##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1219907
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227282899434137	32.0	32.0	32.0	32.0	32.0
2	31.38970347739623	32.0	32.0	32.0	32.0	32.0
3	31.532429111399477	32.0	32.0	32.0	32.0	32.0
4	31.615961708556473	32.0	32.0	32.0	32.0	32.0
5	31.593358346169012	32.0	32.0	32.0	32.0	32.0
6	35.21504754050923	36.0	36.0	36.0	36.0	36.0
7	35.21611319551408	36.0	36.0	36.0	36.0	36.0
8	35.17200737433264	36.0	36.0	36.0	36.0	36.0
9	35.258118856601364	36.0	36.0	36.0	36.0	36.0
10	35.137137503104746	36.0	36.0	36.0	36.0	36.0
11	35.260746925790244	36.0	36.0	36.0	36.0	36.0
12	35.18105396558918	36.0	36.0	36.0	36.0	36.0
13	35.21622467942228	36.0	36.0	36.0	36.0	36.0
14	35.180797388653396	36.0	36.0	36.0	36.0	36.0
15	35.15899572672343	36.0	36.0	36.0	36.0	36.0
16	35.17471741698343	36.0	36.0	36.0	36.0	36.0
17	35.15702016629137	36.0	36.0	36.0	36.0	36.0
18	35.15354695071018	36.0	36.0	36.0	36.0	36.0
19	35.14203869639243	36.0	36.0	36.0	36.0	36.0
20	35.137703939726556	36.0	36.0	36.0	36.0	36.0
21	35.12960742089356	36.0	36.0	36.0	36.0	36.0
22	35.10589085889334	36.0	36.0	36.0	36.0	36.0
23	35.08404984970166	36.0	36.0	36.0	36.0	36.0
24	35.0640368487106	36.0	36.0	36.0	36.0	36.0
25	35.051750666239315	36.0	36.0	36.0	36.0	36.0
26	35.01250423187997	36.0	36.0	36.0	36.0	36.0
27	34.997282579737636	36.0	36.0	36.0	36.0	36.0
28	34.978023734596164	36.0	36.0	36.0	36.0	36.0
29	34.962319258763166	36.0	36.0	36.0	36.0	36.0
30	34.9356114851378	36.0	36.0	36.0	36.0	36.0
31	34.930571756699486	36.0	36.0	36.0	36.0	36.0
32	34.9046050231698	36.0	36.0	36.0	32.0	36.0
33	34.87946950054389	36.0	36.0	36.0	32.0	36.0
34	34.86086890230157	36.0	36.0	36.0	32.0	36.0
35	34.84137561305903	36.0	36.0	36.0	32.0	36.0
36	34.81826073626924	36.0	36.0	36.0	32.0	36.0
37	34.80662542308553	36.0	36.0	36.0	32.0	36.0
38	34.7932137449822	36.0	36.0	36.0	32.0	36.0
39	34.763744285425034	36.0	36.0	36.0	32.0	36.0
40	34.758810302752586	36.0	36.0	36.0	32.0	36.0
41	34.722664924457355	36.0	36.0	36.0	32.0	36.0
42	34.708576965293254	36.0	36.0	36.0	32.0	36.0
43	34.68508419084406	36.0	36.0	36.0	32.0	36.0
44	34.65747388940304	36.0	36.0	36.0	32.0	36.0
45	34.62882990260733	36.0	36.0	36.0	32.0	36.0
46	34.604310820415	36.0	36.0	36.0	32.0	36.0
47	34.56603659131393	36.0	36.0	36.0	32.0	36.0
48	34.54563175717493	36.0	36.0	36.0	32.0	36.0
49	34.53053552442932	36.0	36.0	36.0	32.0	36.0
50	34.10907880682708	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	7.0
19	8.0
20	30.0
21	56.0
22	108.0
23	289.0
24	689.0
25	1530.0
26	3181.0
27	6091.0
28	10333.0
29	16299.0
30	24753.0
31	36332.0
32	54061.0
33	90275.0
34	201612.0
35	774252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.140917436007186	17.441419014046975	11.812790117018476	26.604873432927363
2	15.865397227213704	20.058269172754816	37.33465261473485	26.74168098529662
3	18.531898463974976	23.817241057068518	28.501797685387842	29.149062793568664
4	12.557760550599347	15.958019750685912	35.864701161645925	35.619518537068814
5	14.312320529351826	36.73517735368352	33.79249401798662	15.160008098978036
6	34.369451416748504	35.32911552201563	16.700631032227072	13.600802029008793
7	30.102130736195466	30.336656810724094	20.705430823825093	18.855781629255343
8	27.73350755426438	33.576657892773795	19.28269941889013	19.407135134071694
9	27.25879923633523	14.40511448823558	18.297460380176524	40.03862589525267
10	15.246105229505577	27.51902812104221	32.54507523126801	24.689791418184203
11	36.51032414766044	21.452291035300235	22.57631114502991	19.461073672009423
12	24.254635804204746	23.48154408491795	29.56749981760905	22.696320293268258
13	29.596600396587608	19.949963398849256	25.12322660661837	25.330209597944766
14	23.124713605217448	19.147689127121986	26.017147208762637	31.710450058897933
15	24.956000744319034	27.152397682774176	22.6213965490812	25.270205023825586
16	25.146138907993354	26.07891951887899	24.03203210752348	24.742909465604175
17	23.756892943478476	25.933616251074877	25.734994552863455	24.574496252583188
18	24.163580353175913	25.652388470549276	26.274487622776256	23.909543553498562
19	25.07387858254769	25.28873102621757	26.066577206295232	23.570813184939507
20	25.34088803854736	24.884293984270865	25.87019285155693	23.904625125624847
21	25.285062947473	24.70149339375329	25.665256175475815	24.34818748329789
22	25.137162095143317	24.736557786782107	25.569080265954703	24.557199852119872
23	24.510413097402587	24.884294363664086	25.49073453200493	25.1145580069284
24	24.380806856989214	25.048979665446343	25.865771944119736	24.704441533444708
25	24.697353234352867	24.629642988300716	25.94679581344106	24.726207963905356
26	24.40302581682791	25.034879851004426	25.993891312580846	24.56820301958682
27	24.577855780336634	25.26307419524339	25.576787662625634	24.582282361794338
28	24.64484386245928	24.938887732132347	25.716911252362927	24.699357153045447
29	24.453439702533483	25.299737192003946	25.701818670680105	24.545004434782467
30	24.689070835778907	25.13812698173281	25.69752076413322	24.47528141835507
31	24.658046302080002	24.836913129087417	25.80190867791019	24.703131890922386
32	24.45688082118341	25.26416142989004	25.770351291665367	24.50860645726118
33	24.50078038927653	25.052217223650388	25.623327737264574	24.82367464980851
34	24.528158045741456	25.111074678252315	25.876137388310518	24.484629887695714
35	24.714833884333427	25.084781330974675	25.774918169723332	24.425466614968567
36	24.640115418823598	25.123595689828303	25.784794717621455	24.451494173726644
37	25.076195394666733	24.969546434514587	25.490658993843706	24.463599176974974
38	24.682003926535263	25.152532369323843	25.624372389653296	24.541091314487602
39	24.740305730980346	25.286417858571554	25.412986025038197	24.560290385409903
40	24.825253072570284	25.128308961256884	25.60924726229131	24.437190703881527
41	24.4598702180193	25.22575769002502	25.78318408514852	24.53118800680716
42	24.735190029487548	25.326334584046883	25.51103139189908	24.427443994566485
43	24.559108157759475	25.04236152270307	25.627102193125918	24.77142812641154
44	24.467740626488403	25.343342238821755	25.617884865888918	24.571032268800924
45	24.671117348477893	25.257974502577284	25.405365082696118	24.665543066248706
46	24.71839466308599	25.265743133961426	25.33337268143368	24.682489521518903
47	24.702495374609494	25.45395225990391	25.430097786944305	24.413454578542286
48	24.660534815470626	25.565474726607974	25.228058957585297	24.545931500336103
49	24.731954968270713	25.390641971847383	25.21348918843367	24.663913871448234
50	24.536044613822057	25.687786859645655	25.16380561869872	24.612362907833568
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	113.5
2	86.0
3	299.5
4	513.0
5	471.0
6	429.0
7	358.5
8	288.0
9	378.5
10	469.0
11	691.0
12	913.0
13	1636.0
14	2359.0
15	2980.5
16	3602.0
17	3515.0
18	3428.0
19	3382.5
20	3337.0
21	3869.5
22	4402.0
23	5111.0
24	5820.0
25	7194.5
26	8569.0
27	10546.5
28	12524.0
29	14847.5
30	17171.0
31	19855.0
32	22539.0
33	25976.0
34	29413.0
35	32843.0
36	36273.0
37	40238.5
38	44204.0
39	48696.0
40	53188.0
41	58139.5
42	63091.0
43	66763.5
44	70436.0
45	75614.0
46	80792.0
47	84098.5
48	87405.0
49	89483.5
50	91562.0
51	92616.0
52	93670.0
53	93304.5
54	92939.0
55	89905.0
56	86871.0
57	82597.0
58	78323.0
59	71933.5
60	65544.0
61	58126.0
62	50708.0
63	43863.0
64	37018.0
65	31136.0
66	25254.0
67	21128.5
68	17003.0
69	13931.5
70	10860.0
71	8881.5
72	6903.0
73	5847.0
74	4791.0
75	3833.5
76	2876.0
77	2390.5
78	1905.0
79	1495.5
80	1086.0
81	855.0
82	624.0
83	462.0
84	300.0
85	230.0
86	160.0
87	98.0
88	36.0
89	30.0
90	24.0
91	18.5
92	13.0
93	12.0
94	11.0
95	15.0
96	19.0
97	14.5
98	10.0
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029182552440472923
2	0.00418064655748348
3	4.0986730955720394E-4
4	0.0
5	0.0
6	8.197346191144079E-5
7	0.0
8	0.0
9	0.0
10	1.6394692382288157E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.918407714686447E-4
17	0.0
18	4.0986730955720394E-4
19	0.0
20	4.0986730955720394E-4
21	8.197346191144079E-5
22	0.0
23	8.197346191144079E-5
24	0.0010656550048487301
25	2.4592038573432236E-4
26	4.0986730955720394E-4
27	3.2789384764576315E-4
28	0.0017214427001402566
29	4.0986730955720394E-4
30	0.001229601928671612
31	4.0986730955720394E-4
32	7.377611572029671E-4
33	2.4592038573432236E-4
34	5.738142333800855E-4
35	3.2789384764576315E-4
36	1.6394692382288157E-4
37	0.0013935488524944935
38	1.6394692382288157E-4
39	9.017080810258488E-4
40	0.0
41	0.001229601928671612
42	0.005738142333800856
43	0.00409867309557204
44	0.004918407714686448
45	0.0015574957763173749
46	0.0021313100096974603
47	8.197346191144079E-4
48	0.0038527527098377173
49	0.004590513867040684
50	0.0013115753905830526
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1219907.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.96772278196726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97210464263424	43.02998837383562
2	15.535993490917996	18.322443146298003
3	5.202549515769209	9.203474928160102
4	2.280272763881079	5.37849968831239
5	1.192767367662126	3.516738773983853
6	0.7019915016935692	2.4836904160297975
7	0.45626567077756497	1.8833463312537855
8	0.2933094494138207	1.3836632241892504
9	0.2276274634128494	1.208040584408217
>10	1.084713384451264	10.971657165140893
>50	0.04233477426024247	1.688663227373826
>100	0.009650397441298607	0.7421707914690924
>500	2.7971845649450423E-4	0.09640953653547318
>1k	1.3985922824725212E-4	0.09121381300960936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.197346191144079E-5
2	0.0	0.0	0.0	0.0	1.6394692382288157E-4
3	0.0	0.0	0.0	0.0	1.6394692382288157E-4
4	0.0	0.0	0.0	0.0	1.6394692382288157E-4
5	0.0	0.0	0.0	0.0	1.6394692382288157E-4
6	0.0	0.0	0.0	0.0	3.2789384764576315E-4
7	0.0	0.0	0.0	0.0	3.2789384764576315E-4
8	0.0	0.0	0.0	0.0	3.2789384764576315E-4
9	0.0	0.0	0.0	0.0	4.0986730955720394E-4
10	0.0	0.0	0.0	0.0	4.0986730955720394E-4
11	0.0	0.0	0.0	0.0	4.0986730955720394E-4
12	0.0	0.0	0.0	0.0	0.0010656550048487303
13	0.0	0.0	0.0	0.0	0.001229601928671612
14	0.0	0.0	0.0	0.0	0.001229601928671612
15	0.0	0.0	0.0	0.0	0.0013935488524944935
16	0.0	0.0	0.0	8.197346191144079E-5	0.0014755223144059342
17	0.0	0.0	0.0	1.6394692382288157E-4	0.0014755223144059342
18	0.0	0.0	0.0	1.6394692382288157E-4	0.0016394692382288157
19	0.0	0.0	0.0	2.4592038573432236E-4	0.0016394692382288157
20	0.0	0.0	0.0	4.918407714686447E-4	0.001967363085874579
21	0.0	0.0	0.0	5.738142333800855E-4	0.00204933654778602
22	0.0	0.0	0.0	8.197346191144079E-4	0.00204933654778602
23	0.0	0.0	0.0	0.001229601928671612	0.00204933654778602
24	0.0	0.0	0.0	0.001557495776317375	0.00204933654778602
25	0.0	0.0	0.0	0.002459203857343224	0.00204933654778602
26	0.0	0.0	0.0	0.0029510446288118683	0.0021313100096974607
27	0.0	0.0	0.0	0.0037707792479262764	0.002377230395431783
28	0.0	0.0	0.0	0.004426566943217802	0.002459203857343224
29	0.0	0.0	0.0	0.0056561688718894144	0.002459203857343224
30	0.0	0.0	0.0	0.00827931965305552	0.002459203857343224
31	0.0	0.0	0.0	0.012459966210539	0.0025411773192546643
32	0.0	0.0	0.0	0.01762429431095977	0.002705124243077546
33	0.0	0.0	0.0	0.023198489720937743	0.002705124243077546
34	0.0	0.0	0.0	0.029510446288118684	0.0029510446288118683
35	0.0	0.0	0.0	0.03836358017455429	0.0029510446288118683
36	0.0	0.0	0.0	0.04779052829436998	0.0029510446288118683
37	0.0	0.0	0.0	0.06197193720504924	0.0029510446288118683
38	0.0	0.0	0.0	0.08246530268290944	0.0032789384764576315
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTCGT	45	0.0013981451	24.444672	42
TTAACGG	95	2.6862836E-8	23.157162	35
GTATCAA	1790	0.0	21.514753	1
TAACGGC	105	7.745439E-8	20.952578	36
CGTCGTA	95	6.8657937E-7	20.842299	10
ATACGAT	75	5.4740227E-5	20.532684	6
TAGTGCG	55	0.004484456	19.999369	8
GTATAGG	170	5.456968E-12	19.417517	1
GTATATA	185	1.8189894E-12	19.032665	1
CCGTCGT	95	1.5975655E-5	18.525728	9
CGCGGTA	125	4.850008E-7	17.600885	43
ACTAGAC	115	4.2742395E-6	17.216846	3
TAAGACC	260	0.0	16.92254	4
AATACGA	130	7.3193587E-7	16.92254	5
GTATTAG	235	0.0	16.856058	1
CCCGACA	120	6.4085616E-6	16.499477	21
TTATTCC	295	0.0	16.40626	4
GGTATCA	1050	0.0	16.138115	1
TATAGTG	150	1.8382707E-7	16.132822	5
GTATTAC	165	3.0990122E-8	16.004742	1
>>END_MODULE