##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062130_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1133721
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168522943475512	32.0	32.0	32.0	32.0	32.0
2	30.632093786742946	32.0	32.0	32.0	32.0	32.0
3	30.65723842109302	32.0	32.0	32.0	32.0	32.0
4	30.66501105651214	32.0	32.0	32.0	32.0	32.0
5	30.567308888165606	32.0	32.0	32.0	32.0	32.0
6	34.19575274692804	36.0	36.0	36.0	32.0	36.0
7	34.13536310961868	36.0	36.0	36.0	32.0	36.0
8	34.07666965682033	36.0	36.0	36.0	32.0	36.0
9	34.254261851019784	36.0	36.0	36.0	32.0	36.0
10	33.8787223664376	36.0	36.0	36.0	32.0	36.0
11	34.238813605816595	36.0	36.0	36.0	32.0	36.0
12	34.03266941337419	36.0	36.0	36.0	32.0	36.0
13	34.12784450495316	36.0	36.0	36.0	32.0	36.0
14	34.024349906193855	36.0	36.0	36.0	32.0	36.0
15	33.96309938688619	36.0	36.0	36.0	32.0	36.0
16	33.974529006695654	36.0	36.0	36.0	32.0	36.0
17	33.905581708374456	36.0	36.0	36.0	32.0	36.0
18	33.906838631374036	36.0	36.0	36.0	32.0	36.0
19	33.912676046399426	36.0	36.0	36.0	32.0	36.0
20	33.90379026233086	36.0	36.0	36.0	32.0	36.0
21	33.88689280696044	36.0	36.0	36.0	32.0	36.0
22	33.85374973207694	36.0	36.0	36.0	32.0	36.0
23	33.825359149208666	36.0	36.0	36.0	32.0	36.0
24	33.79061867955167	36.0	36.0	36.0	32.0	36.0
25	33.42712713268961	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	23.0
5	86.0
6	250.0
7	60.0
8	201.0
9	146.0
10	84.0
11	37.0
12	48.0
13	61.0
14	154.0
15	214.0
16	406.0
17	477.0
18	695.0
19	1077.0
20	1564.0
21	2309.0
22	3581.0
23	5310.0
24	7639.0
25	10932.0
26	15042.0
27	19507.0
28	26029.0
29	34183.0
30	44381.0
31	60391.0
32	83808.0
33	116098.0
34	240014.0
35	458914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.923569519542035	17.425179278651505	11.980488837533407	25.670762364273052
2	16.983749941564003	19.061601684367997	37.28957528276407	26.66507309130393
3	19.02493755027732	22.502540327702274	28.336208843162993	30.13631327885742
4	13.171867295099826	15.315191339548859	34.65027306497014	36.862668300381166
5	15.269107340285762	35.97939154043735	33.158302016816066	15.593199102460819
6	35.33444838192915	34.33134595780174	16.30012753919955	14.034078121069566
7	31.020704076352352	30.038483478948883	19.85387227864935	19.086940166049413
8	28.400157180638523	33.186219319972984	18.67738673942894	19.736236759959557
9	26.808335511767073	14.765983227237722	18.609270236471925	39.816411024523276
10	16.80260031620693	26.562177058215642	30.509464126412112	26.12575849916532
11	37.03179256874874	21.378958833344964	21.61631437286285	19.97293422504344
12	24.21529416752639	23.708909820658857	28.36609123927698	23.70970477253777
13	29.411395831732495	19.462189802167597	25.042549113644775	26.08386525245513
14	23.5692510403199	19.522865842888734	24.750075948651645	32.15780716813972
15	25.209558518277742	27.090417986436332	21.880824136901555	25.81919935838437
16	25.87615527618442	25.848777493696485	23.324104371172076	24.950962858947015
17	24.215936941114126	26.298469894681066	24.738778123689034	24.746815040515777
18	24.913503059845816	25.18743211381037	25.561766497999844	24.337298328343973
19	25.54393604678883	25.185550134295386	25.06052563267166	24.20998818624413
20	25.66834626954227	24.54368630304015	24.70322944634909	25.08473798106849
21	26.50730376511409	24.21203653427627	24.501008709778525	24.779650990831115
22	26.01565928559256	24.148977784370913	24.83251522528656	25.00284770474997
23	24.66859588472716	24.390601329853915	25.327961184933027	25.612841600485897
24	24.765084020592614	24.828652377324108	25.182692808564777	25.2235707935185
25	25.04277458280435	24.43360095772688	25.058048066447597	25.465576393021166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	160.0
2	241.5
3	323.0
4	323.0
5	323.0
6	778.0
7	1233.0
8	1233.0
9	1233.0
10	1456.0
11	1679.0
12	1679.0
13	1679.0
14	2391.5
15	3104.0
16	3104.0
17	3104.0
18	5215.0
19	7326.0
20	7326.0
21	7326.0
22	11313.0
23	15300.0
24	15300.0
25	15300.0
26	22267.5
27	29235.0
28	29235.0
29	29235.0
30	36521.5
31	43808.0
32	43808.0
33	43808.0
34	55134.0
35	66460.0
36	66460.0
37	66460.0
38	78504.0
39	90548.0
40	90548.0
41	90548.0
42	104304.0
43	118060.0
44	118060.0
45	118060.0
46	131102.0
47	144144.0
48	144144.0
49	144144.0
50	150035.5
51	155927.0
52	155927.0
53	155927.0
54	147150.0
55	138373.0
56	138373.0
57	138373.0
58	127352.5
59	116332.0
60	116332.0
61	116332.0
62	101912.5
63	87493.0
64	87493.0
65	87493.0
66	71710.0
67	55927.0
68	55927.0
69	55927.0
70	42439.0
71	28951.0
72	28951.0
73	28951.0
74	21954.0
75	14957.0
76	14957.0
77	14957.0
78	11701.5
79	8446.0
80	8446.0
81	8446.0
82	5877.5
83	3309.0
84	3309.0
85	3309.0
86	2402.5
87	1496.0
88	1496.0
89	1496.0
90	1029.0
91	562.0
92	562.0
93	562.0
94	351.5
95	141.0
96	141.0
97	141.0
98	284.0
99	427.0
100	427.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.702563505483272E-4
2	1.764102455542413E-4
3	7.938461049940858E-4
4	0.00952615325992903
5	0.03528204911084826
6	0.0649189703639608
7	0.11351999301415427
8	0.11166768543583473
9	0.124281017992963
10	0.13680614542731412
11	0.14677332430112877
12	0.13909947861951927
13	0.13230768416568095
14	0.12145845406409514
15	0.1392758888650735
16	0.12489845385240284
17	0.12754460753571648
18	0.11563691596080518
19	0.10081845533424891
20	0.09808409652815817
21	0.09579076333595302
22	0.10849230101585841
23	0.08511794347992142
24	0.09543794284484454
25	0.09164512256542835
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1133721.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.08274273537314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.93927581718722	49.43992961928656
2	11.68156532648727	14.27084099168554
3	3.265134122750198	5.983300428493158
4	1.3559558004759338	3.3130199728403364
5	0.6798224325013639	2.076270937510819
6	0.45325413570395284	1.6611603458969073
7	0.3179085060926255	1.3593106443729854
8	0.2068837249730884	1.0109620278933478
9	0.16715188016806173	0.918908576463567
>10	0.8195041692381573	8.898208253174126
>50	0.05729975503317047	2.4489148338458095
>100	0.050880827971401164	6.183825167495987
>500	0.004928622924231869	2.054869317246722
>1k	4.3487849331457667E-4	0.38047888379407785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1811	0.1597394773493655	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1390	0.1226051206601977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.820512277712065E-5	0.0
10	0.0	0.0	0.0	8.820512277712065E-5	0.0
11	0.0	0.0	0.0	8.820512277712065E-5	0.0
12	0.0	0.0	0.0	8.820512277712065E-5	8.820512277712065E-5
13	0.0	0.0	0.0	8.820512277712065E-5	8.820512277712065E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	50	8.700427E-5	15.202085	5
TCGAACG	70	4.4437547E-7	14.925344	3
ACCGATC	45	6.748776E-4	14.781111	8
ATTCCGA	95	4.8803486E-9	14.003158	6
GGTATCA	500	0.0	13.86333	1
CGAACGT	90	3.639252E-8	13.721074	4
GTCTTAC	50	0.0015045528	13.293604	1
CGAACGA	65	5.45648E-5	13.152165	16
AACGTCT	80	1.992983E-6	13.065445	6
CGTCGTA	150	1.8189894E-12	12.669522	10
CGGTCCA	150	1.8189894E-12	12.669522	10
TCTATAC	60	4.0979678E-4	12.663929	3
GCGTTAT	90	5.432212E-7	12.660576	1
CGTTATT	85	3.9560327E-6	12.289833	2
CCGACCA	155	1.8189894E-12	12.261371	9
ACCTCGC	55	0.0030623847	12.093636	8
AGGGTTA	55	0.0030644115	12.092567	5
CGTAAAC	55	0.0030725305	12.088297	3
AAGACGG	245	0.0	12.022057	5
ACGTCTG	95	1.0361073E-6	12.002707	7
>>END_MODULE