##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1133721
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252832045979567	32.0	32.0	32.0	32.0	32.0
2	31.39603835511559	32.0	32.0	32.0	32.0	32.0
3	31.532206777505223	32.0	32.0	32.0	32.0	32.0
4	31.615722915955512	32.0	32.0	32.0	32.0	32.0
5	31.588208209956417	32.0	32.0	32.0	32.0	32.0
6	35.21656121744239	36.0	36.0	36.0	36.0	36.0
7	35.21447604833993	36.0	36.0	36.0	36.0	36.0
8	35.16391951811777	36.0	36.0	36.0	36.0	36.0
9	35.265151655477844	36.0	36.0	36.0	36.0	36.0
10	35.13508879168684	36.0	36.0	36.0	36.0	36.0
11	35.260781973695465	36.0	36.0	36.0	36.0	36.0
12	35.17365559956991	36.0	36.0	36.0	36.0	36.0
13	35.21256552538058	36.0	36.0	36.0	36.0	36.0
14	35.19001588574261	36.0	36.0	36.0	36.0	36.0
15	35.15723445186249	36.0	36.0	36.0	36.0	36.0
16	35.1683606460496	36.0	36.0	36.0	36.0	36.0
17	35.14198025792942	36.0	36.0	36.0	36.0	36.0
18	35.14397810396032	36.0	36.0	36.0	36.0	36.0
19	35.13845117096711	36.0	36.0	36.0	36.0	36.0
20	35.1329180636153	36.0	36.0	36.0	36.0	36.0
21	35.1278586177728	36.0	36.0	36.0	36.0	36.0
22	35.112218967453195	36.0	36.0	36.0	36.0	36.0
23	35.07572321585293	36.0	36.0	36.0	36.0	36.0
24	35.065449083151854	36.0	36.0	36.0	36.0	36.0
25	35.05199250962097	36.0	36.0	36.0	36.0	36.0
26	35.00323271774978	36.0	36.0	36.0	36.0	36.0
27	34.99534629772228	36.0	36.0	36.0	36.0	36.0
28	34.97096728383791	36.0	36.0	36.0	36.0	36.0
29	34.95009795178884	36.0	36.0	36.0	36.0	36.0
30	34.93763544999167	36.0	36.0	36.0	32.0	36.0
31	34.93822554226304	36.0	36.0	36.0	32.0	36.0
32	34.91150997467631	36.0	36.0	36.0	32.0	36.0
33	34.88735235565011	36.0	36.0	36.0	32.0	36.0
34	34.88120269449009	36.0	36.0	36.0	32.0	36.0
35	34.87067894129155	36.0	36.0	36.0	32.0	36.0
36	34.84624171202615	36.0	36.0	36.0	32.0	36.0
37	34.840932645686195	36.0	36.0	36.0	32.0	36.0
38	34.82227726221883	36.0	36.0	36.0	32.0	36.0
39	34.80647531447332	36.0	36.0	36.0	32.0	36.0
40	34.789256792455994	36.0	36.0	36.0	32.0	36.0
41	34.75724450724649	36.0	36.0	36.0	32.0	36.0
42	34.7354913598672	36.0	36.0	36.0	32.0	36.0
43	34.73594032394213	36.0	36.0	36.0	32.0	36.0
44	34.683438870762735	36.0	36.0	36.0	32.0	36.0
45	34.67788283007901	36.0	36.0	36.0	32.0	36.0
46	34.65385751873697	36.0	36.0	36.0	32.0	36.0
47	34.634256576353444	36.0	36.0	36.0	32.0	36.0
48	34.6183778901511	36.0	36.0	36.0	32.0	36.0
49	34.59590146076504	36.0	36.0	36.0	32.0	36.0
50	34.16740714867238	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	10.0
21	32.0
22	62.0
23	225.0
24	547.0
25	1207.0
26	2561.0
27	5138.0
28	8838.0
29	14452.0
30	22014.0
31	33202.0
32	50374.0
33	85811.0
34	194130.0
35	715113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.565054430399854	17.97279627025148	12.383026256116693	26.079123043231977
2	16.292288951086704	19.61992098152972	36.98598961416087	27.101800453222708
3	18.341862791276135	23.07910785574543	28.70590393731069	29.87312541566774
4	12.843018696839875	15.810856462921654	34.86960195674244	36.476522883496024
5	14.944430723635465	36.363828811346714	32.9110362346964	15.780704230321419
6	35.2686730409625	34.72418233779064	16.17559891331193	13.83154570793494
7	31.118679110645388	29.96857251475451	19.942031593310876	18.970716781289223
8	28.353801332073765	33.35723692160593	18.56488501139169	19.724076734928612
9	26.95945475121304	14.456378597556188	18.577674754194373	40.0064918970364
10	16.777496403436118	26.579378877166427	30.610529398326396	26.032595321071057
11	37.20403873616172	21.261139204442717	21.64730123196095	19.887520827434617
12	24.31268363203998	23.57017290850218	28.385819791641858	23.73132366781598
13	29.449749982579487	19.329447015623774	25.07795127725428	26.14285172454246
14	23.574935985132146	19.314275734506108	24.81360052429125	32.29718775607049
15	25.267680496347865	27.107374742110274	21.787194556685463	25.8377502048564
16	25.999610131981793	25.766659580830137	23.228018985337613	25.005711301850464
17	24.348406706764717	26.17090095358558	24.729452837161876	24.751239502487827
18	24.99940461437093	25.212861388051184	25.572474308007543	24.215259689570342
19	25.752103030639812	24.999977948719305	25.096827173528585	24.151091847112298
20	26.002455641448126	24.298148742718634	24.688634543395345	25.010761072437894
21	26.651354257352562	24.185580050118148	24.49041695443588	24.67264873809341
22	26.032507115948277	24.11307543919536	24.839444625264946	25.01497281959142
23	24.771041148644418	24.213319173445978	25.38001039058179	25.63562928732781
24	24.887206306391334	24.811437170274154	25.098724802792958	25.20263172054155
25	25.101369916178523	24.354800439967928	25.112660191811198	25.43116945204235
26	24.84019408689455	25.10260479007585	25.344375458116165	24.71282566491344
27	25.349580802935485	24.64861098247796	24.901937435775302	25.09987077881125
28	24.997530210814148	24.632266031578016	25.28614271853224	25.084061039075596
29	24.91814528506257	24.915499119708993	25.22324815033042	24.943107444898015
30	24.87254078694818	24.871394095066048	25.34462501411313	24.911440103872643
31	25.375203864809297	24.530901450714772	24.73518523582164	25.35870944865429
32	25.197426857461913	24.726671964901108	24.605388851303374	25.470512326333605
33	24.79602511382901	24.4708119655858	25.099627949807623	25.633534970777568
34	25.491130022439563	24.48090873167083	25.20560777340277	24.82235347248684
35	25.76288677980454	24.46653494690047	25.16450269907914	24.606075574215854
36	24.707576934984658	24.95516958817765	24.944673141533556	25.39258033530414
37	25.698418634846487	24.682368590037612	24.807357123199704	24.811855651916197
38	24.937286102388597	24.48444060261793	25.27634689341284	25.301926401580637
39	25.611309377850144	24.543315157085473	24.616437655352232	25.22893780971215
40	25.651130790671417	24.667289983417422	24.993208199555443	24.688371026355714
41	24.771810730805463	25.103024764754238	25.27458569578763	24.850578808652667
42	25.73957424587111	24.958298747683386	24.816368943394796	24.485758063050707
43	25.330296609795727	24.338304174553972	25.00172006513313	25.329679150517165
44	25.036805340545325	24.777028880614854	24.80728467883426	25.378881100005557
45	25.177142000273445	25.031776624224328	24.77429996604025	25.01678140946198
46	25.061678846603364	24.822902103299388	24.834280825089643	25.28113822500761
47	25.114491562201202	24.803741351388542	25.046925663398333	25.034841423011923
48	25.741373394268013	25.241140963928753	24.289190974822546	24.72829466698069
49	25.15709900501023	25.23339919554019	24.380160186295956	25.229341613153622
50	25.144261386990983	25.289184321155574	24.596676739824964	24.96987755202848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	59.0
2	36.0
3	91.5
4	147.0
5	160.5
6	174.0
7	138.0
8	102.0
9	140.0
10	178.0
11	307.5
12	437.0
13	880.5
14	1324.0
15	1617.0
16	1910.0
17	1930.0
18	1950.0
19	1924.0
20	1898.0
21	2403.0
22	2908.0
23	3670.5
24	4433.0
25	5522.5
26	6612.0
27	8334.0
28	10056.0
29	11696.0
30	13336.0
31	15183.5
32	17031.0
33	19491.5
34	21952.0
35	25298.5
36	28645.0
37	33788.0
38	38931.0
39	43324.0
40	47717.0
41	52424.5
42	57132.0
43	59961.5
44	62791.0
45	67853.0
46	72915.0
47	77634.0
48	82353.0
49	85739.0
50	89125.0
51	87792.5
52	86460.0
53	85841.0
54	85222.0
55	85084.5
56	84947.0
57	81988.0
58	79029.0
59	73091.0
60	67153.0
61	58633.0
62	50113.0
63	43559.0
64	37005.0
65	30932.5
66	24860.0
67	21675.5
68	18491.0
69	16277.5
70	14064.0
71	10974.0
72	7884.0
73	6909.0
74	5934.0
75	4407.5
76	2881.0
77	2536.5
78	2192.0
79	1841.0
80	1490.0
81	1164.5
82	839.0
83	666.0
84	493.0
85	402.5
86	312.0
87	198.0
88	84.0
89	55.5
90	27.0
91	22.5
92	18.0
93	15.0
94	12.0
95	14.0
96	16.0
97	14.5
98	13.0
99	10.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030607177603660867
2	0.0054687176121814805
3	2.6461536833136194E-4
4	0.0
5	8.820512277712065E-5
6	8.820512277712065E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.528204911084826E-4
17	0.0
18	1.764102455542413E-4
19	0.0
20	4.4102561388560327E-4
21	0.0
22	0.0
23	1.764102455542413E-4
24	0.001234871718879689
25	1.764102455542413E-4
26	1.764102455542413E-4
27	5.292307366627239E-4
28	0.0018523075783195338
29	4.4102561388560327E-4
30	0.002205128069428016
31	3.528204911084826E-4
32	8.820512277712065E-4
33	2.6461536833136194E-4
34	7.938461049940858E-4
35	8.820512277712065E-5
36	3.528204911084826E-4
37	0.001499487087211051
38	8.820512277712065E-5
39	6.174358594398445E-4
40	8.820512277712065E-5
41	0.0011466665961025685
42	0.0054687176121814805
43	0.0037928202794161882
44	0.004763076629964515
45	0.002293333192205137
46	0.002293333192205137
47	0.0011466665961025685
48	0.004233845893301791
49	0.0036164100338619467
50	0.0013230768416568099
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1133721.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.075514596755795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49851266917696	49.96986598212626
2	12.117541010742482	15.044051877782639
3	3.3185547137455202	6.180029747197284
4	1.3396387961657688	3.326350705830741
5	0.7084245675737401	2.1987909792562053
6	0.4577137434824956	1.7047689698810036
7	0.29098873579601126	1.2644292861477768
8	0.22733280060528166	1.1289440465835627
9	0.15661290664464442	0.874964409521398
>10	0.782807372836115	8.533301455259657
>50	0.05302843085192444	2.2981532919432976
>100	0.04441685316176307	5.56439753705578
>500	0.004284579887637339	1.8199269559453082
>1k	1.4281932958791127E-4	0.09202475546917527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	8.820512277712065E-5	0.0	0.0	8.820512277712065E-5
14	0.0	8.820512277712065E-5	0.0	0.0	8.820512277712065E-5
15	0.0	8.820512277712065E-5	0.0	0.0	8.820512277712065E-5
16	0.0	8.820512277712065E-5	0.0	0.0	8.820512277712065E-5
17	0.0	8.820512277712065E-5	0.0	0.0	8.820512277712065E-5
18	0.0	8.820512277712065E-5	0.0	0.0	8.820512277712065E-5
19	0.0	8.820512277712065E-5	0.0	0.0	8.820512277712065E-5
20	0.0	8.820512277712065E-5	0.0	0.0	1.764102455542413E-4
21	0.0	8.820512277712065E-5	0.0	0.0	1.764102455542413E-4
22	0.0	8.820512277712065E-5	0.0	0.0	1.764102455542413E-4
23	0.0	8.820512277712065E-5	0.0	8.820512277712065E-5	1.764102455542413E-4
24	0.0	8.820512277712065E-5	0.0	4.4102561388560327E-4	1.764102455542413E-4
25	0.0	8.820512277712065E-5	0.0	7.938461049940859E-4	1.764102455542413E-4
26	0.0	8.820512277712065E-5	0.0	9.702563505483272E-4	1.764102455542413E-4
27	0.0	8.820512277712065E-5	0.0	0.001234871718879689	1.764102455542413E-4
28	0.0	8.820512277712065E-5	0.0	0.0013230768416568096	1.764102455542413E-4
29	0.0	8.820512277712065E-5	0.0	0.0019405127010966544	1.764102455542413E-4
30	0.0	8.820512277712065E-5	0.0	0.0031753844199763435	1.764102455542413E-4
31	0.0	8.820512277712065E-5	0.0	0.0052923073666272386	1.764102455542413E-4
32	0.0	8.820512277712065E-5	0.0	0.007673845681609497	1.764102455542413E-4
33	0.0	8.820512277712065E-5	0.0	0.010408204487700237	2.6461536833136194E-4
34	0.0	8.820512277712065E-5	0.0	0.01358358890767658	3.528204911084826E-4
35	8.820512277712065E-5	8.820512277712065E-5	0.0	0.018170255292086854	3.528204911084826E-4
36	8.820512277712065E-5	8.820512277712065E-5	0.0	0.0243446138864853	3.528204911084826E-4
37	8.820512277712065E-5	8.820512277712065E-5	0.0	0.03157743395420919	3.528204911084826E-4
38	8.820512277712065E-5	8.820512277712065E-5	0.0	0.042955894792457754	3.528204911084826E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	545	0.0	28.669401	1
GTATCAA	1300	0.0	27.085287	1
TATAGGA	140	7.2759576E-12	22.00194	2
TAGGACG	80	3.6165984E-6	21.999031	4
CGTCTTA	55	0.0044834428	20.0	15
TACTACG	60	0.0074137123	18.332523	7
TCAACGC	1940	0.0	17.803337	4
GTATAGG	175	1.9099389E-10	17.605436	1
TCTAGAT	250	0.0	17.601553	2
TCTATAC	175	1.9099389E-10	17.599224	3
ATCAACG	1960	0.0	17.509432	3
TCTAGCG	240	0.0	17.416666	28
CAACGCA	2005	0.0	17.335894	5
TCGTTTA	220	1.8189894E-12	16.99925	30
CGCAATA	260	0.0	16.923079	36
AACGCAG	2065	0.0	16.832188	6
TATCAAC	2090	0.0	16.527775	2
TATAACC	120	6.408518E-6	16.499271	3
GGCGTTA	310	0.0	16.32258	42
CGAGCCG	355	0.0	16.112677	15
>>END_MODULE