FastQCFastQC Report
Thu 2 Feb 2017
SRR4062131_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062131_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433197
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21310.14868856130734295No Hit
GCGCAAGACGGACCAGAGCGAAAGC16700.11652271111368499No Hit
GAATAGGACCGCGGTTCTATTTTGT14470.10096309160568995No Hit
TATCAACGCAGAGTACTTTTTTTTT14410.1005444471346228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3350.015.0294085
CAAGACG3400.014.5289844
GTATAGG759.731721E-713.9256231
GGTATCA6650.013.4211411
CGCAAGA3200.013.3543152
GCAAGAC3850.013.3219553
CGGTTCT2500.013.30286412
CGTTATT1153.8016879E-1013.2123852
GCGCAAG3550.012.3030481
TCGCGTA853.933219E-612.2967639
ACACCGT853.936295E-612.2959046
GTAATAC853.9517054E-612.2916073
CGGACAT1251.4097168E-912.1596425
TCCAACG951.0421372E-611.9975518
CGGACCA3650.011.9751819
GCGTTAT1353.7471182E-1011.9563431
GGTTCTA2650.011.83149313
GTCCTAT2100.011.7553961
CGGTCCA2600.011.69523310
GTGCGCA658.008265E-411.6948259
Produced by FastQC (version 0.11.5)