FastQCFastQC Report
Thu 2 Feb 2017
SRR4062127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768607
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25550.1444639764515237No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22970.1298762246219765No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT21400.12099918184198073No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21220.11998143171433788No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG20780.11749359806898876No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20620.1165889312888618No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20000.11308334751586983No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17870.10103997100542969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.025.308911
GTATCAA22600.024.7343711
CGCAATA4950.020.00082236
GTCCTAC7550.019.2385641
TAGGACG5000.018.9186364
CGTCGTA6400.018.90542610
ATACCGT6200.018.8050986
CAATACG5300.018.68265338
ATACGAA5200.018.61878240
GTCCTAG3450.018.4992681
ACCGTCG6450.018.417288
TACGAAT5250.018.02233741
CTAGCGG5200.017.76795229
GTAGGAC8550.017.7531073
TACCGTC6600.017.6653947
CCGTCGT6750.017.5987349
GGCGTTA6600.017.33649642
CCTATTC6000.017.2320923
ACGGACC9100.017.1635998
CGGACCA9100.017.1635999
Produced by FastQC (version 0.11.5)