##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183155
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177554530315852	32.0	32.0	32.0	32.0	32.0
2	31.490366083372006	32.0	32.0	32.0	32.0	32.0
3	31.60810788676258	32.0	32.0	32.0	32.0	32.0
4	31.696639458382244	32.0	32.0	32.0	32.0	32.0
5	31.671584177336136	32.0	32.0	32.0	32.0	32.0
6	35.32017143949114	36.0	36.0	36.0	36.0	36.0
7	35.30464906772952	36.0	36.0	36.0	36.0	36.0
8	35.26836832191314	36.0	36.0	36.0	36.0	36.0
9	35.34129562392509	36.0	36.0	36.0	36.0	36.0
10	35.237531052933306	36.0	36.0	36.0	36.0	36.0
11	35.35364035925855	36.0	36.0	36.0	36.0	36.0
12	35.28331740875215	36.0	36.0	36.0	36.0	36.0
13	35.30152057000901	36.0	36.0	36.0	36.0	36.0
14	35.26722721192433	36.0	36.0	36.0	36.0	36.0
15	35.25513362998553	36.0	36.0	36.0	36.0	36.0
16	35.26627719690972	36.0	36.0	36.0	36.0	36.0
17	35.251491905762876	36.0	36.0	36.0	36.0	36.0
18	35.244841800660645	36.0	36.0	36.0	36.0	36.0
19	35.20593486391308	36.0	36.0	36.0	36.0	36.0
20	35.174742704266876	36.0	36.0	36.0	36.0	36.0
21	35.153951571073684	36.0	36.0	36.0	36.0	36.0
22	35.137653899702435	36.0	36.0	36.0	36.0	36.0
23	35.152471949987714	36.0	36.0	36.0	36.0	36.0
24	35.11643689770959	36.0	36.0	36.0	36.0	36.0
25	35.08777811143567	36.0	36.0	36.0	36.0	36.0
26	35.05808195244465	36.0	36.0	36.0	36.0	36.0
27	35.03454997133576	36.0	36.0	36.0	36.0	36.0
28	34.97603669023505	36.0	36.0	36.0	36.0	36.0
29	34.94758537850455	36.0	36.0	36.0	36.0	36.0
30	34.909759493325325	36.0	36.0	36.0	36.0	36.0
31	34.90571373972865	36.0	36.0	36.0	36.0	36.0
32	34.85267123474652	36.0	36.0	36.0	32.0	36.0
33	34.84072506893069	36.0	36.0	36.0	32.0	36.0
34	34.79456744287625	36.0	36.0	36.0	32.0	36.0
35	34.768283694138844	36.0	36.0	36.0	32.0	36.0
36	34.734656438535666	36.0	36.0	36.0	32.0	36.0
37	34.63690316944664	36.0	36.0	36.0	32.0	36.0
38	34.604739155360214	36.0	36.0	36.0	32.0	36.0
39	34.465665692992275	36.0	36.0	36.0	32.0	36.0
40	34.50876033960307	36.0	36.0	36.0	32.0	36.0
41	34.474057492287955	36.0	36.0	36.0	32.0	36.0
42	34.44341131828233	36.0	36.0	36.0	32.0	36.0
43	34.43557096448363	36.0	36.0	36.0	32.0	36.0
44	34.318773716251265	36.0	36.0	36.0	32.0	36.0
45	34.25972536922279	36.0	36.0	36.0	32.0	36.0
46	34.10865660233136	36.0	36.0	36.0	32.0	36.0
47	34.05115885452213	36.0	36.0	36.0	32.0	36.0
48	33.997018918402446	36.0	36.0	36.0	32.0	36.0
49	33.931937430045586	36.0	36.0	36.0	32.0	36.0
50	33.59173923725806	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	7.0
22	16.0
23	45.0
24	104.0
25	285.0
26	529.0
27	1073.0
28	1886.0
29	2785.0
30	4235.0
31	5836.0
32	8760.0
33	14099.0
34	29448.0
35	114040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.268013085531095	19.84183765421648	12.909127649465054	24.981021610787373
2	13.590027791579534	21.271205411986962	42.83725272865262	22.30151406778088
3	18.963173268543038	26.47047582648576	29.41443040048047	25.151920504490732
4	11.41655974447872	17.349239714995495	38.070486746198576	33.16371379432721
5	11.816221233381562	39.67186263001283	34.710491114083695	13.801425022521908
6	30.53424694930523	38.06393491851164	18.23865032349649	13.163167808686632
7	27.434140482105317	31.831508831317734	21.813218312358384	18.92113237421856
8	24.938440119024868	37.66700335781169	19.856405776528078	17.538150746635363
9	27.17861920231498	14.794572902732659	20.59730829079195	37.42949960416041
10	16.397586743468647	28.18978460866479	32.75859244901859	22.654036198847972
11	34.300455898009886	21.901667986131965	25.791269689607166	18.00660642625099
12	25.379050530971035	24.572083754197266	31.18342387595206	18.865441838879637
13	30.136223417324125	22.04198629576042	26.258633397941633	21.56315688897382
14	21.26122682973438	23.23114302093855	27.56736097840627	27.940269170920807
15	22.364663809341813	32.89563484480358	23.82408342660588	20.915617919248724
16	21.30131638575399	27.303402073676107	29.954737296140387	21.44054424442952
17	20.105375228631488	29.02077475362398	28.998389342360298	21.875460675384236
18	20.50940460265895	26.237885943599682	33.31658977368895	19.936119680052414
19	24.10635800278453	24.622860418770987	28.844967377357978	22.42581420108651
20	24.40774207638339	26.588408724850538	29.263738363681036	19.740110835085037
21	23.462640932543476	25.121891294258962	27.78302530643444	23.632442466763123
22	23.583303759111136	28.151019628183775	28.09314515028255	20.172531462422537
23	21.209904179520077	28.12590428871721	29.56894433676394	21.09524719499877
24	23.34836638420547	25.93583471651961	30.38569057394951	20.330108325325412
25	22.001823611952847	27.338891527848304	30.295436056193452	20.363848804005393
26	19.941688415213427	28.36847680094347	30.89094423272219	20.798890551120916
27	20.702043668885235	28.214970161233083	29.724653428045713	21.358332741835973
28	20.24427664302523	28.186816486762435	28.85729417481559	22.711612695396745
29	21.56842404123351	26.83290381759413	28.16513060190443	23.433541539267928
30	20.78644630445584	26.909196136563416	30.054653759425182	22.24970379955556
31	23.512999989080228	25.54899155901591	29.00564552234731	21.932362929556547
32	21.028205772191708	28.420345719995193	29.25188639068762	21.29956211712548
33	20.23761424811907	28.377758607510618	30.746257247998955	20.63836989637136
34	21.115448663700146	27.919521716578856	30.793590128579616	20.17143949114138
35	21.122000491387077	28.635854877016737	29.95550217029292	20.286642461303266
36	20.094455515819934	27.823974229477766	30.704594469165457	21.37697578553684
37	21.024929840462125	27.77498716926739	28.237439531760156	22.962643458510325
38	21.427206464469982	29.155087221206085	28.26185471322104	21.15585160110289
39	23.035259945182744	26.828788888039572	28.521899603612262	21.61405156316542
40	23.822575537525797	26.435677080489643	29.505771099730282	20.235976282254278
41	23.112664136933198	26.232426087193904	30.059239442002678	20.59567033387022
42	21.955161455887655	29.085538313695086	29.12212114924705	19.837179081170213
43	20.34332326877822	28.95042888108719	29.837128926404986	20.8691189237296
44	20.144034157607656	28.909479063723374	28.952612871346595	21.993873907322374
45	20.41178461840855	29.144326632232985	28.86587243521845	21.578016314140015
46	21.351431036177207	26.852812387389847	28.508249888072335	23.287506688360615
47	23.1594522582336	27.13593081156635	28.344762819952823	21.359854110247227
48	21.115594406739866	28.387269381760206	27.65125662711781	22.845879584382114
49	22.45300223317881	27.56474307523465	27.67776703959115	22.30448765199539
50	21.176714677266126	29.830088340958977	27.119800823350843	21.873396158424057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	443.0
1	248.5
2	54.0
3	170.0
4	286.0
5	235.5
6	185.0
7	137.5
8	90.0
9	144.5
10	199.0
11	303.0
12	407.0
13	696.0
14	985.0
15	1251.0
16	1517.0
17	1464.0
18	1411.0
19	1277.5
20	1144.0
21	1207.0
22	1270.0
23	1364.5
24	1459.0
25	1764.0
26	2069.0
27	2916.0
28	3763.0
29	4691.0
30	5619.0
31	6671.5
32	7724.0
33	9425.0
34	11126.0
35	12370.5
36	13615.0
37	13680.0
38	13745.0
39	12923.0
40	12101.0
41	11294.0
42	10487.0
43	9981.0
44	9475.0
45	9472.5
46	9470.0
47	9748.5
48	10027.0
49	10041.5
50	10056.0
51	9686.0
52	9316.0
53	9059.5
54	8803.0
55	8538.0
56	8273.0
57	7802.5
58	7332.0
59	6660.5
60	5989.0
61	5179.0
62	4369.0
63	3690.5
64	3012.0
65	2584.5
66	2157.0
67	1897.0
68	1637.0
69	1495.0
70	1353.0
71	1089.0
72	825.0
73	679.5
74	534.0
75	424.0
76	314.0
77	255.0
78	196.0
79	168.5
80	141.0
81	110.5
82	80.0
83	63.0
84	46.0
85	35.5
86	25.0
87	18.0
88	11.0
89	9.5
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02839125331003795
2	0.003275913843465917
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010919712811553056
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0016379569217329585
25	0.0010919712811553056
26	5.459856405776528E-4
27	0.002183942562310611
28	0.0010919712811553056
29	0.0016379569217329585
30	0.0010919712811553056
31	5.459856405776528E-4
32	5.459856405776528E-4
33	5.459856405776528E-4
34	0.0
35	0.0
36	0.0
37	5.459856405776528E-4
38	0.0
39	5.459856405776528E-4
40	5.459856405776528E-4
41	0.0
42	0.0049138707651988755
43	0.002183942562310611
44	0.002183942562310611
45	5.459856405776528E-4
46	5.459856405776528E-4
47	0.0016379569217329585
48	0.003275913843465917
49	0.004367885124621222
50	5.459856405776528E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	183155.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.681164923840036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75146826477763	48.23941743792339
2	15.985195207607926	20.35911704710916
3	4.197936527709919	8.019884650827231
4	1.5527530194659394	3.9552448447440436
5	0.7253605661436	2.309590292092029
6	0.39018913176160613	1.4908619070720475
7	0.2297225188797701	1.0240298328051818
8	0.18020704130253137	0.9180635454099003
9	0.16040272000439404	0.9193168860149117
>10	0.7663666079181178	8.502082869253817
>50	0.04356605499765007	1.800166480867792
>100	0.015842201817327296	2.0258966385387795
>500	9.90137613582956E-4	0.4363275673416855
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	692	0.3778220632797358	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.2140263711064399	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	332	0.18126723267178074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	331	0.18072124703120307	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	244	0.1332204963009473	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	243	0.13267451066036964	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	229	0.1250307116922825	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	218	0.11902486964592832	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	210	0.11465698452130708	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	189	0.10319128606917638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.459856405776528E-4
7	0.0	0.0	0.0	0.0	5.459856405776528E-4
8	0.0	0.0	0.0	0.0	5.459856405776528E-4
9	0.0	0.0	0.0	0.0	5.459856405776528E-4
10	0.0	0.0	0.0	0.0	5.459856405776528E-4
11	0.0	0.0	0.0	0.0	5.459856405776528E-4
12	0.0	0.0	0.0	0.0	5.459856405776528E-4
13	0.0	0.0	0.0	0.0	5.459856405776528E-4
14	0.0	0.0	0.0	0.0	5.459856405776528E-4
15	0.0	0.0	0.0	0.0	0.0010919712811553056
16	0.0	0.0	0.0	0.0	0.0010919712811553056
17	0.0	0.0	0.0	0.0	0.0010919712811553056
18	0.0	0.0	0.0	0.0	0.0010919712811553056
19	0.0	0.0	0.0	0.0	0.0010919712811553056
20	0.0	0.0	0.0	0.0	0.0010919712811553056
21	0.0	0.0	0.0	0.0	0.0010919712811553056
22	0.0	0.0	0.0	5.459856405776528E-4	0.0010919712811553056
23	0.0	0.0	0.0	0.002729928202888264	0.0010919712811553056
24	0.0	0.0	0.0	0.003275913843465917	0.0010919712811553056
25	0.0	0.0	0.0	0.0038218994840435697	0.0010919712811553056
26	0.0	0.0	0.0	0.0049138707651988755	0.0010919712811553056
27	0.0	0.0	0.0	0.006005842046354181	0.0010919712811553056
28	0.0	0.0	0.0	0.007643798968087139	0.0010919712811553056
29	0.0	0.0	0.0	0.010373727170975403	0.0010919712811553056
30	0.0	0.0	0.0	0.01747154049848489	0.0010919712811553056
31	0.0	0.0	0.0	0.025115339466572028	0.0010919712811553056
32	0.0	0.0	0.0	0.03603505227812508	0.0010919712811553056
33	0.0	0.0	0.0	0.04477082252736753	0.0010919712811553056
34	0.0	0.0	0.0	0.05132265021429937	0.0010919712811553056
35	0.0	0.0	0.0	0.06551827686931834	0.0010919712811553056
36	0.0	0.0	0.0	0.08353580300838089	0.0010919712811553056
37	0.0	0.0	0.0	0.11138107067784117	0.0010919712811553056
38	0.0	0.0	0.0	0.14414020911250033	0.0010919712811553056
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTC	25	0.0023476728	35.19978	18
TCTAACA	25	0.0023476728	35.19978	2
GAACGCC	25	0.0023476728	35.19978	25
TAGAACC	40	1.8117724E-5	32.999798	4
CGGAATT	30	0.005732633	29.333149	32
TCGTTCG	30	0.005732633	29.333149	21
TTCGTTA	30	0.005732633	29.333149	24
AGTCTCG	30	0.005732633	29.333149	17
AGTTAGC	30	0.005732633	29.333149	3
TAGGACC	175	0.0	28.914104	4
TAGGACT	40	7.009474E-4	27.49983	4
TGTAGGA	485	0.0	26.762718	2
CTGTAGG	490	0.0	25.598665	1
GTTATTG	45	0.0013915283	24.450968	1
GTAGGAC	480	0.0	24.291515	3
AGGACCT	265	0.0	23.24514	5
GTATCAA	645	0.0	22.858812	1
GACGTGG	265	0.0	22.414955	7
GTTCTGA	50	0.0025681802	22.00587	1
GTCCTAT	60	2.8547517E-4	22.005869	1
>>END_MODULE