##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3337537
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204029798021715	32.0	32.0	32.0	32.0	32.0
2	31.291653695524573	32.0	32.0	32.0	32.0	32.0
3	31.473392504712308	32.0	32.0	32.0	32.0	32.0
4	31.5725039153124	32.0	32.0	32.0	32.0	32.0
5	31.528150549342225	32.0	32.0	32.0	32.0	32.0
6	35.1435609552793	36.0	36.0	36.0	36.0	36.0
7	35.14578594933929	36.0	36.0	36.0	36.0	36.0
8	35.084946773623784	36.0	36.0	36.0	36.0	36.0
9	35.204626046093274	36.0	36.0	36.0	36.0	36.0
10	35.04313090761241	36.0	36.0	36.0	36.0	36.0
11	35.19707167291329	36.0	36.0	36.0	36.0	36.0
12	35.100345254599425	36.0	36.0	36.0	36.0	36.0
13	35.153568934217056	36.0	36.0	36.0	36.0	36.0
14	35.10637544992011	36.0	36.0	36.0	36.0	36.0
15	35.07335499201957	36.0	36.0	36.0	36.0	36.0
16	35.089480056700495	36.0	36.0	36.0	36.0	36.0
17	35.066318066286605	36.0	36.0	36.0	36.0	36.0
18	35.067356257024265	36.0	36.0	36.0	36.0	36.0
19	35.05892998339794	36.0	36.0	36.0	36.0	36.0
20	35.06386895486102	36.0	36.0	36.0	36.0	36.0
21	35.05033382401454	36.0	36.0	36.0	36.0	36.0
22	35.02979742247052	36.0	36.0	36.0	36.0	36.0
23	34.99728452448617	36.0	36.0	36.0	36.0	36.0
24	34.97783035813536	36.0	36.0	36.0	36.0	36.0
25	34.959056334057124	36.0	36.0	36.0	32.0	36.0
26	34.91266823408999	36.0	36.0	36.0	32.0	36.0
27	34.90499131545209	36.0	36.0	36.0	32.0	36.0
28	34.87778053097239	36.0	36.0	36.0	32.0	36.0
29	34.856216425465846	36.0	36.0	36.0	32.0	36.0
30	34.83544571940326	36.0	36.0	36.0	32.0	36.0
31	34.84198077804081	36.0	36.0	36.0	32.0	36.0
32	34.81484969305209	36.0	36.0	36.0	32.0	36.0
33	34.792674957610956	36.0	36.0	36.0	32.0	36.0
34	34.78424598738531	36.0	36.0	36.0	32.0	36.0
35	34.7651906181115	36.0	36.0	36.0	32.0	36.0
36	34.745146196132055	36.0	36.0	36.0	32.0	36.0
37	34.737086660013055	36.0	36.0	36.0	32.0	36.0
38	34.71543895992764	36.0	36.0	36.0	32.0	36.0
39	34.70159911335814	36.0	36.0	36.0	32.0	36.0
40	34.689772128368915	36.0	36.0	36.0	32.0	36.0
41	34.65770956247077	36.0	36.0	36.0	32.0	36.0
42	34.622163889119435	36.0	36.0	36.0	32.0	36.0
43	34.6299513683294	36.0	36.0	36.0	32.0	36.0
44	34.58015626493429	36.0	36.0	36.0	32.0	36.0
45	34.56526474463055	36.0	36.0	36.0	32.0	36.0
46	34.55321753736363	36.0	36.0	36.0	32.0	36.0
47	34.533924268105494	36.0	36.0	36.0	32.0	36.0
48	34.51294742200611	36.0	36.0	36.0	32.0	36.0
49	34.492454166051196	36.0	36.0	36.0	32.0	36.0
50	34.04121752058479	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	26.0
21	73.0
22	193.0
23	634.0
24	1605.0
25	3787.0
26	8410.0
27	15956.0
28	28093.0
29	46298.0
30	70409.0
31	106406.0
32	163365.0
33	282813.0
34	630213.0
35	1979249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.826755506615186	17.631179141414307	12.105457027106654	26.436608324863847
2	16.502617004114576	19.387643809379984	36.56108160714136	27.548657579364082
3	18.6684819817296	22.772895756111783	27.99460319296102	30.564019069197602
4	12.738285747843396	15.460592646613355	34.795209760970444	37.00591184457281
5	15.15276085328792	36.17730679839654	32.574020902240186	16.095911446075352
6	36.00503725356424	34.492393034016445	15.802480454874903	13.70008925754442
7	31.71722141207723	29.62052555522231	19.65038889456506	19.011864138135397
8	28.722378208840833	32.81359877059041	18.459870257618118	20.004152762950643
9	27.050216971377395	14.313009863261442	18.202374984906534	40.43439818045463
10	16.458968532474255	26.160167261117184	30.346968542062168	27.033895664346392
11	38.193973579918364	20.872188083607764	21.397455668656256	19.536382667817616
12	24.986899021643804	23.19039459337829	27.808590586411476	24.014115798566426
13	29.284049884690415	18.866517434862896	25.07037375166178	26.77905892878491
14	23.785983496212925	19.3119357178662	24.216091087529517	32.68598969839136
15	25.43345586880385	27.009198699520038	21.41929812313691	26.138047308539203
16	26.487256113967188	25.6102581496092	22.832958104845453	25.069527631578158
17	24.55343566228629	25.980715719406255	24.475803564125282	24.99004505418217
18	25.498459789586974	24.762556512583703	25.30637168613565	24.432612011693674
19	26.028670860380494	24.78185247495532	24.623621924564087	24.565854740100104
20	26.26296916404097	24.087739083849982	24.142809716880617	25.50648203522843
21	27.063768001303956	23.951981341924103	23.96076027344724	25.023490383324702
22	26.312277586735366	23.986370787799505	24.52979547492657	25.171556150538553
23	24.669276975589177	23.999471466375194	25.156372845116877	26.174878712918755
24	25.104359300412167	24.6276263607458	24.7528395437554	25.515174795086633
25	25.214567295404898	24.167925331629878	24.818076596142475	25.799430776822746
26	24.977266019778188	25.216096407057346	25.001295874325656	24.805341698838806
27	25.64756895911666	24.44808586972187	24.523650937327435	25.380694233834035
28	25.055715589518577	24.429465771541814	25.145813345733885	25.369005293205728
29	24.924232550265693	24.69879536121935	25.038778851930253	25.338193236584704
30	24.924411781185327	24.779782177689768	25.213910689197366	25.081895351927542
31	25.685433684032645	24.485321744695614	24.248798959227834	25.580445612043906
32	25.523624213035596	24.399222413848896	24.15733535320365	25.91981801991186
33	24.869738842483923	24.242957208423245	24.893858504772698	25.993445444320134
34	25.681293634687645	24.377058232916685	24.89274192654346	25.048906205852205
35	26.134363315658604	24.119012456206427	24.99414987057806	24.752474357556913
36	24.763852309941782	24.83687038019134	24.67144864615449	25.727828663712387
37	26.047524365630675	24.494260353894905	24.511129328405076	24.94708595206934
38	25.130777576398405	24.184930384292368	24.96448728508478	25.719804754224445
39	25.974610435974334	24.200445061936836	24.177014441591687	25.647930060497142
40	25.98062700803258	24.489076212879393	24.703605926892706	24.82669085219532
41	24.8750560300297	25.017438223146932	25.097018842869023	25.010486903954344
42	26.30788384353665	24.893547212721298	24.484755325635714	24.313813618106337
43	25.652315652399547	23.993368526207984	24.855981480294048	25.498334341098417
44	25.197196506149833	24.622169246963285	24.355435222614442	25.82519902427244
45	25.341590676062197	24.935085636622187	24.453433944354323	25.269889742961293
46	25.15307993595152	24.579412201810232	24.58261822121654	25.68488964102171
47	25.35843434953278	24.435228633639362	25.046134817637505	25.16020219919035
48	26.259195004509483	25.047027485385374	23.846455784575465	24.847321725529678
49	25.293272156501306	25.06534221402163	24.120573051891142	25.52081257758592
50	25.477996519521362	25.117514094500272	24.205027038429087	25.199462347549286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	32.5
2	30.0
3	100.0
4	170.0
5	210.0
6	250.0
7	220.5
8	191.0
9	248.0
10	305.0
11	506.0
12	707.0
13	1445.5
14	2184.0
15	2894.5
16	3605.0
17	3858.0
18	4111.0
19	4283.5
20	4456.0
21	5835.0
22	7214.0
23	8852.0
24	10490.0
25	14162.0
26	17834.0
27	22782.5
28	27731.0
29	32184.5
30	36638.0
31	41861.0
32	47084.0
33	54257.0
34	61430.0
35	71832.5
36	82235.0
37	98385.0
38	114535.0
39	126050.5
40	137566.0
41	150859.0
42	164152.0
43	170663.0
44	177174.0
45	194163.5
46	211153.0
47	226604.5
48	242056.0
49	252170.0
50	262284.0
51	253600.5
52	244917.0
53	244067.5
54	243218.0
55	247830.5
56	252443.0
57	246182.0
58	239921.0
59	220550.5
60	201180.0
61	177557.5
62	153935.0
63	133520.0
64	113105.0
65	96166.5
66	79228.0
67	69956.0
68	60684.0
69	56059.5
70	51435.0
71	40076.0
72	28717.0
73	25311.5
74	21906.0
75	16051.0
76	10196.0
77	9037.5
78	7879.0
79	6778.0
80	5677.0
81	4375.5
82	3074.0
83	2671.5
84	2269.0
85	1769.5
86	1270.0
87	880.5
88	491.0
89	319.5
90	148.0
91	110.5
92	73.0
93	64.5
94	56.0
95	46.5
96	37.0
97	39.0
98	41.0
99	29.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031460325383658665
2	0.003984974548596765
3	2.3969771720882792E-4
4	0.0
5	0.0
6	1.1984885860441396E-4
7	0.0
8	0.0
9	0.0
10	2.996221465110349E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.494332197665524E-4
17	0.0
18	1.7977328790662095E-4
19	5.992442930220698E-5
20	1.4981107325551748E-4
21	2.996221465110349E-5
22	0.0
23	2.996221465110349E-5
24	8.689042248820014E-4
25	1.7977328790662095E-4
26	6.591687223242769E-4
27	4.7939543441765585E-4
28	0.0015280729472062781
29	6.591687223242769E-4
30	0.0013782618739507607
31	4.494332197665524E-4
32	8.689042248820014E-4
33	3.2958436116213845E-4
34	7.790175809286908E-4
35	1.1984885860441396E-4
36	2.0973550255772448E-4
37	0.0014681485179040713
38	0.0
39	7.190931516264838E-4
40	1.1984885860441396E-4
41	0.0012284508006952433
42	0.004254634480456696
43	0.0035655035434813153
44	0.0038950879046434537
45	0.00188761952301952
46	0.0019475439523217268
47	0.0010786397274397258
48	0.0038052012606901438
49	0.003235919182319177
50	0.0019475439523217268
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3337537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.1386184251077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8288393202344	36.02156943230459
2	14.393487965343946	13.857652499401441
3	4.665678788367071	6.737979926619637
4	1.945001763500442	3.745187909172374
5	1.053058267304341	2.5346385054584366
6	0.6338643844081047	1.8308013440572521
7	0.43464344482990713	1.4646194459148874
8	0.32156162489545354	1.238362588879988
9	0.23496901085149954	1.0179975199594795
>10	1.3098866913545217	11.418540314702867
>50	0.09091292223005747	3.013226667656323
>100	0.06962745756757895	6.952920230951287
>500	0.010955806254034445	3.646967928942456
>1k	0.007512552858656694	6.519535685979067
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	4961	0.14864254688412443	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	4223	0.12653043247161005	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	4128	0.12368402207975522	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	4097	0.12275519342557101	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3983	0.1193395009553452	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3973	0.11903987880883418	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3832	0.1148152065430286	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3724	0.1115792873607094	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3619	0.10843325482234355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9962214651103494E-5
2	0.0	0.0	0.0	0.0	2.9962214651103494E-5
3	0.0	0.0	0.0	0.0	2.9962214651103494E-5
4	0.0	0.0	0.0	0.0	2.9962214651103494E-5
5	0.0	0.0	0.0	0.0	2.9962214651103494E-5
6	0.0	0.0	0.0	0.0	8.988664395331047E-5
7	0.0	0.0	0.0	0.0	8.988664395331047E-5
8	0.0	0.0	0.0	0.0	8.988664395331047E-5
9	0.0	0.0	0.0	0.0	1.1984885860441398E-4
10	0.0	0.0	0.0	0.0	1.1984885860441398E-4
11	0.0	0.0	0.0	0.0	1.1984885860441398E-4
12	0.0	0.0	0.0	0.0	4.793954344176559E-4
13	0.0	0.0	0.0	0.0	5.093576490687593E-4
14	0.0	0.0	0.0	0.0	5.393198637198629E-4
15	0.0	0.0	0.0	0.0	6.891309369753804E-4
16	0.0	0.0	0.0	0.0	7.190931516264838E-4
17	0.0	0.0	0.0	0.0	7.790175809286908E-4
18	0.0	0.0	0.0	0.0	7.790175809286908E-4
19	0.0	0.0	0.0	0.0	7.790175809286908E-4
20	0.0	0.0	0.0	0.0	8.389420102308978E-4
21	0.0	0.0	0.0	0.0	8.389420102308978E-4
22	0.0	0.0	0.0	0.0	8.689042248820014E-4
23	0.0	0.0	0.0	0.0	8.988664395331048E-4
24	0.0	0.0	0.0	0.0	9.288286541842082E-4
25	0.0	0.0	0.0	2.9962214651103494E-5	9.587908688353118E-4
26	0.0	0.0	0.0	8.988664395331047E-5	0.0010486775127886224
27	0.0	0.0	0.0	8.988664395331047E-5	0.0010486775127886224
28	0.0	0.0	0.0	2.0973550255772445E-4	0.0010786397274397258
29	0.0	0.0	0.0	3.595465758132419E-4	0.0010786397274397258
30	0.0	0.0	0.0	8.988664395331048E-4	0.0010786397274397258
31	0.0	0.0	0.0	0.0017977328790662096	0.0011685263713930361
32	0.0	0.0	0.0	0.0029962214651103494	0.0012584130153463467
33	0.0	0.0	0.0	0.004194710051154489	0.0012883752299974501
34	0.0	0.0	0.0	0.006232140647429526	0.0014082240886018641
35	0.0	0.0	0.0	0.008239609029053461	0.0014082240886018641
36	0.0	0.0	0.0	0.010996132776954982	0.0014082240886018641
37	0.0	0.0	0.0	0.014861258466947332	0.0014082240886018641
38	0.0	0.0	0.0	0.020793776967865826	0.0014082240886018641
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1420	0.0	27.118908	1
GTATCAA	3450	0.0	26.6612	1
GTATACG	45	0.0013968574	24.450064	1
TCAACGC	5190	0.0	17.506046	4
ATCAACG	5245	0.0	17.364418	3
CGCAATA	1065	0.0	17.35246	36
ACCGTCG	1140	0.0	17.174755	8
CAACGCA	5320	0.0	17.119617	5
CGTCGTA	1185	0.0	17.079744	10
AACGCAG	5365	0.0	16.976025	6
ATACCGT	1260	0.0	16.935833	6
TACGAAT	1115	0.0	16.772139	41
ACGGACC	1960	0.0	16.723824	8
AGCGAAA	1830	0.0	16.709717	17
GCGAAAG	1865	0.0	16.632046	18
GCGCAAT	1165	0.0	16.61811	35
GTCCTAC	755	0.0	16.613089	1
ATACGAA	1105	0.0	16.525715	40
GAGCGAA	1865	0.0	16.396374	16
CCGTCGT	1200	0.0	16.316015	9
>>END_MODULE