FastQCFastQC Report
Thu 2 Feb 2017
SRR4062127_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062127_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768607
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25240.14271118456502774No Hit
GCGCAAGACGGACCAGAGCGAAAGC24670.13948830916082544No Hit
GATTAAGAGGGACGGCCGGGGGCAT21330.12060339012567517No Hit
GGGTAGGCACACGCTGAGCCAGTCA20950.11845480652287366No Hit
GAATAGGACCGCGGTTCTATTTTGT20580.11636276459383006No Hit
ATCAGATACCGTCGTAGTTCCGACC20570.11630622292007212No Hit
TCGTAGTTCCGACCATAAACGATGC20160.11398801429599678No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19210.10861655528899299No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC18540.10482826314721133No Hit
GAACTACGACGGTATCTGATCGTCT17970.10160538774300905No Hit
GTATCTGATCGTCTTCGAACCTCCG17790.10058763761536622No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17730.1002483875728186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA1550.012.8730599
GTATCAA17450.012.8407681
GCGTAAC1950.012.66944311
CGCGTAA1950.012.66908510
TCCAACG1352.7284841E-1112.6647818
CGGTCCA3100.012.56691410
CTCGTAG1600.012.4711310
CCGACCA3800.012.251999
CGACCAT3650.012.23521110
GGTATCA6550.012.1762181
TAGATCG550.003067600312.0915035
CGAACGA1650.012.09116116
TCGCGTA2050.012.0507399
CGGCGTC1353.7107384E-1011.96389114
CGGACCA4450.011.9579
CGTCGTA3600.011.87726610
CCTATTC3800.011.7465853
ACGAACG1701.8189894E-1211.73620415
CGGTTCT3250.011.69487112
TCGGCGT1302.6102498E-911.69387613
Produced by FastQC (version 0.11.5)