##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001145
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265051516014164	32.0	32.0	32.0	32.0	32.0
2	31.42221356546754	32.0	32.0	32.0	32.0	32.0
3	31.550463719041698	32.0	32.0	32.0	32.0	32.0
4	31.626979108920285	32.0	32.0	32.0	32.0	32.0
5	31.605968166449415	32.0	32.0	32.0	32.0	32.0
6	35.23391117170839	36.0	36.0	36.0	36.0	36.0
7	35.23828516348781	36.0	36.0	36.0	36.0	36.0
8	35.18761418176188	36.0	36.0	36.0	36.0	36.0
9	35.27740037656883	36.0	36.0	36.0	36.0	36.0
10	35.14972456537265	36.0	36.0	36.0	36.0	36.0
11	35.2777120197374	36.0	36.0	36.0	36.0	36.0
12	35.19297604243142	36.0	36.0	36.0	36.0	36.0
13	35.22421127808659	36.0	36.0	36.0	36.0	36.0
14	35.19385703369642	36.0	36.0	36.0	36.0	36.0
15	35.16953488255947	36.0	36.0	36.0	36.0	36.0
16	35.180956804458894	36.0	36.0	36.0	36.0	36.0
17	35.163732526257434	36.0	36.0	36.0	36.0	36.0
18	35.16217830583982	36.0	36.0	36.0	36.0	36.0
19	35.15152650215503	36.0	36.0	36.0	36.0	36.0
20	35.150047195960624	36.0	36.0	36.0	36.0	36.0
21	35.14296230815716	36.0	36.0	36.0	36.0	36.0
22	35.12788257445225	36.0	36.0	36.0	36.0	36.0
23	35.09370370925291	36.0	36.0	36.0	36.0	36.0
24	35.07238711675132	36.0	36.0	36.0	36.0	36.0
25	35.06546803909524	36.0	36.0	36.0	36.0	36.0
26	35.02601421372528	36.0	36.0	36.0	36.0	36.0
27	35.0076971867212	36.0	36.0	36.0	36.0	36.0
28	34.991057239460815	36.0	36.0	36.0	36.0	36.0
29	34.972240784301974	36.0	36.0	36.0	36.0	36.0
30	34.949800478452175	36.0	36.0	36.0	36.0	36.0
31	34.95405660518706	36.0	36.0	36.0	36.0	36.0
32	34.92341668789236	36.0	36.0	36.0	32.0	36.0
33	34.906942550779355	36.0	36.0	36.0	32.0	36.0
34	34.890841985926116	36.0	36.0	36.0	32.0	36.0
35	34.88189922538693	36.0	36.0	36.0	32.0	36.0
36	34.85580909858212	36.0	36.0	36.0	32.0	36.0
37	34.85136618571735	36.0	36.0	36.0	32.0	36.0
38	34.8300635772041	36.0	36.0	36.0	32.0	36.0
39	34.81079464013704	36.0	36.0	36.0	32.0	36.0
40	34.80397744582453	36.0	36.0	36.0	32.0	36.0
41	34.766100814567324	36.0	36.0	36.0	32.0	36.0
42	34.74665308222086	36.0	36.0	36.0	32.0	36.0
43	34.74281847284859	36.0	36.0	36.0	32.0	36.0
44	34.68797227174885	36.0	36.0	36.0	32.0	36.0
45	34.68483786064956	36.0	36.0	36.0	32.0	36.0
46	34.654187954791766	36.0	36.0	36.0	32.0	36.0
47	34.633193992878155	36.0	36.0	36.0	32.0	36.0
48	34.60784501745501	36.0	36.0	36.0	32.0	36.0
49	34.59442738064916	36.0	36.0	36.0	32.0	36.0
50	34.17044284294483	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	24.0
22	58.0
23	196.0
24	470.0
25	1031.0
26	2260.0
27	4581.0
28	7758.0
29	12754.0
30	19145.0
31	28756.0
32	43795.0
33	73926.0
34	169527.0
35	636852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1678706673461	18.11536424767443	12.54608674799916	26.17067833698031
2	15.951142085711117	19.8942770353219	37.11471401158917	27.039866867377814
3	18.13776853056956	23.47456856812663	28.774310962569782	29.613351938734027
4	12.562216262379575	16.118843923707356	35.60013784217072	35.71880197174235
5	14.702680133926787	36.56167344557826	33.09733664687597	15.63830977361898
6	34.805417407902766	35.08689567823977	16.47167287790056	13.6360140359569
7	30.61304805997133	30.379515454804253	20.228837980512314	18.778598504712104
8	28.209799779252755	33.021190736606584	19.111017884522223	19.657991599618438
9	27.164596536965174	14.244989487037344	18.838429997652685	39.751983978344796
10	16.526377297993797	26.73089312736916	30.905712958662328	25.83701661597471
11	37.12409291361391	21.30900119363329	21.917804114289137	19.64910177846366
12	24.28449425407908	23.70725519280424	28.617433039170148	23.390817513946534
13	29.225436874778378	19.572189842630188	25.19265441069975	26.009718871891685
14	23.59648202807785	19.504467384844354	25.17577373906877	31.72327684800903
15	25.183065390128302	27.057319369322126	22.10748692746805	25.652128313081523
16	25.800316640612902	25.916584676392297	23.53318982954347	24.749908853451334
17	24.15773938840028	26.005723446653583	25.054512583092357	24.78202458185378
18	24.81750876996716	25.237827924853967	25.80427990379006	24.140383401388814
19	25.538158808164653	25.135419944163935	25.380838939414367	23.945582308257045
20	25.788224059899534	24.3998110160087	24.846999879138146	24.96496504495362
21	26.58890887715341	24.38432871228186	24.66520766763589	24.36155474292883
22	25.936302933141555	24.287690594269563	25.07089382656858	24.705112646020307
23	24.636016397241555	24.3866017276236	25.33651502680933	25.640866848325516
24	24.598107353831338	25.0177298234609	25.316789493556335	25.067373329151426
25	24.969135309206266	24.449130195056856	25.319933995509135	25.26180050022774
26	24.729409555468543	25.28747113302382	25.497530824736504	24.485588486771135
27	25.28342632713441	24.778602835561788	24.99175941075292	24.946211426550878
28	24.802097640154827	24.669546759895116	25.52038956174304	25.00796603820702
29	24.614339652795813	24.892822182711708	25.516810835647362	24.976027328845117
30	24.724487156052195	24.943439083518374	25.611382303849737	24.720691456579697
31	25.122185830339244	24.730032492990482	24.827022021917035	25.32075965475324
32	25.125732042667487	24.897691324963517	24.693223804534245	25.283352827834754
33	24.68029042804075	24.680590085532238	25.13786741754175	25.501252068885265
34	25.225543782263347	24.722814882293715	25.451986543286825	24.599654792156112
35	25.675704021711226	24.53890111382912	25.42868586942299	24.356708995036662
36	24.663359107258618	24.984093149716173	25.019053260230077	25.333494482795132
37	25.45276382963634	24.934648781525656	25.024147737204693	24.588439651633305
38	24.9390447937112	24.605227015067747	25.345679197319072	25.110048993901984
39	25.309147571768186	24.662484767365203	24.87594142677348	25.152426234093134
40	25.79945961873655	24.72908519744892	25.081581589080503	24.38987359473403
41	24.599556505583635	25.220948119144175	25.445392252831773	24.734103122440416
42	25.674606583544936	25.09854140736908	25.02512231520864	24.201729693877347
43	25.339398606944897	24.323325432095803	25.15150697876056	25.18576898219874
44	24.851164314596687	24.964439259058	25.049745081430103	25.134651344915216
45	25.14106601859704	25.09222106685765	24.787963964611883	24.978748949933426
46	24.767687745160224	25.105605560111517	24.97355490939747	25.15315178533079
47	24.935223456600212	24.933225722031217	25.310597782115078	24.82095303925349
48	25.761124823572068	25.258985712958626	24.341810198008822	24.63807926546048
49	24.971956044658565	25.501369983428347	24.531743360100368	24.994930611812723
50	25.27094383346818	25.479008720146233	24.516196697731562	24.73385074865402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	73.5
2	27.0
3	71.0
4	115.0
5	121.0
6	127.0
7	114.5
8	102.0
9	140.5
10	179.0
11	302.5
12	426.0
13	797.5
14	1169.0
15	1481.5
16	1794.0
17	1878.5
18	1963.0
19	2039.0
20	2115.0
21	2594.5
22	3074.0
23	3757.0
24	4440.0
25	5566.0
26	6692.0
27	8337.5
28	9983.0
29	11477.0
30	12971.0
31	14487.0
32	16003.0
33	18176.0
34	20349.0
35	23385.0
36	26421.0
37	31119.0
38	35817.0
39	39784.0
40	43751.0
41	47577.5
42	51404.0
43	53647.0
44	55890.0
45	60220.5
46	64551.0
47	68524.5
48	72498.0
49	75605.5
50	78713.0
51	76391.0
52	74069.0
53	73742.5
54	73416.0
55	73828.5
56	74241.0
57	71707.0
58	69173.0
59	63235.0
60	57297.0
61	50166.0
62	43035.0
63	37348.0
64	31661.0
65	26522.0
66	21383.0
67	18242.5
68	15102.0
69	13401.0
70	11700.0
71	9188.0
72	6676.0
73	5923.5
74	5171.0
75	3789.0
76	2407.0
77	2128.5
78	1850.0
79	1666.5
80	1483.0
81	1147.5
82	812.0
83	655.5
84	499.0
85	401.5
86	304.0
87	200.5
88	97.0
89	60.0
90	23.0
91	18.5
92	14.0
93	14.5
94	15.0
95	13.5
96	12.0
97	9.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03146397375005618
2	0.0037956539761972536
3	1.9977126190511864E-4
4	0.0
5	9.988563095255932E-5
6	1.9977126190511864E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.988563095255933E-4
17	0.0
18	9.988563095255932E-5
19	0.0
20	1.9977126190511864E-4
21	1.9977126190511864E-4
22	0.0
23	9.988563095255932E-5
24	6.991994166679152E-4
25	9.988563095255932E-5
26	9.988563095255932E-5
27	2.9965689285767796E-4
28	0.0019977126190511866
29	4.994281547627967E-4
30	0.0011986275714307118
31	5.993137857153559E-4
32	7.990850476204746E-4
33	1.9977126190511864E-4
34	8.989706785730338E-4
35	3.995425238102373E-4
36	3.995425238102373E-4
37	0.0015981700952409491
38	0.0
39	4.994281547627967E-4
40	0.0
41	4.994281547627967E-4
42	0.004295082130960051
43	0.0035958827142921353
44	0.004095310869054933
45	0.0017979413571460677
46	0.0013983988333358304
47	0.0010987419404781525
48	0.0027967976666716608
49	0.0037956539761972536
50	0.0014982844642883898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1001145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.11524691635452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86961481634457	47.41266368882327
2	13.262681263731457	15.94578717884251
3	3.732120086686576	6.730719615979498
4	1.4878389222214339	3.5776721672441716
5	0.6786068994833712	2.0397310660792316
6	0.4465284074457068	1.6105899281259135
7	0.2804009140427574	1.1799459128276304
8	0.20305468407109512	0.9765345976364995
9	0.14737865963294156	0.7973734062632089
>10	0.7636183667042	8.339971534209873
>50	0.06825836462736809	2.8634931974121436
>100	0.055226857184266794	6.593010941452353
>500	0.004504909330529104	1.8292966409904021
>1k	1.6684849372330016E-4	0.10321012411334159
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1029	0.10278231425018354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.9977126190511864E-4
11	0.0	0.0	0.0	0.0	1.9977126190511864E-4
12	0.0	0.0	0.0	0.0	3.995425238102373E-4
13	0.0	0.0	0.0	0.0	4.994281547627965E-4
14	0.0	0.0	0.0	0.0	6.991994166679152E-4
15	0.0	0.0	0.0	0.0	9.98856309525593E-4
16	0.0	0.0	0.0	9.988563095255932E-5	0.0010987419404781525
17	0.0	0.0	0.0	9.988563095255932E-5	0.0010987419404781525
18	0.0	0.0	0.0	9.988563095255932E-5	0.0012985132023832712
19	0.0	0.0	0.0	9.988563095255932E-5	0.0012985132023832712
20	0.0	0.0	0.0	2.9965689285767796E-4	0.0012985132023832712
21	0.0	0.0	0.0	3.995425238102373E-4	0.0012985132023832712
22	0.0	0.0	0.0	3.995425238102373E-4	0.0012985132023832712
23	0.0	0.0	0.0	5.993137857153559E-4	0.0012985132023832712
24	9.988563095255932E-5	0.0	0.0	7.990850476204746E-4	0.0012985132023832712
25	9.988563095255932E-5	0.0	0.0	9.98856309525593E-4	0.0012985132023832712
26	9.988563095255932E-5	0.0	0.0	0.0013983988333358304	0.0014982844642883898
27	9.988563095255932E-5	0.0	0.0	0.001897826988098627	0.0015981700952409491
28	9.988563095255932E-5	0.0	0.0	0.002497140773813983	0.0017979413571460677
29	9.988563095255932E-5	0.0	0.0	0.003396111452387017	0.0017979413571460677
30	9.988563095255932E-5	0.0	0.0	0.004594739023817729	0.0017979413571460677
31	9.988563095255932E-5	0.0	0.0	0.008090736107157305	0.001897826988098627
32	9.988563095255932E-5	0.0	0.0	0.010687762511923847	0.001897826988098627
33	9.988563095255932E-5	0.0	0.0	0.013684331440500627	0.001897826988098627
34	9.988563095255932E-5	0.0	0.0	0.01897826988098627	0.001897826988098627
35	9.988563095255932E-5	0.0	0.0	0.02337323764289888	0.001897826988098627
36	9.988563095255932E-5	0.0	0.0	0.030165460547672916	0.001897826988098627
37	9.988563095255932E-5	0.0	0.0	0.03965459548816605	0.001897826988098627
38	9.988563095255932E-5	0.0	0.0	0.05413801197628715	0.001897826988098627
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	600	0.0	25.307268	1
GTATCAA	1390	0.0	25.172697	1
TTCGGGT	50	0.0025803177	21.999725	38
CGGATCG	115	8.767529E-9	21.043215	26
TCGATTT	105	7.743802E-8	20.95212	30
GTATATA	150	2.1827873E-11	20.539232	1
TAGACCG	55	0.0044834064	19.99975	5
CGTCGTA	300	0.0	19.799753	10
ACCGTCG	285	0.0	19.298004	8
TACCGTC	320	0.0	19.24976	7
CGATTTT	115	2.0208063E-7	19.130196	31
ATACCGT	295	0.0	18.643835	6
ATCGATT	120	3.1610398E-7	18.333105	29
GTAAACG	205	0.0	18.243675	27
GTACTAA	85	1.4273782E-4	18.122852	1
TCTATAC	110	2.795785E-6	17.999775	3
CGAACGT	135	5.5235432E-8	17.925703	26
GATAATT	135	5.5235432E-8	17.925703	7
CCGTCGT	310	0.0	17.741714	9
TCAACGC	2140	0.0	16.7568	4
>>END_MODULE