FastQCFastQC Report
Thu 2 Feb 2017
SRR4062133_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062133_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337537
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC49090.14708451172226705No Hit
GTATCAACGCAGAGTACTTTTTTTT41780.1251821328123104No Hit
GAATAGGACCGCGGTTCTATTTTGT41700.12494243509510157No Hit
GGGTAGGCACACGCTGAGCCAGTCA40010.11987882081906508No Hit
TCGTAGTTCCGACCATAAACGATGC38200.11445565996721534No Hit
GTCTTGCGCCGGTCCAAGAATTTCA38170.11436577332326205No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG38120.11421596225000652No Hit
GAACTACGACGGTATCTGATCGTCT37580.11259800265884692No Hit
GATTAAGAGGGACGGCCGGGGGCAT37280.11169913621931382No Hit
ATCAGATACCGTCGTAGTTCCGACC35410.10609620207955746No Hit
GTATCTGATCGTCTTCGAACCTCCG34580.10360933826351588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.015.2666721
GTATCAA33000.013.4082691
AACCGCG2300.013.2193347
CGGTCCA7600.012.62742310
GCGTTAT4100.012.2741861
CGTTATT4200.011.9848212
CAAGACG9050.011.8609514
CGCCGGT7700.011.7225017
TCGCGTA3900.011.6945529
AAGACGG9100.011.6927975
CGCGTAA3800.011.50220810
CGCATCG6050.011.46363413
TATGCCG752.0743665E-411.4004775
TAGATCG1251.8333594E-811.4004775
CTATTAG1251.848457E-811.3941511
GCCGGTC8350.011.3787468
CGGTTCT7950.011.35369912
ATCGCCA6150.011.27621816
CCGGTCC8950.011.2535569
CGCAAGA8700.011.2440552
Produced by FastQC (version 0.11.5)