##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062131_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1433197
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264436780149556	32.0	32.0	32.0	32.0	32.0
2	30.784984897400705	32.0	32.0	32.0	32.0	32.0
3	30.816454402290823	32.0	32.0	32.0	32.0	32.0
4	30.827755012046495	32.0	32.0	32.0	32.0	32.0
5	30.741554719972203	32.0	32.0	32.0	32.0	32.0
6	34.39793761778736	36.0	36.0	36.0	32.0	36.0
7	34.331435245817566	36.0	36.0	36.0	32.0	36.0
8	34.293704912862644	36.0	36.0	36.0	32.0	36.0
9	34.43135661043109	36.0	36.0	36.0	32.0	36.0
10	34.11616058364621	36.0	36.0	36.0	32.0	36.0
11	34.40108163776508	36.0	36.0	36.0	32.0	36.0
12	34.22792819130936	36.0	36.0	36.0	32.0	36.0
13	34.32054351216197	36.0	36.0	36.0	32.0	36.0
14	34.22077774374353	36.0	36.0	36.0	32.0	36.0
15	34.16198610519	36.0	36.0	36.0	32.0	36.0
16	34.17172168236467	36.0	36.0	36.0	32.0	36.0
17	34.111614802431205	36.0	36.0	36.0	32.0	36.0
18	34.11912528424215	36.0	36.0	36.0	32.0	36.0
19	34.11791819268391	36.0	36.0	36.0	32.0	36.0
20	34.101520586492995	36.0	36.0	36.0	32.0	36.0
21	34.08243039861233	36.0	36.0	36.0	32.0	36.0
22	34.06154492369158	36.0	36.0	36.0	32.0	36.0
23	34.02542497646869	36.0	36.0	36.0	32.0	36.0
24	33.99428271200679	36.0	36.0	36.0	32.0	36.0
25	33.64282718984201	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	22.0
5	107.0
6	330.0
7	72.0
8	252.0
9	195.0
10	106.0
11	35.0
12	65.0
13	77.0
14	177.0
15	282.0
16	439.0
17	617.0
18	871.0
19	1233.0
20	1857.0
21	2667.0
22	4108.0
23	5948.0
24	8383.0
25	12217.0
26	16594.0
27	21465.0
28	28514.0
29	37767.0
30	49925.0
31	67939.0
32	95387.0
33	135011.0
34	295879.0
35	644656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33272489657622	17.297815139888122	11.485569512852878	25.883890450682784
2	16.974581179048137	19.233737327221096	37.28703102868426	26.504650465046502
3	19.32017198046869	22.74652417760151	28.034044429683235	29.899259412246558
4	12.938890920306703	15.403295362877904	35.04232947488005	36.61548424193535
5	15.17670182648657	36.20042214119395	33.1524622111041	15.470413821215386
6	35.22613023224639	34.718894176525126	16.335933398125498	13.719042193102988
7	31.038623299713535	29.939012166133345	20.01394247124016	19.00842206291296
8	28.3572497818845	33.11660325192916	18.855866346368106	19.67028061981823
9	26.958197137601758	14.427048804120556	18.574710941369528	40.04004311690816
10	16.59395419813193	26.547573797798783	30.737041715809305	26.12143028825999
11	37.31281125337064	21.294953773611933	21.758230854533092	19.634004118484334
12	24.54505312080354	23.565291147836952	28.407857099938095	23.481798631421412
13	29.441987522094838	19.348927221535213	24.989345573697193	26.21973968267276
14	23.5741989387178	19.578323352958765	24.698842313807567	32.148635394515864
15	25.258078782937144	27.13818490004185	21.761133108215272	25.842603208805738
16	25.862291792850172	25.845246104117024	23.459338697136534	24.83312340589627
17	24.20575066212188	26.033423618975494	24.873356746587042	24.887468972315585
18	24.90044112562126	25.143811143748273	25.683290664412723	24.27245706621774
19	25.64640360728833	25.069005593084913	24.96451939140978	24.32007140821697
20	25.797701268234498	24.484140259373824	24.592393107296733	25.125765365094953
21	26.546480856386758	24.296139848007694	24.314159338391363	24.843219957214185
22	26.005988889750363	24.349300213529695	24.64944319530388	24.995267701416058
23	24.627437890020502	24.40508779476751	25.282895802722948	25.684578512489036
24	24.794531263094967	24.93581720964795	25.095680547097178	25.173970980159904
25	24.995652535252876	24.534716513871032	25.008921905565384	25.460709045310708
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	509.0
1	509.0
2	463.5
3	418.0
4	418.0
5	418.0
6	933.5
7	1449.0
8	1449.0
9	1449.0
10	1796.5
11	2144.0
12	2144.0
13	2144.0
14	3024.0
15	3904.0
16	3904.0
17	3904.0
18	6573.0
19	9242.0
20	9242.0
21	9242.0
22	14121.0
23	19000.0
24	19000.0
25	19000.0
26	28175.0
27	37350.0
28	37350.0
29	37350.0
30	47047.0
31	56744.0
32	56744.0
33	56744.0
34	70968.5
35	85193.0
36	85193.0
37	85193.0
38	99476.5
39	113760.0
40	113760.0
41	113760.0
42	130782.0
43	147804.0
44	147804.0
45	147804.0
46	165259.5
47	182715.0
48	182715.0
49	182715.0
50	189291.0
51	195867.0
52	195867.0
53	195867.0
54	184344.5
55	172822.0
56	172822.0
57	172822.0
58	159382.0
59	145942.0
60	145942.0
61	145942.0
62	129060.0
63	112178.0
64	112178.0
65	112178.0
66	91977.0
67	71776.0
68	71776.0
69	71776.0
70	54112.0
71	36448.0
72	36448.0
73	36448.0
74	27795.0
75	19142.0
76	19142.0
77	19142.0
78	15095.0
79	11048.0
80	11048.0
81	11048.0
82	7684.0
83	4320.0
84	4320.0
85	4320.0
86	3168.0
87	2016.0
88	2016.0
89	2016.0
90	1324.0
91	632.0
92	632.0
93	632.0
94	399.0
95	166.0
96	166.0
97	166.0
98	387.0
99	608.0
100	608.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001116385256179018
2	4.884185495783204E-4
3	7.675148636230748E-4
4	0.01067543401221186
5	0.0349568133341055
6	0.06454102262284947
7	0.1116385256179018
8	0.11275491087408081
9	0.12140689660946821
10	0.13878064215875416
11	0.14568827593136183
12	0.13473374560510523
13	0.13026820458038915
14	0.12008118911775562
15	0.13591990493979544
16	0.12210463739458009
17	0.12657017841929616
18	0.11387129613025984
19	0.09970715819248854
20	0.09517184308926127
21	0.0986605470148207
22	0.10787072537829762
23	0.08519414986216131
24	0.09412523191159344
25	0.09265997626285849
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1433197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.67401464677798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80677197126175	39.930470171391995
2	14.168865741622383	14.926620832048961
3	4.425854833392248	6.993826269558491
4	1.8392213305808491	3.8751668522272844
5	0.9717248231537483	2.559232378371914
6	0.6000784688764965	1.896512523529001
7	0.40188641995156094	1.481827981960878
8	0.2892455142509466	1.2188577963335352
9	0.20411920524555077	0.9676600206113538
>10	1.1221328076782378	10.634482009176407
>50	0.08727467893928434	3.203808438938929
>100	0.07286942783702863	7.917623753942495
>500	0.007565630679611436	2.72400527133367
>1k	0.0023891465304036116	1.66990570057504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2131	0.14868856130734295	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1670	0.11652271111368499	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1447	0.10096309160568995	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1441	0.1005444471346228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3954815702237725E-4
2	0.0	0.0	0.0	0.0	1.3954815702237725E-4
3	0.0	0.0	0.0	0.0	1.3954815702237725E-4
4	0.0	0.0	0.0	0.0	1.3954815702237725E-4
5	0.0	0.0	0.0	0.0	2.0932223553356586E-4
6	0.0	0.0	0.0	0.0	2.0932223553356586E-4
7	0.0	0.0	0.0	0.0	2.0932223553356586E-4
8	0.0	0.0	0.0	0.0	2.0932223553356586E-4
9	0.0	6.977407851118863E-5	0.0	0.0	2.0932223553356586E-4
10	0.0	6.977407851118863E-5	0.0	0.0	3.488703925559431E-4
11	0.0	6.977407851118863E-5	0.0	0.0	3.488703925559431E-4
12	0.0	6.977407851118863E-5	0.0	0.0	4.884185495783204E-4
13	0.0	6.977407851118863E-5	0.0	0.0	5.58192628089509E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	335	0.0	15.029408	5
CAAGACG	340	0.0	14.528984	4
GTATAGG	75	9.731721E-7	13.925623	1
GGTATCA	665	0.0	13.421141	1
CGCAAGA	320	0.0	13.354315	2
GCAAGAC	385	0.0	13.321955	3
CGGTTCT	250	0.0	13.302864	12
CGTTATT	115	3.8016879E-10	13.212385	2
GCGCAAG	355	0.0	12.303048	1
TCGCGTA	85	3.933219E-6	12.296763	9
ACACCGT	85	3.936295E-6	12.295904	6
GTAATAC	85	3.9517054E-6	12.291607	3
CGGACAT	125	1.4097168E-9	12.159642	5
TCCAACG	95	1.0421372E-6	11.99755	18
CGGACCA	365	0.0	11.975181	9
GCGTTAT	135	3.7471182E-10	11.956343	1
GGTTCTA	265	0.0	11.831493	13
GTCCTAT	210	0.0	11.755396	1
CGGTCCA	260	0.0	11.695233	10
GTGCGCA	65	8.008265E-4	11.694825	9
>>END_MODULE