##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062133_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3337537
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22425159631189	32.0	32.0	32.0	32.0	32.0
2	30.858400970536056	32.0	32.0	32.0	32.0	32.0
3	30.88032522186271	32.0	32.0	32.0	32.0	32.0
4	30.88472127799632	32.0	32.0	32.0	32.0	32.0
5	30.839736008919154	32.0	32.0	32.0	32.0	32.0
6	34.40274100332071	36.0	36.0	36.0	32.0	36.0
7	34.36316451323236	36.0	36.0	36.0	32.0	36.0
8	34.34134782625631	36.0	36.0	36.0	32.0	36.0
9	34.439359923200854	36.0	36.0	36.0	32.0	36.0
10	34.215085555605825	36.0	36.0	36.0	32.0	36.0
11	34.38893741103095	36.0	36.0	36.0	32.0	36.0
12	34.280118542506045	36.0	36.0	36.0	32.0	36.0
13	34.346470765717356	36.0	36.0	36.0	32.0	36.0
14	34.26941214434477	36.0	36.0	36.0	32.0	36.0
15	34.23609955485138	36.0	36.0	36.0	32.0	36.0
16	34.227045872450255	36.0	36.0	36.0	32.0	36.0
17	34.17577123489567	36.0	36.0	36.0	32.0	36.0
18	34.17142102095048	36.0	36.0	36.0	32.0	36.0
19	34.17009968728436	36.0	36.0	36.0	32.0	36.0
20	34.16427533237834	36.0	36.0	36.0	32.0	36.0
21	34.137756675057084	36.0	36.0	36.0	32.0	36.0
22	34.1066331249661	36.0	36.0	36.0	32.0	36.0
23	34.09248167136424	36.0	36.0	36.0	32.0	36.0
24	34.06032262713492	36.0	36.0	36.0	32.0	36.0
25	33.70350650794283	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	50.0
5	252.0
6	700.0
7	153.0
8	504.0
9	405.0
10	237.0
11	74.0
12	133.0
13	132.0
14	432.0
15	571.0
16	1069.0
17	1480.0
18	2157.0
19	2932.0
20	4144.0
21	5868.0
22	8469.0
23	12394.0
24	17824.0
25	25343.0
26	35259.0
27	46870.0
28	63380.0
29	85281.0
30	113623.0
31	156221.0
32	224440.0
33	323958.0
34	715826.0
35	1487355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14369266862522	17.12042378962728	11.673810226501534	26.06207331524597
2	17.111211850916142	18.90424388567477	36.93595654991669	27.0485877134924
3	19.295068547985988	22.345540815384854	27.71522293825707	30.644167698372083
4	12.92438627676368	14.959312855315817	34.6779848326667	37.4383160352538
5	15.477091068651863	35.84501149133145	32.83454640711563	15.843351032901055
6	35.866944206016306	34.26778509867927	15.986074664001201	13.879196031303225
7	31.55362562433585	29.63709115055953	19.69222675812045	19.11705646698417
8	28.627533904113182	32.70247612877489	18.659492176896762	20.010497790215158
9	26.89218881468139	14.547243415438194	18.392978467159306	40.16758930272111
10	16.500720753964146	26.127083698960345	30.293716615441387	27.078478931634127
11	37.84772976566794	21.017789122748905	21.510281280077574	19.624199831505575
12	24.891204107873683	23.271880829422802	27.87760859639049	23.959306466313024
13	29.086451277691467	19.026762778639576	25.084498959493594	26.80228698417536
14	23.599655056053393	19.52316748528364	24.298961418769448	32.57821603989351
15	25.3066684506722	27.08714397014631	21.54948680340909	26.056700775772406
16	26.280938734152308	25.7195616009042	22.979987197661742	25.019512467281746
17	24.39349457216076	25.996333744994597	24.667128823177737	24.943042859666907
18	25.300151410444684	24.813946317678788	25.447092727499594	24.438809544376937
19	25.78523911803463	24.91898243254834	24.708753616025614	24.587024833391414
20	25.950179809946043	24.231729318257607	24.308799291795914	25.509291580000433
21	26.799914233749018	24.04972643264875	24.04414862751507	25.106210706087158
22	26.126307158129432	24.09828695440666	24.582985552616684	25.19242033484722
23	24.516861641168155	24.148042288886902	25.248983048103042	26.0861130218419
24	24.90687802603307	24.761943318623945	24.856467257741173	25.474711397601812
25	24.99228569295169	24.33945589920332	24.80033514128502	25.867923266559973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	175.0
1	175.0
2	508.5
3	842.0
4	842.0
5	842.0
6	1929.5
7	3017.0
8	3017.0
9	3017.0
10	3637.0
11	4257.0
12	4257.0
13	4257.0
14	6084.0
15	7911.0
16	7911.0
17	7911.0
18	13126.5
19	18342.0
20	18342.0
21	18342.0
22	29848.5
23	41355.0
24	41355.0
25	41355.0
26	62163.5
27	82972.0
28	82972.0
29	82972.0
30	103776.5
31	124581.0
32	124581.0
33	124581.0
34	158772.0
35	192963.0
36	192963.0
37	192963.0
38	226365.0
39	259767.0
40	259767.0
41	259767.0
42	298517.0
43	337267.0
44	337267.0
45	337267.0
46	379262.5
47	421258.0
48	421258.0
49	421258.0
50	437503.0
51	453748.0
52	453748.0
53	453748.0
54	427563.5
55	401379.0
56	401379.0
57	401379.0
58	373673.5
59	345968.0
60	345968.0
61	345968.0
62	307646.5
63	269325.0
64	269325.0
65	269325.0
66	223014.0
67	176703.0
68	176703.0
69	176703.0
70	134276.0
71	91849.0
72	91849.0
73	91849.0
74	70786.5
75	49724.0
76	49724.0
77	49724.0
78	40767.5
79	31811.0
80	31811.0
81	31811.0
82	22294.0
83	12777.0
84	12777.0
85	12777.0
86	9390.0
87	6003.0
88	6003.0
89	6003.0
90	3934.0
91	1865.0
92	1865.0
93	1865.0
94	1128.5
95	392.0
96	392.0
97	392.0
98	839.0
99	1286.0
100	1286.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.587908688353117E-4
2	1.7977328790662095E-4
3	6.292065076731733E-4
4	0.010936208347652774
5	0.03322809604807377
6	0.0609431446003445
7	0.10169175652584526
8	0.10492767570816444
9	0.11361671795698444
10	0.12650047025695893
11	0.1345003815688036
12	0.12677013018881889
13	0.1217664403420846
14	0.10972163005234101
15	0.12626077253975013
16	0.11298751144931127
17	0.1165530149927926
18	0.1064557486553707
19	0.09213381005214324
20	0.08704023356145565
21	0.0866507247709913
22	0.09887530834864153
23	0.07703285386798708
24	0.08784921335703545
25	0.08461329417471626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3337537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.57367752885531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8786083893991	34.873721609373135
2	14.131395017818152	13.163020691854705
3	4.6465622589324465	6.492224767958077
4	1.9990565636936721	3.7241366303764276
5	1.0686567621282128	2.4885637714195012
6	0.6801332296346367	1.900578342819748
7	0.4748439210374745	1.5480659358455604
8	0.31433914028261356	1.1711943803376046
9	0.25093785765560284	1.0518388976010458
>10	1.3582719756308195	11.649656590161472
>50	0.09996755734198216	3.241601321932707
>100	0.076122098725297	7.428852312773332
>500	0.012494812730628243	4.03271029207715
>1k	0.00861041498937815	7.23383445546961
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	4909	0.14708451172226705	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4178	0.1251821328123104	No Hit
GAATAGGACCGCGGTTCTATTTTGT	4170	0.12494243509510157	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4001	0.11987882081906508	No Hit
TCGTAGTTCCGACCATAAACGATGC	3820	0.11445565996721534	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3817	0.11436577332326205	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3812	0.11421596225000652	No Hit
GAACTACGACGGTATCTGATCGTCT	3758	0.11259800265884692	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3728	0.11169913621931382	No Hit
ATCAGATACCGTCGTAGTTCCGACC	3541	0.10609620207955746	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3458	0.10360933826351588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9962214651103494E-5
2	0.0	0.0	0.0	0.0	2.9962214651103494E-5
3	0.0	0.0	0.0	0.0	2.9962214651103494E-5
4	0.0	0.0	0.0	0.0	2.9962214651103494E-5
5	0.0	0.0	0.0	0.0	5.992442930220699E-5
6	0.0	0.0	0.0	0.0	1.1984885860441398E-4
7	0.0	0.0	0.0	0.0	1.1984885860441398E-4
8	0.0	0.0	0.0	0.0	1.1984885860441398E-4
9	0.0	0.0	0.0	5.992442930220699E-5	1.1984885860441398E-4
10	2.9962214651103494E-5	0.0	0.0	5.992442930220699E-5	1.1984885860441398E-4
11	2.9962214651103494E-5	0.0	0.0	5.992442930220699E-5	1.1984885860441398E-4
12	2.9962214651103494E-5	0.0	0.0	5.992442930220699E-5	4.194710051154489E-4
13	2.9962214651103494E-5	0.0	0.0	5.992442930220699E-5	5.393198637198629E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1020	0.0	15.266672	1
GTATCAA	3300	0.0	13.408269	1
AACCGCG	230	0.0	13.219334	7
CGGTCCA	760	0.0	12.627423	10
GCGTTAT	410	0.0	12.274186	1
CGTTATT	420	0.0	11.984821	2
CAAGACG	905	0.0	11.860951	4
CGCCGGT	770	0.0	11.722501	7
TCGCGTA	390	0.0	11.694552	9
AAGACGG	910	0.0	11.692797	5
CGCGTAA	380	0.0	11.502208	10
CGCATCG	605	0.0	11.463634	13
TATGCCG	75	2.0743665E-4	11.400477	5
TAGATCG	125	1.8333594E-8	11.400477	5
CTATTAG	125	1.848457E-8	11.394151	1
GCCGGTC	835	0.0	11.378746	8
CGGTTCT	795	0.0	11.353699	12
ATCGCCA	615	0.0	11.276218	16
CCGGTCC	895	0.0	11.253556	9
CGCAAGA	870	0.0	11.244055	2
>>END_MODULE