FastQCFastQC Report
Thu 2 Feb 2017
SRR4062129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219907
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGT450.001398145124.44467242
TTAACGG952.6862836E-823.15716235
GTATCAA17900.021.5147531
TAACGGC1057.745439E-820.95257836
CGTCGTA956.8657937E-720.84229910
ATACGAT755.4740227E-520.5326846
TAGTGCG550.00448445619.9993698
GTATAGG1705.456968E-1219.4175171
GTATATA1851.8189894E-1219.0326651
CCGTCGT951.5975655E-518.5257289
CGCGGTA1254.850008E-717.60088543
ACTAGAC1154.2742395E-617.2168463
TAAGACC2600.016.922544
AATACGA1307.3193587E-716.922545
GTATTAG2350.016.8560581
CCCGACA1206.4085616E-616.49947721
TTATTCC2950.016.406264
GGTATCA10500.016.1381151
TATAGTG1501.8382707E-716.1328225
GTATTAC1653.0990122E-816.0047421
Produced by FastQC (version 0.11.5)