FastQCFastQC Report
Thu 2 Feb 2017
SRR4062131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433197
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16990.11854615939050947No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15240.10633569565105146No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14930.10417269921720461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15600.026.51651
GGTATCA7450.021.8553831
TAGGACG2150.021.4876044
ACGCGAA550.004484936619.99928711
ACCGCGT550.004484936619.9992878
AGCGAAA6400.017.87436317
ATCGATT1254.854064E-717.59998529
ATGACCG1002.4842975E-517.5993715
AGAGCGA6700.017.40236515
TCAACGC23400.017.3925424
GTATTAC1651.6570993E-917.335741
CGGACCA7250.016.9924989
CAACGCA23850.016.972145
ACGGACC6900.016.8979478
AAGACGG7050.016.8504645
ATCAACG24300.016.7483753
AACGCAG24200.016.7266756
GCGAAAG6850.016.70013218
ATACCGT3450.016.5791196
TACCGTC3450.016.5791197
Produced by FastQC (version 0.11.5)