FastQCFastQC Report
Thu 2 Feb 2017
SRR4062128_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062128_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183155
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA11520.6289754579454561No Hit
GTATCAACGCAGAGTACTTTTTTTT10780.5885725205427097No Hit
TATCAACGCAGAGTACTTTTTTTTT7020.3832819196855123No Hit
GTCCTAAAGTGTGTATTTCTCATTT6490.35434468073489667No Hit
GTCCTACAGTGGACATTTCTAAATT6390.34888482432912016No Hit
CTGTAGGACGTGGAATATGGCAAGA6330.34560891048565423No Hit
CTTTAGGACGTGAAATATGGCGAGG4820.26316507875842865No Hit
GTCCTACAGTGTGCATTTCTCATTT4340.23695776801070131No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4310.23531981108896838No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3760.20529060085719744No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3620.1976468018891103No Hit
CTGTAGGACCTGGAATATGGCGAGA3570.19491687368622207No Hit
GGTATCAACGCAGAGTACTTTTTTT3360.18345117523409132No Hit
CTGAAGGACCTGGAATATGGCGAGA2610.14250225219076737No Hit
ATTTAGAAATGTCCACTGTAGGACG2490.13595042450383557No Hit
GTCCTTCAGTGTGCATTTCTCATTT2430.13267451066036964No Hit
GATATACACTGTTCTACAAATCCCG2270.12393874041112718No Hit
GAATATGGCAAGAAAACTGAAAATC2250.12284676912997189No Hit
TTTCTAAATTTTCCACCTTTTTCAG2050.11192705631841882No Hit
TTGTAGAACAGTGTATATCAATGAG1910.1042832573503317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGA702.4734618E-816.2791561
CTATAGG350.002166194616.2791561
TATAGGA508.676929E-515.1938792
TAGGACC1850.014.8899634
AAATGTC801.2693636E-714.2481557
CATTATA400.005269648514.244262
AGGACCT2650.013.97932155
AATGTCC903.5759513E-813.7204478
GACCTGG2500.012.9183277
GTTACAA604.0565027E-412.66848915
GGACCTG2700.012.6650276
GGCGAGA2600.012.42826319
TTTAGAA1001.4230136E-712.3450272
GAAATGT853.8955222E-612.2925256
TTAGAAA853.8955222E-612.2925253
GTATCAA5350.012.0699021
TGTAGGA4100.012.0439272
TAGAAAT951.024815E-611.9984464
ATGTCCA951.024815E-611.9984469
TGGCGAG4200.011.993118
Produced by FastQC (version 0.11.5)