FastQCFastQC Report
Thu 2 Feb 2017
SRR4062128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183155
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.3778220632797358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.2140263711064399No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA3320.18126723267178074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.18072124703120307No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC2440.1332204963009473No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA2430.13267451066036964No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.1250307116922825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.11902486964592832No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.11465698452130708No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA1890.10319128606917638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTC250.002347672835.1997818
TCTAACA250.002347672835.199782
GAACGCC250.002347672835.1997825
TAGAACC401.8117724E-532.9997984
CGGAATT300.00573263329.33314932
TCGTTCG300.00573263329.33314921
TTCGTTA300.00573263329.33314924
AGTCTCG300.00573263329.33314917
AGTTAGC300.00573263329.3331493
TAGGACC1750.028.9141044
TAGGACT407.009474E-427.499834
TGTAGGA4850.026.7627182
CTGTAGG4900.025.5986651
GTTATTG450.001391528324.4509681
GTAGGAC4800.024.2915153
AGGACCT2650.023.245145
GTATCAA6450.022.8588121
GACGTGG2650.022.4149557
GTTCTGA500.002568180222.005871
GTCCTAT602.8547517E-422.0058691
Produced by FastQC (version 0.11.5)