##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062132_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173457
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247093843234136	32.0	32.0	32.0	32.0	32.0
2	30.809589102966704	32.0	32.0	32.0	32.0	32.0
3	30.827853939258105	32.0	32.0	32.0	32.0	32.0
4	30.818077696924558	32.0	32.0	32.0	32.0	32.0
5	30.7583788754083	32.0	32.0	32.0	32.0	32.0
6	34.41664159828609	36.0	36.0	36.0	32.0	36.0
7	34.352718506089275	36.0	36.0	36.0	32.0	36.0
8	34.3109930743095	36.0	36.0	36.0	32.0	36.0
9	34.42866419476811	36.0	36.0	36.0	32.0	36.0
10	34.123025385676684	36.0	36.0	36.0	32.0	36.0
11	34.42789552578407	36.0	36.0	36.0	32.0	36.0
12	34.261988296119924	36.0	36.0	36.0	32.0	36.0
13	34.33162527472246	36.0	36.0	36.0	32.0	36.0
14	34.237713013770424	36.0	36.0	36.0	32.0	36.0
15	34.19492490990296	36.0	36.0	36.0	32.0	36.0
16	34.19676392062087	36.0	36.0	36.0	32.0	36.0
17	34.143288590889995	36.0	36.0	36.0	32.0	36.0
18	34.1389211534807	36.0	36.0	36.0	32.0	36.0
19	34.139764814560735	36.0	36.0	36.0	32.0	36.0
20	34.122542197967206	36.0	36.0	36.0	32.0	36.0
21	34.10294369542301	36.0	36.0	36.0	32.0	36.0
22	34.077595514790914	36.0	36.0	36.0	32.0	36.0
23	34.046610996397824	36.0	36.0	36.0	32.0	36.0
24	34.02739512397983	36.0	36.0	36.0	32.0	36.0
25	33.6737656343607	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	16.0
5	85.0
6	252.0
7	42.0
8	207.0
9	155.0
10	71.0
11	20.0
12	42.0
13	59.0
14	208.0
15	292.0
16	528.0
17	622.0
18	872.0
19	1115.0
20	1678.0
21	2296.0
22	3460.0
23	4951.0
24	7004.0
25	9682.0
26	13310.0
27	17377.0
28	22669.0
29	30171.0
30	39678.0
31	53754.0
32	76244.0
33	108217.0
34	240043.0
35	538335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33177552571746	17.04486963161429	11.633362989627114	25.989991853041133
2	16.394820086990904	19.40430659521959	38.02281466028551	26.17805865750399
3	19.307204068693288	23.289229688916766	28.123359535318137	29.28020670707181
4	13.00141306737894	15.498655966672747	35.319440159409	36.18049080653932
5	14.887764009465808	36.25121902982276	33.48555577530297	15.375461185408469
6	34.343798684604344	34.71541117412663	16.893701603186422	14.047088538082605
7	30.305227598444006	30.266498327987446	20.382430219067768	19.045843854500784
8	27.807720655979363	33.471957769451784	19.264957789925543	19.45536378464331
9	27.204016687853528	14.821902381175828	18.540580662224535	39.43350026874611
10	16.08037885575323	27.247152182260336	31.69247834805239	24.979990613934042
11	36.22617776742384	21.678071641847087	22.27123275280175	19.824517837927324
12	24.564041172159044	23.532433357196552	29.066595446761273	22.836930023883127
13	29.58609821048419	20.27426784972265	24.875063246964785	25.26457069282837
14	23.230749674316147	19.542737314112795	25.611163768428757	31.615349243142298
15	24.88316510986362	27.34537989151442	22.466515295408605	25.304939703213353
16	25.053343559826263	26.191879834726677	24.031689615132933	24.723086990314123
17	23.757670177258735	26.098055409565283	25.636309032209624	24.507965380966358
18	23.972277862897585	25.867681734578625	26.136150362906115	24.023890039617676
19	25.359727111512203	25.2380054710764	25.554549975391243	23.84771744202015
20	25.54547478333258	25.148688310237212	25.332922786137036	23.972914120293172
21	25.522592509194624	24.747361022974623	25.24922724202166	24.480819225809093
22	25.345222334443413	24.911199295736203	25.244137959272805	24.499440410547578
23	24.380685471972	25.087121089359034	25.632278214354553	24.899915224314416
24	24.436163334794706	25.194001436352686	25.70414546397433	24.665689764878277
25	24.64576767749997	24.958868708234363	25.576200568886918	24.819163045378755
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	444.0
1	444.0
2	483.5
3	523.0
4	523.0
5	523.0
6	1153.5
7	1784.0
8	1784.0
9	1784.0
10	2113.0
11	2442.0
12	2442.0
13	2442.0
14	3166.5
15	3891.0
16	3891.0
17	3891.0
18	6374.5
19	8858.0
20	8858.0
21	8858.0
22	13065.5
23	17273.0
24	17273.0
25	17273.0
26	25213.0
27	33153.0
28	33153.0
29	33153.0
30	42769.0
31	52385.0
32	52385.0
33	52385.0
34	62651.0
35	72917.0
36	72917.0
37	72917.0
38	84889.0
39	96861.0
40	96861.0
41	96861.0
42	110336.0
43	123811.0
44	123811.0
45	123811.0
46	136931.0
47	150051.0
48	150051.0
49	150051.0
50	155075.5
51	160100.0
52	160100.0
53	160100.0
54	152674.0
55	145248.0
56	145248.0
57	145248.0
58	131431.5
59	117615.0
60	117615.0
61	117615.0
62	101314.0
63	85013.0
64	85013.0
65	85013.0
66	68690.5
67	52368.0
68	52368.0
69	52368.0
70	39257.5
71	26147.0
72	26147.0
73	26147.0
74	19371.0
75	12595.0
76	12595.0
77	12595.0
78	9161.5
79	5728.0
80	5728.0
81	5728.0
82	4038.5
83	2349.0
84	2349.0
85	2349.0
86	1677.0
87	1005.0
88	1005.0
89	1005.0
90	659.5
91	314.0
92	314.0
93	314.0
94	216.0
95	118.0
96	118.0
97	118.0
98	291.0
99	464.0
100	464.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011078377818701495
2	8.521829091308841E-5
3	7.669646182177958E-4
4	0.010481849782309876
5	0.03349078832884375
6	0.062209352366554546
7	0.10371066004122861
8	0.10439240636853332
9	0.1147890378599301
10	0.12842396440602427
11	0.13634926546094148
12	0.12782743636963265
13	0.12475957789676145
14	0.11146552451431965
15	0.128168309533285
16	0.1140220732417123
17	0.11802733291462746
18	0.1067785185140998
19	0.09459230291352816
20	0.08845658596778577
21	0.08803049451322033
22	0.09962018207740037
23	0.0810425946583471
24	0.08888267742235123
25	0.08453654458578372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1173457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.765398823276705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05491860097382	47.593277786842705
2	13.921246782471128	17.19702719273174
3	4.247117128081183	7.869746497953117
4	1.6599142827572761	4.101010707478259
5	0.8779355244545626	2.7113018904529333
6	0.5090696957107843	1.8865735670652424
7	0.35987006759387974	1.555926277464687
8	0.2494928068871371	1.2328018176738225
9	0.16559108164524408	0.9205019279456649
>10	0.8800959993367342	9.601150847557841
>50	0.050320362330089866	2.1428646421010047
>100	0.023456115971621935	2.4168818530251137
>500	5.551724494745766E-4	0.26969437161267984
>1k	4.163793371059325E-4	0.501240620095149
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3019	0.25727402026661395	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1807	0.15398945167995076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5565487273926526E-4
2	0.0	0.0	0.0	0.0	2.5565487273926526E-4
3	0.0	0.0	0.0	0.0	2.5565487273926526E-4
4	0.0	0.0	0.0	0.0	2.5565487273926526E-4
5	0.0	8.521829091308843E-5	0.0	0.0	2.5565487273926526E-4
6	0.0	8.521829091308843E-5	0.0	0.0	2.5565487273926526E-4
7	0.0	8.521829091308843E-5	0.0	0.0	2.5565487273926526E-4
8	0.0	8.521829091308843E-5	0.0	0.0	2.5565487273926526E-4
9	0.0	8.521829091308843E-5	0.0	0.0	2.5565487273926526E-4
10	0.0	8.521829091308843E-5	0.0	0.0	3.408731636523537E-4
11	0.0	8.521829091308843E-5	0.0	1.7043658182617686E-4	3.408731636523537E-4
12	0.0	8.521829091308843E-5	0.0	1.7043658182617686E-4	5.965280363916189E-4
13	0.0	8.521829091308843E-5	0.0	1.7043658182617686E-4	7.669646182177958E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAGAC	55	1.962231E-4	13.815917	3
CGTCGTA	70	7.2520015E-6	13.572679	10
GTCTAAC	70	7.290453E-6	13.565731	1
GTCTAAA	65	5.4717697E-5	13.148325	1
GGTATCA	550	0.0	12.949107	1
AATAGCG	60	4.0889878E-4	12.667293	5
GTATTAT	95	1.04432E-6	11.994963	1
GTCCTAG	120	1.0062649E-8	11.870015	1
GTCCTAA	200	0.0	11.870015	1
TCCGCAC	65	8.031876E-4	11.690391	3
GTATCAA	1565	0.0	11.589382	1
ACACCGT	75	2.0712122E-4	11.401051	6
ACGGTAT	75	2.0720334E-4	11.400563	9
CCGTCGT	85	5.3173615E-5	11.177023	9
ATTATAC	120	1.2766941E-7	11.081516	3
CTTAGAC	60	0.0058813705	11.081516	3
TTACACT	130	3.2694516E-8	10.959742	4
CCCGTAT	70	0.0014903141	10.857678	19
CATAATG	70	0.001494032	10.854437	2
GTTATAT	105	3.4807035E-6	10.852585	1
>>END_MODULE