FastQCFastQC Report
Thu 2 Feb 2017
SRR4062133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337537
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT49610.14864254688412443No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT42230.12653043247161005No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT41280.12368402207975522No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC40970.12275519342557101No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA39830.1193395009553452No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA39730.11903987880883418No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC38320.1148152065430286No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG37240.1115792873607094No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA36190.10843325482234355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.027.1189081
GTATCAA34500.026.66121
GTATACG450.001396857424.4500641
TCAACGC51900.017.5060464
ATCAACG52450.017.3644183
CGCAATA10650.017.3524636
ACCGTCG11400.017.1747558
CAACGCA53200.017.1196175
CGTCGTA11850.017.07974410
AACGCAG53650.016.9760256
ATACCGT12600.016.9358336
TACGAAT11150.016.77213941
ACGGACC19600.016.7238248
AGCGAAA18300.016.70971717
GCGAAAG18650.016.63204618
GCGCAAT11650.016.6181135
GTCCTAC7550.016.6130891
ATACGAA11050.016.52571540
GAGCGAA18650.016.39637416
CCGTCGT12000.016.3160159
Produced by FastQC (version 0.11.5)