FastQCFastQC Report
Thu 2 Feb 2017
SRR4062132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173457
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.1015802027684014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG1650.027.99959413
GCGCTAT1700.025.88197711
GTATCAA17800.025.4668161
CGCTATC1750.025.14249212
CTATCGG2050.022.53625714
TAGATTG904.0821578E-721.999685
GTAGGAC4150.021.7346253
TGCGCTA2150.021.48806210
TTGTGCG2100.020.9520767
GTTCCGC654.9311115E-420.30826241
TATACTG1750.020.1139955
TATCGGT2200.019.9997115
ATATACT2350.018.7231314
TATCAAC24950.018.4285892
TCCGCGT600.007407132518.33541144
GTACGCT600.00741259618.3330693
AACGCAG24400.018.3030136
TAGGACG2650.018.2638854
TCAACGC24950.018.075894
TACCGTC1102.7966726E-617.9997397
Produced by FastQC (version 0.11.5)