FastQCFastQC Report
Thu 2 Feb 2017
SRR4062132_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062132_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173457
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30190.25727402026661395No Hit
TATCAACGCAGAGTACTTTTTTTTT18070.15398945167995076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC551.962231E-413.8159173
CGTCGTA707.2520015E-613.57267910
GTCTAAC707.290453E-613.5657311
GTCTAAA655.4717697E-513.1483251
GGTATCA5500.012.9491071
AATAGCG604.0889878E-412.6672935
GTATTAT951.04432E-611.9949631
GTCCTAG1201.0062649E-811.8700151
GTCCTAA2000.011.8700151
TCCGCAC658.031876E-411.6903913
GTATCAA15650.011.5893821
ACACCGT752.0712122E-411.4010516
ACGGTAT752.0720334E-411.4005639
CCGTCGT855.3173615E-511.1770239
ATTATAC1201.2766941E-711.0815163
CTTAGAC600.005881370511.0815163
TTACACT1303.2694516E-810.9597424
CCCGTAT700.001490314110.85767819
CATAATG700.00149403210.8544372
GTTATAT1053.4807035E-610.8525851
Produced by FastQC (version 0.11.5)