##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062134_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001145
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192550529643558	32.0	32.0	32.0	32.0	32.0
2	30.68112511174705	32.0	32.0	32.0	32.0	32.0
3	30.698526187515295	32.0	32.0	32.0	32.0	32.0
4	30.705898745935905	32.0	32.0	32.0	32.0	32.0
5	30.614035928861455	32.0	32.0	32.0	32.0	32.0
6	34.25145708164152	36.0	36.0	36.0	32.0	36.0
7	34.1858641855076	36.0	36.0	36.0	32.0	36.0
8	34.13361501081262	36.0	36.0	36.0	32.0	36.0
9	34.29154817733695	36.0	36.0	36.0	32.0	36.0
10	33.94038326116596	36.0	36.0	36.0	32.0	36.0
11	34.28711924846051	36.0	36.0	36.0	32.0	36.0
12	34.072633834259776	36.0	36.0	36.0	32.0	36.0
13	34.17519839783448	36.0	36.0	36.0	32.0	36.0
14	34.069365576415	36.0	36.0	36.0	32.0	36.0
15	34.01888837281313	36.0	36.0	36.0	32.0	36.0
16	34.027692292325284	36.0	36.0	36.0	32.0	36.0
17	33.95271114573813	36.0	36.0	36.0	32.0	36.0
18	33.965182865618864	36.0	36.0	36.0	32.0	36.0
19	33.96530572494494	36.0	36.0	36.0	32.0	36.0
20	33.94875068047086	36.0	36.0	36.0	32.0	36.0
21	33.928975323254875	36.0	36.0	36.0	32.0	36.0
22	33.89324523420683	36.0	36.0	36.0	32.0	36.0
23	33.8733709902162	36.0	36.0	36.0	32.0	36.0
24	33.84037676859995	36.0	36.0	36.0	32.0	36.0
25	33.47608188624025	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	9.0
5	68.0
6	202.0
7	47.0
8	179.0
9	124.0
10	77.0
11	14.0
12	40.0
13	40.0
14	123.0
15	225.0
16	348.0
17	487.0
18	652.0
19	982.0
20	1382.0
21	2003.0
22	3021.0
23	4557.0
24	6607.0
25	9370.0
26	12902.0
27	16462.0
28	21703.0
29	29184.0
30	38061.0
31	51776.0
32	72731.0
33	101338.0
34	212441.0
35	413988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58683852511252	17.662470758160246	12.052132881312591	25.698557835414636
2	16.573872637448034	19.553170279540765	37.43554860349481	26.437408479516396
3	18.896358151707055	22.9714125896478	28.44547216173562	29.68675709690952
4	12.860480750884811	15.776692482660861	35.2549649920734	36.10786177438093
5	15.038767435354302	36.25444626513728	33.25826305903041	15.448523240477998
6	34.759095329426785	34.91850908658416	16.472188545996616	13.850207037992446
7	30.643625935421426	30.293855316153444	20.231600545554173	18.830918202870958
8	28.243796983835264	32.88693481908411	19.107409622049477	19.761858575031148
9	27.097170971709716	14.630546305463055	18.826188261882617	39.44609446094461
10	16.653747945923403	26.73835139787226	30.74888907336267	25.859011582841674
11	36.82155849377809	21.468173275333502	21.96698650593685	19.743281724951565
12	24.308343484896675	23.816167689824233	28.508590322909726	23.366898502369366
13	29.16813348588253	19.61313976653572	25.10471088993255	26.114015857649196
14	23.5243765612219	19.705045309184435	25.099648206332287	31.670929923261383
15	25.208637554759854	27.142385629413297	22.02466443959913	25.62431237622772
16	25.78337663480558	25.952382381000955	23.45089733050924	24.813343653684225
17	24.06811426651252	26.08980026162407	25.05330490405117	24.78878056781224
18	24.705890587798844	25.35045642580943	25.73023629747623	24.213416688915487
19	25.354526258538222	25.322832850767043	25.31373474317342	24.008906147521316
20	25.6177467987939	24.701973910470205	24.852536570930408	24.82774271980549
21	26.540187229822408	24.423054621361477	24.56121759368489	24.475540555131225
22	25.77737782777153	24.302062242219723	25.071766029246344	24.848793900762406
23	24.44344256887117	24.445441877110518	25.37402058887625	25.73709496514206
24	24.49250339664065	25.120144603415334	25.300698923172437	25.086653076771576
25	24.855013421443317	24.64926794399484	25.188525040864175	25.30719359369767
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	255.0
1	255.0
2	292.5
3	330.0
4	330.0
5	330.0
6	735.0
7	1140.0
8	1140.0
9	1140.0
10	1396.5
11	1653.0
12	1653.0
13	1653.0
14	2387.5
15	3122.0
16	3122.0
17	3122.0
18	5121.5
19	7121.0
20	7121.0
21	7121.0
22	11154.0
23	15187.0
24	15187.0
25	15187.0
26	21506.0
27	27825.0
28	27825.0
29	27825.0
30	34577.0
31	41329.0
32	41329.0
33	41329.0
34	51262.0
35	61195.0
36	61195.0
37	61195.0
38	71126.5
39	81058.0
40	81058.0
41	81058.0
42	93208.0
43	105358.0
44	105358.0
45	105358.0
46	116465.0
47	127572.0
48	127572.0
49	127572.0
50	131865.5
51	136159.0
52	136159.0
53	136159.0
54	127334.5
55	118510.0
56	118510.0
57	118510.0
58	109121.5
59	99733.0
60	99733.0
61	99733.0
62	87742.0
63	75751.0
64	75751.0
65	75751.0
66	61766.5
67	47782.0
68	47782.0
69	47782.0
70	36264.5
71	24747.0
72	24747.0
73	24747.0
74	18749.5
75	12752.0
76	12752.0
77	12752.0
78	10102.5
79	7453.0
80	7453.0
81	7453.0
82	5133.0
83	2813.0
84	2813.0
85	2813.0
86	2085.0
87	1357.0
88	1357.0
89	1357.0
90	895.0
91	433.0
92	433.0
93	433.0
94	282.5
95	132.0
96	132.0
97	132.0
98	255.0
99	378.0
100	378.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010987419404781525
2	2.9965689285767796E-4
3	4.994281547627967E-4
4	0.008590164261920103
5	0.03046511744053059
6	0.060330921095345824
7	0.10597865444066544
8	0.1065779682263808
9	0.11536790375020602
10	0.13025086276213735
11	0.1370430856669114
12	0.12975143460737457
13	0.12475715305974659
14	0.11257110608353435
15	0.1323484610121411
16	0.11776515889306745
17	0.12355852548831589
18	0.10907510900019478
19	0.09359283620254809
20	0.0895974109644457
21	0.08759969834539452
22	0.10188334357161051
23	0.0798086191310949
24	0.08909798280968291
25	0.0878993552382522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1001145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.91827943995417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32919480617402	46.73939667336722
2	12.753628674722908	15.028437162614733
3	3.649154217411399	6.450056637027961
4	1.4981960312383955	3.530845296973364
5	0.7405538165700902	2.1816078352500567
6	0.4204853729331293	1.4864564821732473
7	0.2867076109469636	1.1824623397534406
8	0.20826563858378738	0.9816522473456069
9	0.15619313121736816	0.8282367496499694
>10	0.8133884456848129	8.685935062265468
>50	0.07637573447893321	3.1920040635773756
>100	0.06224432707423684	7.26261306757683
>500	0.005272060057233833	2.1335634608232135
>1k	3.401329069183118E-4	0.3167329216015675
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1861	0.1858871592027129	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1300	0.12985132023832713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	9.988563095255932E-5	0.0	0.0	1.9977126190511864E-4
13	0.0	9.988563095255932E-5	0.0	0.0	1.9977126190511864E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGG	60	1.4640045E-6	15.834918	10
CGTCTTA	60	1.4647685E-6	15.8341255	15
TTAGAAC	55	1.1285745E-5	15.5439	3
GGTCGGC	80	7.692506E-9	15.439045	11
GTATAGA	90	3.6550773E-8	13.716046	1
CCCTTAC	70	7.2901657E-6	13.565321	1
CCTTACC	50	0.0015011568	13.2973385	2
TTAAGAC	65	5.453809E-5	13.152531	3
TCGGCGT	95	7.3714546E-8	13.00065	13
CTAAGGC	95	7.3860065E-8	12.9987	3
GGTATCA	510	0.0	12.847157	1
GCGGTCG	75	1.4750558E-5	12.668568	9
GTATCAA	1135	0.0	12.382091	1
TTATACT	85	3.941541E-6	12.293503	4
TTCGAAG	85	3.94816E-6	12.291658	2
ATAAACC	70	1.09116845E-4	12.213064	3
GTCCTAA	70	1.0947406E-4	12.208789	1
ACGGTAT	165	0.0	12.092724	9
GTTTAAG	55	0.003077487	12.085468	1
GTCCTAT	150	1.4551915E-11	12.027918	1
>>END_MODULE