FastQCFastQC Report
Thu 2 Feb 2017
SRR4062130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133721
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.028.6694011
GTATCAA13000.027.0852871
TATAGGA1407.2759576E-1222.001942
TAGGACG803.6165984E-621.9990314
CGTCTTA550.004483442820.015
TACTACG600.007413712318.3325237
TCAACGC19400.017.8033374
GTATAGG1751.9099389E-1017.6054361
TCTAGAT2500.017.6015532
TCTATAC1751.9099389E-1017.5992243
ATCAACG19600.017.5094323
TCTAGCG2400.017.41666628
CAACGCA20050.017.3358945
TCGTTTA2201.8189894E-1216.9992530
CGCAATA2600.016.92307936
AACGCAG20650.016.8321886
TATCAAC20900.016.5277752
TATAACC1206.408518E-616.4992713
GGCGTTA3100.016.3225842
CGAGCCG3550.016.11267715
Produced by FastQC (version 0.11.5)