##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1173457
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284072616210054	32.0	32.0	32.0	32.0	32.0
2	31.492178239168542	32.0	32.0	32.0	32.0	32.0
3	31.5964982099898	32.0	32.0	32.0	32.0	32.0
4	31.674404771542545	32.0	32.0	32.0	32.0	32.0
5	31.65488637419181	32.0	32.0	32.0	32.0	32.0
6	35.3077641532668	36.0	36.0	36.0	36.0	36.0
7	35.311731916891716	36.0	36.0	36.0	36.0	36.0
8	35.269225885567174	36.0	36.0	36.0	36.0	36.0
9	35.34559851788349	36.0	36.0	36.0	36.0	36.0
10	35.23628475521472	36.0	36.0	36.0	36.0	36.0
11	35.349386470914574	36.0	36.0	36.0	36.0	36.0
12	35.28123484712265	36.0	36.0	36.0	36.0	36.0
13	35.299000304229295	36.0	36.0	36.0	36.0	36.0
14	35.271531892519285	36.0	36.0	36.0	36.0	36.0
15	35.24838404815856	36.0	36.0	36.0	36.0	36.0
16	35.263230778801436	36.0	36.0	36.0	36.0	36.0
17	35.25183794548927	36.0	36.0	36.0	36.0	36.0
18	35.237844249938426	36.0	36.0	36.0	36.0	36.0
19	35.229497118343495	36.0	36.0	36.0	36.0	36.0
20	35.22750812343358	36.0	36.0	36.0	36.0	36.0
21	35.22300348457591	36.0	36.0	36.0	36.0	36.0
22	35.20596834822239	36.0	36.0	36.0	36.0	36.0
23	35.180674707296475	36.0	36.0	36.0	36.0	36.0
24	35.16426762974698	36.0	36.0	36.0	36.0	36.0
25	35.15154283454783	36.0	36.0	36.0	36.0	36.0
26	35.111442515575774	36.0	36.0	36.0	36.0	36.0
27	35.095716332170674	36.0	36.0	36.0	36.0	36.0
28	35.07946605627645	36.0	36.0	36.0	36.0	36.0
29	35.058835560229305	36.0	36.0	36.0	36.0	36.0
30	35.03435404961579	36.0	36.0	36.0	36.0	36.0
31	35.030675175997075	36.0	36.0	36.0	36.0	36.0
32	35.014941322945795	36.0	36.0	36.0	36.0	36.0
33	34.99273173196802	36.0	36.0	36.0	36.0	36.0
34	34.974744707305	36.0	36.0	36.0	36.0	36.0
35	34.97081699627681	36.0	36.0	36.0	36.0	36.0
36	34.93905017397314	36.0	36.0	36.0	36.0	36.0
37	34.92188209708579	36.0	36.0	36.0	36.0	36.0
38	34.90289290532163	36.0	36.0	36.0	36.0	36.0
39	34.87535887552761	36.0	36.0	36.0	36.0	36.0
40	34.87009664606372	36.0	36.0	36.0	36.0	36.0
41	34.84519500927601	36.0	36.0	36.0	32.0	36.0
42	34.82454235647322	36.0	36.0	36.0	32.0	36.0
43	34.80665162847893	36.0	36.0	36.0	32.0	36.0
44	34.76677969452651	36.0	36.0	36.0	32.0	36.0
45	34.73669934220001	36.0	36.0	36.0	32.0	36.0
46	34.70927268745255	36.0	36.0	36.0	32.0	36.0
47	34.67220273090535	36.0	36.0	36.0	32.0	36.0
48	34.657032170757006	36.0	36.0	36.0	32.0	36.0
49	34.626632249839574	36.0	36.0	36.0	32.0	36.0
50	34.23610238807217	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	22.0
21	38.0
22	76.0
23	200.0
24	538.0
25	1378.0
26	2697.0
27	5204.0
28	8872.0
29	14097.0
30	21121.0
31	31062.0
32	46221.0
33	77515.0
34	176733.0
35	787676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76916486579799	17.68261234649992	12.01556632524754	26.53265646245455
2	15.824127874770538	19.762689215423922	37.747805944785995	26.665376965019544
3	18.758926654008857	23.67808823880312	28.334179838629957	29.228805268558066
4	12.732390477359184	15.827095349122592	35.368944383087225	36.071569790431
5	14.559459784210244	36.46524755487419	33.48567523138897	15.489617429526604
6	34.53238513620037	34.83550711360896	16.768176027201726	13.863931722988951
7	30.313850443603812	30.252493274146392	20.38327778521071	19.05037849703909
8	27.795309073958396	33.545242816737215	19.256606761048765	19.402841348255624
9	27.389670009212097	14.437597628204527	18.471149773702827	39.70158258888055
10	15.960107613657765	27.34211820288259	31.898825436296345	24.798948747163298
11	36.51083934051269	21.47799195028024	22.24785399038908	19.763314718817988
12	24.61777466068207	23.397448734806645	29.080912210673247	22.903864393838035
13	29.682808999392396	20.20730201447518	24.860732008075285	25.24915697805714
14	23.173835939450697	19.499393671860155	25.762767617390324	31.56400277129882
15	24.988133352990353	27.18284521716603	22.54995283167598	25.279068598167637
16	25.133665686650474	26.101751246324937	24.09280327240189	24.671779794622694
17	23.811183537189688	25.91437095692471	25.733452525316224	24.540992980569378
18	24.072674282354246	25.67887136703154	26.15831028884789	24.09014406176632
19	25.45163563726664	25.130447898815213	25.574349976181487	23.843566487736663
20	25.615320242634137	25.08117062961139	25.457154690341504	23.84635443741297
21	25.587644029563926	24.563320172788607	25.41303175148301	24.436004046164452
22	25.327387369115357	24.745176005597138	25.395732438427654	24.53170418685985
23	24.503559577111673	24.80736356090652	25.682642864569043	25.006433997412763
24	24.701179261764068	24.933742157713265	25.812010096757753	24.55306848376491
25	24.797371863428936	24.727237090472155	25.715089202398044	24.760301843700866
26	24.519366791313153	25.09791623347184	25.909368257074	24.473348718141004
27	24.7041198246535	25.191231311485467	25.588777687635073	24.51587117622596
28	24.515994880002182	25.016660445630702	25.587887898636314	24.8794567757308
29	24.542502876134474	25.199284162086155	25.511270186203078	24.74694277557629
30	24.651684416579386	25.073779244319873	25.62072142167469	24.653814917426047
31	24.88493321823104	24.89618210932047	25.442264640389144	24.77662003205934
32	24.635986658099306	25.139120164029706	25.51860077072145	24.70629240714954
33	24.493738575403405	25.226531907912957	25.609588778436326	24.670140738247312
34	24.71907527061322	25.138864506760427	25.83808713822366	24.303973084402692
35	24.621757648787256	25.047509318643936	25.871913172565776	24.458819860003032
36	24.570710298691893	25.15889045123354	25.60986833695513	24.660530913119434
37	24.84737078927454	25.179217883950667	25.294861568459016	24.67854975831578
38	24.776536336653155	25.23841947340209	25.485467298759133	24.49957689118562
39	25.012931963809248	24.996143846046994	25.291256799400742	24.699667390743016
40	24.98321198238366	24.944352408611827	25.72819091640419	24.34424469260032
41	24.664596966693626	25.067089128621088	25.68169287027276	24.586621034412527
42	24.837799994375306	25.25172639780057	25.46052188645446	24.449951721369665
43	24.697845417528118	25.064726105030992	25.510181471715672	24.727247005725214
44	24.489863747771455	25.247057299567416	25.469144682345163	24.79393427031597
45	24.54664840676071	25.36067433253198	25.401750598886853	24.690926661820463
46	24.642434995734703	25.179666667518873	25.317042532829166	24.860855803917257
47	24.771994770921	25.293283496869883	25.45111032709014	24.483611405118975
48	24.796809978447413	25.545824175543224	25.051367426021105	24.605998419988257
49	24.721027702072757	25.393935985082845	25.026205584729688	24.858830728114715
50	24.563568389270152	25.77036517512231	25.18192284715754	24.484143588449996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	201.0
1	137.0
2	73.0
3	222.5
4	372.0
5	354.0
6	336.0
7	281.0
8	226.0
9	305.0
10	384.0
11	593.5
12	803.0
13	1489.5
14	2176.0
15	2799.5
16	3423.0
17	3261.5
18	3100.0
19	3094.5
20	3089.0
21	3422.0
22	3755.0
23	4478.5
24	5202.0
25	6534.5
26	7867.0
27	9523.0
28	11179.0
29	13414.5
30	15650.0
31	18580.0
32	21510.0
33	24856.0
34	28202.0
35	32192.5
36	36183.0
37	40060.5
38	43938.0
39	47503.5
40	51069.0
41	55294.0
42	59519.0
43	63045.5
44	66572.0
45	71036.5
46	75501.0
47	79003.5
48	82506.0
49	85274.5
50	88043.0
51	89232.0
52	90421.0
53	90285.5
54	90150.0
55	87144.5
56	84139.0
57	80359.5
58	76580.0
59	70888.0
60	65196.0
61	57404.5
62	49613.0
63	42536.5
64	35460.0
65	29814.0
66	24168.0
67	20501.0
68	16834.0
69	14195.5
70	11557.0
71	9260.5
72	6964.0
73	5846.0
74	4728.0
75	3680.5
76	2633.0
77	2208.5
78	1784.0
79	1457.0
80	1130.0
81	876.0
82	622.0
83	462.0
84	302.0
85	229.5
86	157.0
87	112.0
88	67.0
89	51.5
90	36.0
91	23.0
92	10.0
93	10.0
94	10.0
95	7.5
96	5.0
97	7.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03510993585619243
2	0.005027879163872217
3	4.260914545654421E-4
4	8.521829091308841E-5
5	0.0
6	1.7043658182617683E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.965280363916189E-4
17	0.0
18	1.7043658182617683E-4
19	0.0
20	2.5565487273926526E-4
21	0.0
22	0.0
23	2.5565487273926526E-4
24	9.374012000439727E-4
25	1.7043658182617683E-4
26	4.260914545654421E-4
27	7.669646182177958E-4
28	0.00161914752734868
29	5.965280363916189E-4
30	0.002045238981914122
31	6.817463273047073E-4
32	9.374012000439727E-4
33	1.7043658182617683E-4
34	9.374012000439727E-4
35	2.5565487273926526E-4
36	5.965280363916189E-4
37	0.0021304572728272106
38	0.0
39	6.817463273047073E-4
40	8.521829091308841E-5
41	0.0015339292364355917
42	0.005113097454785305
43	0.00434613283656751
44	0.0038348230910889786
45	0.0025565487273926527
46	0.0028974218910450063
47	0.00161914752734868
48	0.004090477963828244
49	0.003664386509262802
50	0.0017043658182617685
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1173457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.49910676049942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.73359519324747	48.72514753292966
2	14.347468774213937	18.221029028734854
3	4.2875735013172	8.167712625108877
4	1.6774193538345177	4.260585225250639
5	0.8438578143508716	2.679210872207385
6	0.5051189224228213	1.9244760229005093
7	0.34323584948638325	1.5256618895396594
8	0.23278598982319118	1.182536193610508
9	0.1711757215859383	0.978255487781192
>10	0.801126200540918	8.813054161362802
>50	0.04057556586691883	1.7768542376023637
>100	0.015527045291906417	1.488444416688604
>500	4.050510134522522E-4	0.15483667283085137
>1k	1.3501700448408408E-4	0.10219563345203479
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.1015802027684014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.521829091308843E-5	0.0
5	0.0	8.521829091308843E-5	0.0	8.521829091308843E-5	0.0
6	0.0	8.521829091308843E-5	0.0	8.521829091308843E-5	0.0
7	0.0	8.521829091308843E-5	0.0	8.521829091308843E-5	0.0
8	0.0	8.521829091308843E-5	0.0	8.521829091308843E-5	0.0
9	0.0	8.521829091308843E-5	0.0	8.521829091308843E-5	0.0
10	0.0	8.521829091308843E-5	0.0	8.521829091308843E-5	0.0
11	0.0	8.521829091308843E-5	0.0	8.521829091308843E-5	0.0
12	0.0	1.7043658182617686E-4	0.0	8.521829091308843E-5	8.521829091308843E-5
13	0.0	1.7043658182617686E-4	0.0	8.521829091308843E-5	3.408731636523537E-4
14	0.0	1.7043658182617686E-4	0.0	1.7043658182617686E-4	3.408731636523537E-4
15	0.0	1.7043658182617686E-4	0.0	3.408731636523537E-4	4.260914545654421E-4
16	0.0	1.7043658182617686E-4	0.0	5.113097454785305E-4	5.113097454785305E-4
17	0.0	1.7043658182617686E-4	0.0	7.669646182177958E-4	5.113097454785305E-4
18	0.0	1.7043658182617686E-4	0.0	0.001022619490957061	5.965280363916189E-4
19	0.0	1.7043658182617686E-4	0.0	0.001022619490957061	5.965280363916189E-4
20	0.0	1.7043658182617686E-4	0.0	0.0011930560727832378	6.817463273047074E-4
21	0.0	1.7043658182617686E-4	0.0	0.0014487109455225031	7.669646182177958E-4
22	0.0	1.7043658182617686E-4	0.0	0.0019600206910010336	7.669646182177958E-4
23	0.0	2.5565487273926526E-4	0.0	0.0023861121455664757	7.669646182177958E-4
24	0.0	2.5565487273926526E-4	0.0	0.00323829505469736	7.669646182177958E-4
25	0.0	2.5565487273926526E-4	0.0	0.003834823091088979	7.669646182177958E-4
26	0.0	2.5565487273926526E-4	0.0	0.00485744258204604	8.521829091308842E-4
27	0.0	2.5565487273926526E-4	0.0	0.005453970618437659	9.374012000439726E-4
28	0.0	2.5565487273926526E-4	0.0	0.006561808400307808	9.374012000439726E-4
29	0.0	2.5565487273926526E-4	0.0	0.00920357541861355	9.374012000439726E-4
30	0.0	2.5565487273926526E-4	0.0	0.012527088764223999	9.374012000439726E-4
31	0.0	2.5565487273926526E-4	0.0	0.019344552037271073	9.374012000439726E-4
32	0.0	2.5565487273926526E-4	0.0	0.028207254292232267	9.374012000439726E-4
33	0.0	2.5565487273926526E-4	0.0	0.03630299192897567	0.001022619490957061
34	0.0	2.5565487273926526E-4	0.0	0.046699623420372455	0.001022619490957061
35	0.0	2.5565487273926526E-4	0.0	0.057351909784508506	0.001022619490957061
36	0.0	2.5565487273926526E-4	0.0	0.07090161803968957	0.001022619490957061
37	0.0	2.5565487273926526E-4	0.0	0.09101313469517844	0.001022619490957061
38	0.0	2.5565487273926526E-4	0.0	0.11947604386014997	0.001022619490957061
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTATCG	165	0.0	27.999594	13
GCGCTAT	170	0.0	25.881977	11
GTATCAA	1780	0.0	25.466816	1
CGCTATC	175	0.0	25.142492	12
CTATCGG	205	0.0	22.536257	14
TAGATTG	90	4.0821578E-7	21.99968	5
GTAGGAC	415	0.0	21.734625	3
TGCGCTA	215	0.0	21.488062	10
TTGTGCG	210	0.0	20.952076	7
GTTCCGC	65	4.9311115E-4	20.308262	41
TATACTG	175	0.0	20.113995	5
TATCGGT	220	0.0	19.99971	15
ATATACT	235	0.0	18.723131	4
TATCAAC	2495	0.0	18.428589	2
TCCGCGT	60	0.0074071325	18.335411	44
GTACGCT	60	0.007412596	18.333069	3
AACGCAG	2440	0.0	18.303013	6
TAGGACG	265	0.0	18.263885	4
TCAACGC	2495	0.0	18.07589	4
TACCGTC	110	2.7966726E-6	17.999739	7
>>END_MODULE