##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062127_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768607
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301868080359288	32.0	32.0	32.0	32.0	32.0
2	30.848024462189734	32.0	32.0	32.0	32.0	32.0
3	30.875234577269005	32.0	32.0	32.0	32.0	32.0
4	30.885512157307982	32.0	32.0	32.0	32.0	32.0
5	30.802826744437855	32.0	32.0	32.0	32.0	32.0
6	34.46111940074872	36.0	36.0	36.0	32.0	36.0
7	34.395445115845405	36.0	36.0	36.0	32.0	36.0
8	34.35286527758852	36.0	36.0	36.0	32.0	36.0
9	34.48825940415254	36.0	36.0	36.0	32.0	36.0
10	34.18183067238793	36.0	36.0	36.0	32.0	36.0
11	34.472708182202155	36.0	36.0	36.0	32.0	36.0
12	34.28350786805661	36.0	36.0	36.0	32.0	36.0
13	34.385521486684155	36.0	36.0	36.0	32.0	36.0
14	34.291820059515764	36.0	36.0	36.0	32.0	36.0
15	34.235212797416274	36.0	36.0	36.0	32.0	36.0
16	34.23574824706676	36.0	36.0	36.0	32.0	36.0
17	34.17372429262125	36.0	36.0	36.0	32.0	36.0
18	34.17971884087307	36.0	36.0	36.0	32.0	36.0
19	34.17533742657357	36.0	36.0	36.0	32.0	36.0
20	34.165374218240686	36.0	36.0	36.0	32.0	36.0
21	34.13215881199159	36.0	36.0	36.0	32.0	36.0
22	34.1051545086048	36.0	36.0	36.0	32.0	36.0
23	34.07276291454235	36.0	36.0	36.0	32.0	36.0
24	34.03644337040394	36.0	36.0	36.0	32.0	36.0
25	33.689297283116034	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	22.0
5	145.0
6	344.0
7	83.0
8	295.0
9	248.0
10	134.0
11	31.0
12	64.0
13	81.0
14	219.0
15	365.0
16	558.0
17	726.0
18	999.0
19	1386.0
20	2090.0
21	3047.0
22	4539.0
23	6669.0
24	9784.0
25	13393.0
26	18740.0
27	24796.0
28	33778.0
29	44797.0
30	59662.0
31	81742.0
32	117967.0
33	168653.0
34	373945.0
35	799305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03205101021324	17.361486881900635	11.621593961733295	25.984868146152834
2	16.65190924809539	19.415956783945738	37.64114255214005	26.29099141581882
3	19.448749489001735	22.951186731629647	27.891656493819678	29.708407285548944
4	12.73308169699026	15.547506962410054	35.24271597608041	36.47669536451927
5	14.91238290666356	36.47265833123119	33.240820029479075	15.374138732626182
6	35.074387048008724	35.034443896279065	16.386706285445303	13.504462770266906
7	30.809526720460784	29.89495883935121	20.10226768458898	19.193246755599024
8	27.98413750514382	33.5291617115238	18.925975967392	19.560724815940382
9	26.838639044813426	14.54483494138772	18.555932441336772	40.060593572462075
10	16.443418301515795	26.727921964321403	30.70494924612276	26.123710488040043
11	37.49504576018988	21.10268355600046	22.000844768662482	19.40142591514718
12	24.560611868107976	23.560817386765166	28.586512880584603	23.292057864542258
13	29.478207850687816	19.423140829928563	24.963414143964567	26.135237175419064
14	23.34943602829878	20.043722949114052	24.659313090053196	31.94752793253397
15	25.050558235387367	27.754149964897977	21.702165002151414	25.493126797563242
16	25.787462306024977	25.700795175171198	23.819195722232696	24.69254679657113
17	23.908487541099426	26.222113529523654	25.06147930762751	24.80791962174941
18	24.755302625501507	25.145358051749277	26.193713377469045	23.905625945280164
19	25.67078322986745	24.95337951143426	24.79802504574326	24.577812212955035
20	25.627135679529506	24.784408846169953	24.522100144764973	25.06635532953556
21	26.625957481727557	24.40570343604008	24.214419314037027	24.75391976819534
22	25.87423946571582	24.704530661912443	24.77969267340182	24.641537198969917
23	24.35783754236238	24.72797255308277	25.412391289463006	25.501798615091843
24	24.821167841903318	25.147649715339952	25.191508868037715	24.83967357471902
25	24.739358002669942	24.86374406577182	25.154619572752544	25.24227835880569
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	699.0
1	699.0
2	600.5
3	502.0
4	502.0
5	502.0
6	1113.0
7	1724.0
8	1724.0
9	1724.0
10	2136.5
11	2549.0
12	2549.0
13	2549.0
14	3428.5
15	4308.0
16	4308.0
17	4308.0
18	7419.0
19	10530.0
20	10530.0
21	10530.0
22	17416.5
23	24303.0
24	24303.0
25	24303.0
26	36861.5
27	49420.0
28	49420.0
29	49420.0
30	63681.0
31	77942.0
32	77942.0
33	77942.0
34	94766.5
35	111591.0
36	111591.0
37	111591.0
38	128515.5
39	145440.0
40	145440.0
41	145440.0
42	165420.0
43	185400.0
44	185400.0
45	185400.0
46	204043.0
47	222686.0
48	222686.0
49	222686.0
50	228579.5
51	234473.0
52	234473.0
53	234473.0
54	219238.0
55	204003.0
56	204003.0
57	204003.0
58	190542.5
59	177082.0
60	177082.0
61	177082.0
62	156240.5
63	135399.0
64	135399.0
65	135399.0
66	111513.5
67	87628.0
68	87628.0
69	87628.0
70	66263.5
71	44899.0
72	44899.0
73	44899.0
74	34269.5
75	23640.0
76	23640.0
77	23640.0
78	19142.5
79	14645.0
80	14645.0
81	14645.0
82	9980.5
83	5316.0
84	5316.0
85	5316.0
86	3999.0
87	2682.0
88	2682.0
89	2682.0
90	1788.5
91	895.0
92	895.0
93	895.0
94	543.0
95	191.0
96	191.0
97	191.0
98	425.5
99	660.0
100	660.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011308334751586983
2	2.827083687896746E-4
3	5.654167375793492E-4
4	0.010290584623944155
5	0.03239837906329671
6	0.06191313276493873
7	0.10578947160109622
8	0.10878618031026678
9	0.11777630643777845
10	0.13219443324605185
11	0.138131308990635
12	0.13253368328859944
13	0.1251832657000679
14	0.11472305605484995
15	0.13157247483471454
16	0.11749359806898876
17	0.12207347364338147
18	0.10940813872160407
19	0.09499001191333066
20	0.09074938638148555
21	0.09057976136021173
22	0.09934372079269166
23	0.07994992669371997
24	0.08973163625384271
25	0.08639567750212455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1768607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.51743178605277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.34972501295236	32.72488534457582
2	16.176156010341682	15.049464675432333
3	5.96413928645831	8.323093272610254
4	2.6857656572057222	4.997396830095614
5	1.4163999931506517	3.294364503157552
6	0.8163980951356009	2.2786045620440234
7	0.5312811905629113	1.729968557885619
8	0.3552504087001606	1.322026932294167
9	0.2649360604002216	1.1091730605599577
>10	1.2500308946601497	10.283595406580194
>50	0.08820498242835893	2.9017646068692233
>100	0.08293148128545835	8.130499757167984
>500	0.013414947655933668	4.200225150224208
>1k	0.0053659790623734665	3.654937340502911
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2524	0.14271118456502774	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2467	0.13948830916082544	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2133	0.12060339012567517	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2095	0.11845480652287366	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2058	0.11636276459383006	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2057	0.11630622292007212	No Hit
TCGTAGTTCCGACCATAAACGATGC	2016	0.11398801429599678	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1921	0.10861655528899299	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1854	0.10482826314721133	No Hit
GAACTACGACGGTATCTGATCGTCT	1797	0.10160538774300905	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1779	0.10058763761536622	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1773	0.1002483875728186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.392500425476095E-4
2	0.0	0.0	0.0	0.0	3.392500425476095E-4
3	0.0	0.0	0.0	0.0	3.392500425476095E-4
4	0.0	0.0	0.0	0.0	3.392500425476095E-4
5	0.0	0.0	0.0	0.0	3.9579171630554444E-4
6	0.0	0.0	0.0	0.0	4.523333900634793E-4
7	0.0	0.0	0.0	0.0	4.523333900634793E-4
8	0.0	0.0	0.0	0.0	4.523333900634793E-4
9	0.0	0.0	0.0	0.0	4.523333900634793E-4
10	0.0	0.0	0.0	0.0	5.654167375793492E-4
11	0.0	0.0	0.0	0.0	5.654167375793492E-4
12	0.0	0.0	0.0	0.0	9.612084538848936E-4
13	0.0	0.0	0.0	0.0	0.0011873751489166332
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	155	0.0	12.873059	9
GTATCAA	1745	0.0	12.840768	1
GCGTAAC	195	0.0	12.669443	11
CGCGTAA	195	0.0	12.669085	10
TCCAACG	135	2.7284841E-11	12.66478	18
CGGTCCA	310	0.0	12.566914	10
CTCGTAG	160	0.0	12.47113	10
CCGACCA	380	0.0	12.25199	9
CGACCAT	365	0.0	12.235211	10
GGTATCA	655	0.0	12.176218	1
TAGATCG	55	0.0030676003	12.091503	5
CGAACGA	165	0.0	12.091161	16
TCGCGTA	205	0.0	12.050739	9
CGGCGTC	135	3.7107384E-10	11.963891	14
CGGACCA	445	0.0	11.957	9
CGTCGTA	360	0.0	11.877266	10
CCTATTC	380	0.0	11.746585	3
ACGAACG	170	1.8189894E-12	11.736204	15
CGGTTCT	325	0.0	11.694871	12
TCGGCGT	130	2.6102498E-9	11.693876	13
>>END_MODULE