FastQCFastQC Report
Thu 2 Feb 2017
SRR4062130_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062130_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133721
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18110.1597394773493655No Hit
TATCAACGCAGAGTACTTTTTTTTT13900.1226051206601977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG508.700427E-515.2020855
TCGAACG704.4437547E-714.9253443
ACCGATC456.748776E-414.7811118
ATTCCGA954.8803486E-914.0031586
GGTATCA5000.013.863331
CGAACGT903.639252E-813.7210744
GTCTTAC500.001504552813.2936041
CGAACGA655.45648E-513.15216516
AACGTCT801.992983E-613.0654456
CGTCGTA1501.8189894E-1212.66952210
CGGTCCA1501.8189894E-1212.66952210
TCTATAC604.0979678E-412.6639293
GCGTTAT905.432212E-712.6605761
CGTTATT853.9560327E-612.2898332
CCGACCA1551.8189894E-1212.2613719
ACCTCGC550.003062384712.0936368
AGGGTTA550.003064411512.0925675
CGTAAAC550.003072530512.0882973
AAGACGG2450.012.0220575
ACGTCTG951.0361073E-612.0027077
Produced by FastQC (version 0.11.5)