##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062129_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1219907
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162221382449644	32.0	32.0	32.0	32.0	32.0
2	30.730076964883388	32.0	32.0	32.0	32.0	32.0
3	30.754608343095008	32.0	32.0	32.0	32.0	32.0
4	30.752677868066993	32.0	32.0	32.0	32.0	32.0
5	30.697659739635892	32.0	32.0	32.0	32.0	32.0
6	34.31276892418848	36.0	36.0	36.0	32.0	36.0
7	34.25120685429299	36.0	36.0	36.0	32.0	36.0
8	34.19395740822866	36.0	36.0	36.0	32.0	36.0
9	34.31131471497417	36.0	36.0	36.0	32.0	36.0
10	34.03135730838498	36.0	36.0	36.0	32.0	36.0
11	34.31051957239363	36.0	36.0	36.0	32.0	36.0
12	34.14516188529126	36.0	36.0	36.0	32.0	36.0
13	34.21896095358089	36.0	36.0	36.0	32.0	36.0
14	34.12741545052205	36.0	36.0	36.0	32.0	36.0
15	34.092158664553935	36.0	36.0	36.0	32.0	36.0
16	34.0973975885047	36.0	36.0	36.0	32.0	36.0
17	34.040867049701326	36.0	36.0	36.0	32.0	36.0
18	34.040344878748954	36.0	36.0	36.0	32.0	36.0
19	34.04392629929986	36.0	36.0	36.0	32.0	36.0
20	34.024920752155694	36.0	36.0	36.0	32.0	36.0
21	34.00250920766911	36.0	36.0	36.0	32.0	36.0
22	33.97337911824426	36.0	36.0	36.0	32.0	36.0
23	33.94798292000947	36.0	36.0	36.0	32.0	36.0
24	33.92293756819167	36.0	36.0	36.0	32.0	36.0
25	33.56352492444096	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	17.0
5	96.0
6	248.0
7	64.0
8	205.0
9	183.0
10	88.0
11	28.0
12	48.0
13	66.0
14	257.0
15	346.0
16	507.0
17	671.0
18	960.0
19	1262.0
20	1797.0
21	2524.0
22	3786.0
23	5607.0
24	7761.0
25	11012.0
26	15002.0
27	19418.0
28	25562.0
29	33524.0
30	44415.0
31	59241.0
32	82869.0
33	116710.0
34	250709.0
35	534923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69525837794768	16.9047601481762	11.437668200400196	25.962313273475917
2	16.579897614978215	19.50692881822765	37.66162119181411	26.25155237498002
3	19.204048859782656	23.41464334342433	28.1567572397387	29.224550557054314
4	12.833173742236331	15.539964452722776	35.756412610757316	35.87044919428358
5	14.684210267368805	36.44066406781718	33.858159889923364	15.016965774890654
6	34.30458274308372	35.09162779290257	16.8587592495224	13.745030214491308
7	30.14240006433565	30.33417609750133	20.693840271982236	18.829583566180787
8	27.719228850168726	33.51327485917619	19.413742958809856	19.35375333184522
9	27.19666851603203	14.572780187059475	18.394975197712476	39.83557609919602
10	15.347311203939393	27.476094984728206	32.47256946183036	24.704024349502035
11	36.34000116571453	21.6097204173258	22.578659050665074	19.471619366294597
12	24.240760167386547	23.51546224198815	29.52880260174774	22.71497498887756
13	29.390582740633427	20.155873577417623	25.1128010867647	25.340742595184253
14	23.095515378927214	19.31367069554591	25.969077182295912	31.621736743230965
15	24.841818390766857	27.158066687462036	22.608560030208007	25.3915548915631
16	24.99565011851375	26.320476287400773	24.02981228209556	24.654061311989913
17	23.74070249799317	26.00156935103395	25.668082256319675	24.58964589465321
18	24.014894051817315	25.86089310144747	26.194999807135655	23.929213039599563
19	24.974788067965953	25.407062951465903	25.999020242576123	23.619128737992025
20	25.265621166657233	25.083138666187487	25.793540414868716	23.85769975228657
21	25.196573229938373	24.860411058151872	25.532078995238592	24.410936716671166
22	25.047574110165144	24.85350292337676	25.503661914042464	24.595261052415633
23	24.37750740427766	25.002994527808088	25.543650370418984	25.075847697495263
24	24.159332553288905	25.277460052808898	25.790205245771432	24.773002148130765
25	24.547865313831768	24.90314036892812	25.87395202063713	24.67504229660298
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	303.0
1	303.0
2	435.5
3	568.0
4	568.0
5	568.0
6	1443.0
7	2318.0
8	2318.0
9	2318.0
10	2543.5
11	2769.0
12	2769.0
13	2769.0
14	3464.5
15	4160.0
16	4160.0
17	4160.0
18	6978.5
19	9797.0
20	9797.0
21	9797.0
22	14402.5
23	19008.0
24	19008.0
25	19008.0
26	27063.5
27	35119.0
28	35119.0
29	35119.0
30	44765.5
31	54412.0
32	54412.0
33	54412.0
34	65074.0
35	75736.0
36	75736.0
37	75736.0
38	89001.5
39	102267.0
40	102267.0
41	102267.0
42	116293.5
43	130320.0
44	130320.0
45	130320.0
46	144005.0
47	157690.0
48	157690.0
49	157690.0
50	162952.5
51	168215.0
52	168215.0
53	168215.0
54	158819.5
55	149424.0
56	149424.0
57	149424.0
58	134787.0
59	120150.0
60	120150.0
61	120150.0
62	102875.5
63	85601.0
64	85601.0
65	85601.0
66	69237.0
67	52873.0
68	52873.0
69	52873.0
70	39815.0
71	26757.0
72	26757.0
73	26757.0
74	19671.0
75	12585.0
76	12585.0
77	12585.0
78	9162.5
79	5740.0
80	5740.0
81	5740.0
82	3988.0
83	2236.0
84	2236.0
85	2236.0
86	1580.5
87	925.0
88	925.0
89	925.0
90	604.5
91	284.0
92	284.0
93	284.0
94	198.0
95	112.0
96	112.0
97	112.0
98	325.0
99	538.0
100	538.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.557876952915263E-4
2	1.6394692382288157E-4
3	6.557876952915263E-4
4	0.010082735815107217
5	0.03221557053119623
6	0.06475903491003823
7	0.10640155356105015
8	0.1117298285852938
9	0.12058296247172941
10	0.1350922652300544
11	0.1450930275832502
12	0.13517423869196585
13	0.13214122060124256
14	0.11918941361923491
15	0.1382892302446006
16	0.1215666440146667
17	0.1286163617390506
18	0.11754994438100609
19	0.1014831458463637
20	0.09508921581727131
21	0.09426948119815691
22	0.1050899781704671
23	0.08336701076393528
24	0.09615487082212004
25	0.09287593234566241
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1219907.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.273571797902356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.70921843128062	42.95299432422499
2	14.97540710537956	17.453409223163057
3	5.099325136454075	8.914676683800142
4	2.2147691502218696	5.1625003636493325
5	1.170348804331171	3.410020253889083
6	0.6967116711764412	2.435992655564213
7	0.4399869135810283	1.7947726299091058
8	0.297924002791577	1.388887661359474
9	0.22263552509717305	1.1676390530862426
>10	1.1106393675516035	11.22597514272907
>50	0.04733233484999737	1.9073139565431638
>100	0.014711376328277598	1.325572890794492
>500	2.829088447816421E-4	0.12455255257816022
>1k	7.072721119541053E-4	0.7356926087095168
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3354	0.27493899125097243	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2099	0.17206229655211422	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1314	0.1077131289516332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6394692382288157E-4
2	0.0	0.0	0.0	0.0	1.6394692382288157E-4
3	0.0	0.0	0.0	0.0	1.6394692382288157E-4
4	0.0	0.0	0.0	0.0	1.6394692382288157E-4
5	0.0	0.0	0.0	0.0	1.6394692382288157E-4
6	0.0	0.0	0.0	0.0	1.6394692382288157E-4
7	0.0	0.0	0.0	0.0	1.6394692382288157E-4
8	0.0	0.0	0.0	0.0	2.4592038573432236E-4
9	0.0	0.0	0.0	0.0	2.4592038573432236E-4
10	0.0	0.0	0.0	0.0	3.2789384764576315E-4
11	0.0	0.0	0.0	0.0	3.2789384764576315E-4
12	0.0	0.0	0.0	0.0	7.377611572029671E-4
13	0.0	0.0	0.0	0.0	0.0010656550048487303
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGTA	50	0.001498368	13.301005	12
GTCTAAC	50	0.0015044058	13.29391	1
GACGGAC	60	4.0868702E-4	12.668145	7
TAAGACC	125	1.4115358E-9	12.158923	4
GCGTACT	55	0.0030660524	12.091825	14
AACCAGT	110	3.8086E-8	12.091328	6
GCCTTAT	55	0.0030783315	12.085374	1
TTATAAG	80	2.8725892E-5	11.871999	2
GTGTTAC	115	7.111885E-8	11.559922	1
CTATACT	100	1.9303152E-6	11.39899	4
CTAAGAC	135	4.7366484E-9	11.256875	3
CTTATAC	85	5.331922E-5	11.174105	3
GTATCAA	1485	0.0	10.934385	1
GTCTTAG	185	5.456968E-12	10.778846	1
GGTATCA	670	0.0	10.771184	1
CAAGACG	80	3.7724985E-4	10.686554	4
GGTTAAG	80	3.7853987E-4	10.682607	1
CTAATAC	125	2.24416E-7	10.637748	3
TAGACAG	135	5.677066E-8	10.555921	5
TTGGACA	180	3.45608E-11	10.554621	4
>>END_MODULE