##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1433197
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29993434259212	32.0	32.0	32.0	32.0	32.0
2	31.467159085596748	32.0	32.0	32.0	32.0	32.0
3	31.5870435118131	32.0	32.0	32.0	32.0	32.0
4	31.6576653453782	32.0	32.0	32.0	32.0	32.0
5	31.636819641682198	32.0	32.0	32.0	32.0	32.0
6	35.27996918776693	36.0	36.0	36.0	36.0	36.0
7	35.283084600372455	36.0	36.0	36.0	36.0	36.0
8	35.23924136039916	36.0	36.0	36.0	36.0	36.0
9	35.318803346643904	36.0	36.0	36.0	36.0	36.0
10	35.20168406715894	36.0	36.0	36.0	36.0	36.0
11	35.321837123577566	36.0	36.0	36.0	36.0	36.0
12	35.24519588025931	36.0	36.0	36.0	36.0	36.0
13	35.27953240203545	36.0	36.0	36.0	36.0	36.0
14	35.24067033352707	36.0	36.0	36.0	36.0	36.0
15	35.21780955444367	36.0	36.0	36.0	36.0	36.0
16	35.229430427219704	36.0	36.0	36.0	36.0	36.0
17	35.20900894992105	36.0	36.0	36.0	36.0	36.0
18	35.2086740343442	36.0	36.0	36.0	36.0	36.0
19	35.20030533136757	36.0	36.0	36.0	36.0	36.0
20	35.1991931325561	36.0	36.0	36.0	36.0	36.0
21	35.18680195395329	36.0	36.0	36.0	36.0	36.0
22	35.17230080721632	36.0	36.0	36.0	36.0	36.0
23	35.145077055003604	36.0	36.0	36.0	36.0	36.0
24	35.130591258563896	36.0	36.0	36.0	36.0	36.0
25	35.109981391253264	36.0	36.0	36.0	36.0	36.0
26	35.07327534177088	36.0	36.0	36.0	36.0	36.0
27	35.06138165234786	36.0	36.0	36.0	36.0	36.0
28	35.03278614175162	36.0	36.0	36.0	36.0	36.0
29	35.01583034293262	36.0	36.0	36.0	36.0	36.0
30	34.993580784776974	36.0	36.0	36.0	36.0	36.0
31	35.00495047087037	36.0	36.0	36.0	36.0	36.0
32	34.97778253792047	36.0	36.0	36.0	36.0	36.0
33	34.956555867755796	36.0	36.0	36.0	36.0	36.0
34	34.940336883205866	36.0	36.0	36.0	36.0	36.0
35	34.929863096280556	36.0	36.0	36.0	36.0	36.0
36	34.903390113152625	36.0	36.0	36.0	32.0	36.0
37	34.8963847956701	36.0	36.0	36.0	32.0	36.0
38	34.87964250553134	36.0	36.0	36.0	32.0	36.0
39	34.86589910528699	36.0	36.0	36.0	32.0	36.0
40	34.85367887317654	36.0	36.0	36.0	32.0	36.0
41	34.822959439630424	36.0	36.0	36.0	32.0	36.0
42	34.796589722138684	36.0	36.0	36.0	32.0	36.0
43	34.79637272475452	36.0	36.0	36.0	32.0	36.0
44	34.743465831982626	36.0	36.0	36.0	32.0	36.0
45	34.73540832139615	36.0	36.0	36.0	32.0	36.0
46	34.71575714992426	36.0	36.0	36.0	32.0	36.0
47	34.69505238986685	36.0	36.0	36.0	32.0	36.0
48	34.67064960364835	36.0	36.0	36.0	32.0	36.0
49	34.65113798033348	36.0	36.0	36.0	32.0	36.0
50	34.25721586076443	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	7.0
21	28.0
22	78.0
23	271.0
24	650.0
25	1550.0
26	3288.0
27	6267.0
28	10863.0
29	17131.0
30	25874.0
31	38285.0
32	58902.0
33	99708.0
34	229430.0
35	940857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06776466909514	17.864095153075645	11.900137011829921	26.168003165999288
2	16.361080002372404	19.738198158595257	37.08808250386388	26.81263933516846
3	18.668342181886434	23.1583927055082	28.32211477744418	29.851150335161194
4	12.616897746785682	15.874370376159035	35.14164486808164	36.36708700897364
5	14.76085615645034	36.57685341014069	33.11270754314135	15.549582890267628
6	35.34464232386917	34.8603401070056	16.232322724875033	13.562694844250192
7	31.079816661631305	29.963361631373775	20.001716442331375	18.955105264663548
8	28.36169765914944	33.26402441534555	18.786531090980514	19.587746834524495
9	27.15488519721992	14.208235155390362	18.496550020688012	40.14032962670171
10	16.542666500139198	26.596762343208923	30.836305127627256	26.024266029024623
11	37.60746080266704	21.09961156770493	21.812074683382676	19.480852946245353
12	24.577988929644704	23.500746931510463	28.457846339337856	23.463417799506978
13	29.51506317693939	19.219269925906907	25.06745409040069	26.198212806753013
14	23.601779797194663	19.38491358829247	24.838943983276547	32.17436263123632
15	25.296522390152926	27.116230357724724	21.857358060336438	25.729889191785915
16	26.041366491090844	25.72110168302994	23.326023294903884	24.91150853097533
17	24.329244339752314	25.938862556926928	24.82840809742136	24.903485005899398
18	25.031468219191844	24.942924604658693	25.81684798687126	24.208759189278197
19	25.8145949230985	24.87711040422217	24.970258799034607	24.338035873644724
20	25.853983582136415	24.407564916148882	24.70250035759265	25.03595114412205
21	26.64518555369569	24.20546512447347	24.44032467274213	24.709024649088715
22	26.084690381015314	24.193533757048055	24.86315558851993	24.858620273416705
23	24.59009416025035	24.242130345137962	25.464713454903205	25.703062039708485
24	24.99600538940999	24.675250369632305	25.280617033019553	25.048127207938148
25	25.149212595678172	24.285265735875914	25.207055589279857	25.35846607916606
26	24.711117065182428	25.27789348678092	25.417511807551392	24.59347764048527
27	25.285045747566095	24.722175899792838	24.923126087171912	25.06965226546915
28	24.90836791268629	24.525581976266714	25.430424853315397	25.135625257731597
29	24.725803401789157	24.7565739851115	25.377079194829427	25.14054341826991
30	24.756660354372297	24.92223597561231	25.513577480040915	24.807526189974478
31	25.42590364976542	24.592726146185985	24.712389442278333	25.268980761770266
32	25.24141982768485	24.69885318604398	24.54367466096562	25.516052325305544
33	24.864515993985467	24.455709097505924	25.067070426564424	25.61270448194419
34	25.36877602155197	24.69907974325751	25.296838444669117	24.635305790521407
35	25.83286003151004	24.43172382803724	25.350859688220854	24.384556452231866
36	24.67933533120057	24.978526997373002	25.061279169966404	25.280858501460024
37	25.71203696403166	24.773771836974777	24.78779662246882	24.72639457652474
38	25.03152043996743	24.552032972438543	25.17183611185343	25.244610475740597
39	25.631196400200672	24.471267263051473	24.61688637453068	25.28064996221718
40	25.71998328208413	24.716071038583078	25.04066095773563	24.52328472159716
41	24.7626948818568	25.090568080961447	25.3432237213015	24.803513315880256
42	25.900545099823326	25.075638531551874	24.94292221512045	24.080894153504353
43	25.38412265130455	24.20516964196001	25.18525806343571	25.22544964329973
44	24.85599711401121	24.94454449732788	24.867789476328404	25.331668912332507
45	25.074677062262634	25.10126146845003	24.84085988343331	24.983201585854026
46	24.877177778754632	24.85778020596315	24.934533191828738	25.330508823453478
47	25.184205752243262	24.79200100475865	25.223279699688806	24.800513543309286
48	25.870306214653787	25.15656126909699	24.22636927875407	24.74676323749516
49	25.054111572410427	25.384223563479047	24.464780378885674	25.09688448522485
50	25.221624555965917	25.38748101233143	24.477887085036148	24.913007346666507
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	122.5
2	44.0
3	136.5
4	229.0
5	238.0
6	247.0
7	194.5
8	142.0
9	190.5
10	239.0
11	447.5
12	656.0
13	1178.5
14	1701.0
15	2071.0
16	2441.0
17	2505.5
18	2570.0
19	2566.5
20	2563.0
21	3146.0
22	3729.0
23	4479.5
24	5230.0
25	6736.5
26	8243.0
27	10746.5
28	13250.0
29	15114.5
30	16979.0
31	19286.0
32	21593.0
33	25054.5
34	28516.0
35	33180.0
36	37844.0
37	44347.0
38	50850.0
39	56010.0
40	61170.0
41	66545.5
42	71921.0
43	74686.5
44	77452.0
45	84256.0
46	91060.0
47	97662.5
48	104265.0
49	108367.5
50	112470.0
51	109854.5
52	107239.0
53	107025.0
54	106811.0
55	107487.0
56	108163.0
57	104428.0
58	100693.0
59	92521.0
60	84349.0
61	74047.0
62	63745.0
63	54858.0
64	45971.0
65	38795.0
66	31619.0
67	27270.0
68	22921.0
69	20257.0
70	17593.0
71	13791.5
72	9990.0
73	8793.0
74	7596.0
75	5602.5
76	3609.0
77	3161.0
78	2713.0
79	2370.0
80	2027.0
81	1574.5
82	1122.0
83	933.5
84	745.0
85	578.5
86	412.0
87	279.5
88	147.0
89	96.5
90	46.0
91	37.5
92	29.0
93	22.0
94	15.0
95	19.0
96	23.0
97	16.0
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03307291321430341
2	0.0036282520825818084
3	4.186444710671318E-4
4	0.0
5	6.977407851118863E-5
6	6.977407851118863E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.58192628089509E-4
17	0.0
18	3.4887039255594314E-4
19	0.0
20	1.3954815702237725E-4
21	0.0
22	0.0
23	1.3954815702237725E-4
24	0.001116385256179018
25	4.884185495783204E-4
26	2.790963140447545E-4
27	4.186444710671318E-4
28	0.0013954815702237726
29	6.977407851118863E-4
30	0.0010466111776678295
31	6.279667066006976E-4
32	6.279667066006976E-4
33	1.3954815702237725E-4
34	6.977407851118863E-4
35	2.093222355335659E-4
36	1.3954815702237725E-4
37	0.0013954815702237726
38	0.0
39	4.186444710671318E-4
40	2.790963140447545E-4
41	0.001465255648734961
42	0.00453531510322726
43	0.004256218789182506
44	0.004605089181738449
45	0.0018141260412909042
46	0.0019536741983132815
47	0.0011861593346902065
48	0.0036282520825818084
49	0.003279381690025865
50	0.002093222355335659
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1433197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.83930559576648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28142492335022	40.53099642133004
2	14.610745205422976	15.732647521932948
3	4.569151785331234	7.379998778516709
4	1.8812123447993978	4.051326652887329
5	0.9857456574900009	2.6535930846651956
6	0.6040951006607055	1.9514436440026226
7	0.3720702177208725	1.4022401508470197
8	0.27662249389332416	1.1914530386708584
9	0.20577719138798065	0.9971010982598415
>10	1.0596945227364758	10.178835500771509
>50	0.0789891134098446	2.945468076364036
>100	0.06639943416178651	7.318626959732205
>500	0.006379491485820038	2.479273804629323
>1k	0.001692518149299194	1.1869952673902446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1699	0.11854615939050947	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1524	0.10633569565105146	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1493	0.10417269921720461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3954815702237725E-4
2	0.0	0.0	0.0	0.0	1.3954815702237725E-4
3	0.0	0.0	0.0	0.0	1.3954815702237725E-4
4	0.0	0.0	0.0	0.0	1.3954815702237725E-4
5	0.0	0.0	0.0	0.0	1.3954815702237725E-4
6	0.0	0.0	0.0	0.0	2.0932223553356586E-4
7	0.0	0.0	0.0	6.977407851118863E-5	3.488703925559431E-4
8	0.0	0.0	0.0	6.977407851118863E-5	3.488703925559431E-4
9	0.0	0.0	0.0	6.977407851118863E-5	4.186444710671317E-4
10	0.0	0.0	0.0	6.977407851118863E-5	4.186444710671317E-4
11	0.0	0.0	0.0	6.977407851118863E-5	4.186444710671317E-4
12	0.0	0.0	0.0	6.977407851118863E-5	6.977407851118862E-4
13	0.0	0.0	0.0	6.977407851118863E-5	7.675148636230748E-4
14	0.0	0.0	0.0	6.977407851118863E-5	7.675148636230748E-4
15	0.0	0.0	0.0	1.3954815702237725E-4	7.675148636230748E-4
16	0.0	0.0	0.0	1.3954815702237725E-4	7.675148636230748E-4
17	0.0	0.0	0.0	2.0932223553356586E-4	9.070630206454521E-4
18	0.0	0.0	0.0	2.0932223553356586E-4	9.070630206454521E-4
19	0.0	0.0	0.0	2.0932223553356586E-4	9.070630206454521E-4
20	0.0	0.0	0.0	4.186444710671317E-4	9.070630206454521E-4
21	0.0	0.0	0.0	5.58192628089509E-4	9.768370991566407E-4
22	0.0	0.0	0.0	7.675148636230748E-4	9.768370991566407E-4
23	0.0	0.0	0.0	9.768370991566407E-4	9.768370991566407E-4
24	0.0	0.0	0.0	0.0010466111776678293	9.768370991566407E-4
25	0.0	0.0	0.0	0.0013954815702237723	9.768370991566407E-4
26	0.0	0.0	0.0	0.0015350297272461496	0.0012559334132013953
27	0.0	0.0	0.0	0.0017443519627797156	0.0012559334132013953
28	0.0	0.0	0.0	0.002372318669380413	0.0012559334132013953
29	0.0	0.0	0.0	0.0032096076115146767	0.0012559334132013953
30	0.0	0.0	0.0	0.004674863260249637	0.0012559334132013953
31	0.0	0.0	0.0	0.008442663499853823	0.0012559334132013953
32	0.0	0.0	0.0	0.01214068966094682	0.0013257074917125838
33	0.0	0.0	0.0	0.01521074911543912	0.0013257074917125838
34	0.0	0.0	0.0	0.018420356726953795	0.0013954815702237723
35	0.0	0.0	0.0	0.023095219987203434	0.0013954815702237723
36	0.0	0.0	0.0	0.029235338896188033	0.0013954815702237723
37	0.0	0.0	0.0	0.03886416173073206	0.0013954815702237723
38	0.0	0.0	0.0	0.05163281809827958	0.001465255648734961
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1560	0.0	26.5165	1
GGTATCA	745	0.0	21.855383	1
TAGGACG	215	0.0	21.487604	4
ACGCGAA	55	0.0044849366	19.999287	11
ACCGCGT	55	0.0044849366	19.999287	8
AGCGAAA	640	0.0	17.874363	17
ATCGATT	125	4.854064E-7	17.599985	29
ATGACCG	100	2.4842975E-5	17.599371	5
AGAGCGA	670	0.0	17.402365	15
TCAACGC	2340	0.0	17.392542	4
GTATTAC	165	1.6570993E-9	17.33574	1
CGGACCA	725	0.0	16.992498	9
CAACGCA	2385	0.0	16.97214	5
ACGGACC	690	0.0	16.897947	8
AAGACGG	705	0.0	16.850464	5
ATCAACG	2430	0.0	16.748375	3
AACGCAG	2420	0.0	16.726675	6
GCGAAAG	685	0.0	16.700132	18
ATACCGT	345	0.0	16.579119	6
TACCGTC	345	0.0	16.579119	7
>>END_MODULE