FastQCFastQC Report
Thu 2 Feb 2017
SRR4062134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001145
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10290.10278231425018354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.025.3072681
GTATCAA13900.025.1726971
TTCGGGT500.002580317721.99972538
CGGATCG1158.767529E-921.04321526
TCGATTT1057.743802E-820.9521230
GTATATA1502.1827873E-1120.5392321
TAGACCG550.004483406419.999755
CGTCGTA3000.019.79975310
ACCGTCG2850.019.2980048
TACCGTC3200.019.249767
CGATTTT1152.0208063E-719.13019631
ATACCGT2950.018.6438356
ATCGATT1203.1610398E-718.33310529
GTAAACG2050.018.24367527
GTACTAA851.4273782E-418.1228521
TCTATAC1102.795785E-617.9997753
CGAACGT1355.5235432E-817.92570326
GATAATT1355.5235432E-817.9257037
CCGTCGT3100.017.7417149
TCAACGC21400.016.75684
Produced by FastQC (version 0.11.5)