FastQCFastQC Report
Thu 2 Feb 2017
SRR4062129_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062129_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219907
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33540.27493899125097243No Hit
TATCAACGCAGAGTACTTTTTTTTT20990.17206229655211422No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13140.1077131289516332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTA500.00149836813.30100512
GTCTAAC500.001504405813.293911
GACGGAC604.0868702E-412.6681457
TAAGACC1251.4115358E-912.1589234
GCGTACT550.003066052412.09182514
AACCAGT1103.8086E-812.0913286
GCCTTAT550.003078331512.0853741
TTATAAG802.8725892E-511.8719992
GTGTTAC1157.111885E-811.5599221
CTATACT1001.9303152E-611.398994
CTAAGAC1354.7366484E-911.2568753
CTTATAC855.331922E-511.1741053
GTATCAA14850.010.9343851
GTCTTAG1855.456968E-1210.7788461
GGTATCA6700.010.7711841
CAAGACG803.7724985E-410.6865544
GGTTAAG803.7853987E-410.6826071
CTAATAC1252.24416E-710.6377483
TAGACAG1355.677066E-810.5559215
TTGGACA1803.45608E-1110.5546214
Produced by FastQC (version 0.11.5)