thu 2 Feb 2017
SRR4062134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001145
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18610.1858871592027129No Hit
TATCAACGCAGAGTACTTTTTTTTT13000.12985132023832713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG601.4640045E-615.83491810
CGTCTTA601.4647685E-615.834125515
TTAGAAC551.1285745E-515.54393
GGTCGGC807.692506E-915.43904511
GTATAGA903.6550773E-813.7160461
CCCTTAC707.2901657E-613.5653211
CCTTACC500.001501156813.29733852
TTAAGAC655.453809E-513.1525313
TCGGCGT957.3714546E-813.0006513
CTAAGGC957.3860065E-812.99873
GGTATCA5100.012.8471571
GCGGTCG751.4750558E-512.6685689
GTATCAA11350.012.3820911
TTATACT853.941541E-612.2935034
TTCGAAG853.94816E-612.2916582
ATAAACC701.09116845E-412.2130643
GTCCTAA701.0947406E-412.2087891
ACGGTAT1650.012.0927249
GTTTAAG550.00307748712.0854681
GTCCTAT1501.4551915E-1112.0279181
Produced by FastQC (version 0.11.5)