##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768607
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283036310497472	32.0	32.0	32.0	32.0	32.0
2	31.44812668953589	32.0	32.0	32.0	32.0	32.0
3	31.576019432242436	32.0	32.0	32.0	32.0	32.0
4	31.656155381042822	32.0	32.0	32.0	32.0	32.0
5	31.62126577583375	32.0	32.0	32.0	32.0	32.0
6	35.27471281070356	36.0	36.0	36.0	36.0	36.0
7	35.27394327852372	36.0	36.0	36.0	36.0	36.0
8	35.230566768083584	36.0	36.0	36.0	36.0	36.0
9	35.318139643233344	36.0	36.0	36.0	36.0	36.0
10	35.1869171613592	36.0	36.0	36.0	36.0	36.0
11	35.316292992168414	36.0	36.0	36.0	36.0	36.0
12	35.22869636951567	36.0	36.0	36.0	36.0	36.0
13	35.27260097918871	36.0	36.0	36.0	36.0	36.0
14	35.23436071439274	36.0	36.0	36.0	36.0	36.0
15	35.209554185864924	36.0	36.0	36.0	36.0	36.0
16	35.21895650079413	36.0	36.0	36.0	36.0	36.0
17	35.19780821855845	36.0	36.0	36.0	36.0	36.0
18	35.202195287025326	36.0	36.0	36.0	36.0	36.0
19	35.18716820639068	36.0	36.0	36.0	36.0	36.0
20	35.18243171037998	36.0	36.0	36.0	36.0	36.0
21	35.17101877353194	36.0	36.0	36.0	36.0	36.0
22	35.153898520134774	36.0	36.0	36.0	36.0	36.0
23	35.118370559428975	36.0	36.0	36.0	36.0	36.0
24	35.11079114806172	36.0	36.0	36.0	36.0	36.0
25	35.08332263753338	36.0	36.0	36.0	36.0	36.0
26	35.04478609436692	36.0	36.0	36.0	36.0	36.0
27	35.03255386866613	36.0	36.0	36.0	36.0	36.0
28	35.007994992669374	36.0	36.0	36.0	36.0	36.0
29	34.98544278067428	36.0	36.0	36.0	36.0	36.0
30	34.961183010131705	36.0	36.0	36.0	36.0	36.0
31	34.96230253527211	36.0	36.0	36.0	36.0	36.0
32	34.933610462923646	36.0	36.0	36.0	36.0	36.0
33	34.923226019121266	36.0	36.0	36.0	32.0	36.0
34	34.908724776052566	36.0	36.0	36.0	32.0	36.0
35	34.893367492043176	36.0	36.0	36.0	32.0	36.0
36	34.868161779298624	36.0	36.0	36.0	32.0	36.0
37	34.85101268964784	36.0	36.0	36.0	32.0	36.0
38	34.82317213490617	36.0	36.0	36.0	32.0	36.0
39	34.79318695447886	36.0	36.0	36.0	32.0	36.0
40	34.7938569733129	36.0	36.0	36.0	32.0	36.0
41	34.767519296259714	36.0	36.0	36.0	32.0	36.0
42	34.736956825343334	36.0	36.0	36.0	32.0	36.0
43	34.737048422854826	36.0	36.0	36.0	32.0	36.0
44	34.68406604745995	36.0	36.0	36.0	32.0	36.0
45	34.67439007082975	36.0	36.0	36.0	32.0	36.0
46	34.641884262586316	36.0	36.0	36.0	32.0	36.0
47	34.62503823630688	36.0	36.0	36.0	32.0	36.0
48	34.59139537500417	36.0	36.0	36.0	32.0	36.0
49	34.5733467073239	36.0	36.0	36.0	32.0	36.0
50	34.1645679339729	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	2.0
20	18.0
21	39.0
22	122.0
23	325.0
24	770.0
25	1859.0
26	4214.0
27	7914.0
28	13797.0
29	21923.0
30	32973.0
31	48998.0
32	74689.0
33	128403.0
34	296319.0
35	1136239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81370647089764	17.88882793102083	12.02548541630139	26.271980181780137
2	16.048559691723987	19.87752644122711	37.47673935353638	26.597174513512524
3	18.835304102286614	23.355209405863057	28.219398518262196	29.590087973588137
4	12.47258435593662	15.90387236961066	35.48170961666442	36.141833657788304
5	14.594084497008097	36.805972157749004	33.09700798424975	15.502935360993142
6	35.0718221423099	35.26802197211927	16.29521572086475	13.364940164706082
7	30.706143309395472	29.89799316637331	20.28302500216272	19.112838522068497
8	27.959122631539962	33.64919397016975	18.98081371384372	19.41086968444657
9	27.04970635081734	14.267952122772328	18.502075362135283	40.18026616427505
10	16.415263111470967	26.73034777711687	30.74967601565981	26.10471309575236
11	37.746203650669706	21.01823638603715	21.956036586986254	19.27952337630689
12	24.57951370768068	23.46456844284796	28.67957663856357	23.276341210907795
13	29.527814828280107	19.346129467993737	25.10783910727482	26.01821659645133
14	23.48814632080502	19.81429452670944	24.69423676373553	32.003322388750014
15	25.098735897799795	27.67517034592761	21.717034932011465	25.50905882426113
16	25.79351078482593	25.639659933642278	23.883521165941797	24.683308115589995
17	24.08771422933416	26.18693695094501	24.97287413201463	24.7524746877062
18	24.889870954937983	24.99074130092214	26.301546923652342	23.817840820487536
19	25.809196621519998	24.77906328486955	24.93709735237809	24.474642741232362
20	25.84210579881737	24.578621985048077	24.617126973734056	24.962145242400492
21	26.77779744171543	24.224658163175878	24.324171508989846	24.673372886118848
22	25.97722388297683	24.53071824322758	24.866688868697228	24.625369005098364
23	24.48069270374521	24.559681466646612	25.50211861771361	25.457507211894566
24	24.99024648958438	24.925166544724227	25.274483572826778	24.810103392864615
25	24.947769510738134	24.520313490364995	25.394053951056282	25.137863047840586
26	24.381644022591868	25.702801253646246	25.64846452082748	24.267090202934412
27	25.087739038345084	24.834261281682597	25.178997815782793	24.899001864189525
28	24.653168078345338	24.846034671883658	25.53856766445397	24.962229585317033
29	24.456844703808265	24.80876127515477	25.404034035941404	25.330359985095562
30	24.403054191314418	25.17434832120123	25.781782734908	24.64081475257635
31	25.320691326082027	24.813963126787783	24.783882854301226	25.081462692828964
32	25.022517874357895	24.918876283151313	24.740259923837847	25.31834591865294
33	24.459954506414956	24.653158061088963	25.488507583112302	25.39837984938378
34	25.252984444644227	24.966314916936515	25.49051870221821	24.290181936201048
35	25.5482566495873	24.571385599732217	25.697639836119517	24.182717914560957
36	24.188598251956623	25.416457933536357	25.161398298999327	25.233545515507693
37	25.371316519768488	25.132479763334487	24.936898243328514	24.559305473568507
38	24.768306356358423	24.79572906813102	25.20045436889032	25.235510206620237
39	25.57011195295714	24.52725319461721	24.732387198914395	25.17024765351125
40	25.78985935816117	24.87563651825223	25.169031429272547	24.16547269431405
41	24.504208446747853	25.164170699078248	25.903744573348426	24.427876280825473
42	25.832527579402104	25.519329612729212	24.943031783458576	23.7051110244101
43	25.12856671927135	24.445120458322332	25.3596616864674	25.066651135938915
44	24.483525282042468	25.166183402241867	25.06202898681842	25.288262328897254
45	24.79429638961327	25.394161590985682	25.012382927618116	24.799159091782936
46	24.558613073930225	25.006375196274057	25.038943826463484	25.396067903332238
47	24.95117568498224	24.928558746931166	25.381745643129594	24.738519924957
48	25.671170539514815	25.304542925511946	24.337367518738493	24.68691901623475
49	24.850894969443928	25.58143611270689	24.59198259366985	24.97568632417933
50	25.0627619321176	25.804370492166633	24.484334395574322	24.648533180141445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	335.0
1	196.0
2	57.0
3	157.5
4	258.0
5	248.0
6	238.0
7	205.5
8	173.0
9	235.5
10	298.0
11	481.0
12	664.0
13	1213.0
14	1762.0
15	2300.5
16	2839.0
17	2865.5
18	2892.0
19	2962.5
20	3033.0
21	3701.5
22	4370.0
23	5319.5
24	6269.0
25	8328.0
26	10387.0
27	13959.0
28	17531.0
29	21107.0
30	24683.0
31	27678.0
32	30673.0
33	35578.5
34	40484.0
35	46750.0
36	53016.0
37	61486.5
38	69957.0
39	74983.5
40	80010.0
41	85298.5
42	90587.0
43	92723.5
44	94860.0
45	103193.5
46	111527.0
47	118802.5
48	126078.0
49	131262.5
50	136447.0
51	131453.5
52	126460.0
53	125737.5
54	125015.0
55	126401.5
56	127788.0
57	125131.5
58	122475.0
59	111872.5
60	101270.0
61	88830.5
62	76391.0
63	65896.0
64	55401.0
65	46566.5
66	37732.0
67	32969.0
68	28206.0
69	25454.5
70	22703.0
71	17566.0
72	12429.0
73	10930.0
74	9431.0
75	6895.0
76	4359.0
77	3977.0
78	3595.0
79	3032.5
80	2470.0
81	1973.5
82	1477.0
83	1265.5
84	1054.0
85	803.0
86	552.0
87	377.5
88	203.0
89	143.5
90	84.0
91	51.0
92	18.0
93	23.5
94	29.0
95	20.5
96	12.0
97	14.0
98	16.0
99	12.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03036287880801105
2	0.003505583772991965
3	4.5233339006347937E-4
4	0.0
5	0.0
6	1.1308334751586984E-4
7	0.0
8	0.0
9	0.0
10	1.6962502127380476E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.219584113372842E-4
17	0.0
18	0.0
19	5.654167375793492E-5
20	2.827083687896746E-4
21	0.0
22	0.0
23	5.654167375793492E-5
24	7.350417588531539E-4
25	2.2616669503173968E-4
26	3.392500425476095E-4
27	5.654167375793492E-4
28	0.0015266251914642429
29	3.392500425476095E-4
30	0.0011873751489166332
31	3.392500425476095E-4
32	6.78500085095219E-4
33	3.392500425476095E-4
34	0.0011308334751586983
35	5.654167375793492E-5
36	5.654167375793492E-5
37	0.001978958581527722
38	0.0
39	3.9579171630554444E-4
40	5.654167375793492E-5
41	0.0011873751489166332
42	0.004353708879360989
43	0.003957917163055444
44	0.004466792226876858
45	0.0024312919715912015
46	0.0019224169077697872
47	0.0011873751489166332
48	0.0031097920566864204
49	0.0031097920566864204
50	0.0010742918014007635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1768607.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.1094899064655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.06854480617766	33.70961949113529
2	16.39000713863435	15.770297660060534
3	6.073685924892002	8.766057950959
4	2.706328657196463	5.208003648678862
5	1.4328763114185479	3.4467474220702075
6	0.84913151425863	2.4510770408692393
7	0.5250385164411708	1.768153464506249
8	0.3669868160902899	1.4124438819601368
9	0.25052984181620885	1.0847576606512768
>10	1.1711424990561183	9.746747895185766
>50	0.07834234957772898	2.6739105129445724
>100	0.0721527661300077	7.334423696213283
>500	0.010745659738668017	3.501295015472798
>1k	0.00448719857219104	3.1264646592927368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2555	0.1444639764515237	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2297	0.1298762246219765	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2140	0.12099918184198073	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2122	0.11998143171433788	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2078	0.11749359806898876	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2062	0.1165889312888618	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2000	0.11308334751586983	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1787	0.10103997100542969	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6962502127380476E-4
2	0.0	0.0	0.0	0.0	1.6962502127380476E-4
3	0.0	0.0	0.0	0.0	1.6962502127380476E-4
4	0.0	0.0	0.0	0.0	1.6962502127380476E-4
5	0.0	0.0	0.0	0.0	1.6962502127380476E-4
6	0.0	0.0	0.0	0.0	2.827083687896746E-4
7	0.0	0.0	0.0	0.0	2.827083687896746E-4
8	0.0	0.0	0.0	0.0	2.827083687896746E-4
9	0.0	0.0	0.0	0.0	2.827083687896746E-4
10	0.0	0.0	0.0	0.0	2.827083687896746E-4
11	0.0	0.0	0.0	0.0	3.392500425476095E-4
12	0.0	0.0	0.0	0.0	0.0014700835177063078
13	0.0	0.0	0.0	0.0	0.0016962502127380475
14	0.0	0.0	0.0	0.0	0.0016962502127380475
15	0.0	0.0	0.0	1.6962502127380476E-4	0.0018658752340118523
16	0.0	0.0	0.0	2.827083687896746E-4	0.001978958581527722
17	0.0	0.0	0.0	2.827083687896746E-4	0.002035500255285657
18	0.0	0.0	0.0	3.9579171630554444E-4	0.002035500255285657
19	0.0	0.0	0.0	4.523333900634793E-4	0.002035500255285657
20	0.0	0.0	0.0	5.654167375793492E-4	0.0022616669503173967
21	0.0	0.0	0.0	7.350417588531539E-4	0.0025443753191070713
22	0.0	0.0	0.0	9.046667801269586E-4	0.0026009169928650063
23	0.0	0.0	0.0	0.0015266251914642427	0.0026009169928650063
24	0.0	0.0	0.0	0.0017527918864959824	0.0026009169928650063
25	0.0	0.0	0.0	0.0019224169077697872	0.0026009169928650063
26	0.0	0.0	0.0	0.0022616669503173967	0.002657458666622941
27	0.0	0.0	0.0	0.002883625361654681	0.0027705420141388108
28	0.0	0.0	0.0	0.003901375489297509	0.002827083687896746
29	0.0	0.0	0.0	0.005201833985730013	0.002827083687896746
30	0.0	0.0	0.0	0.006728459177194255	0.002827083687896746
31	0.0	0.0	0.0	0.010403667971460025	0.0029401670354126156
32	0.0	0.0	0.0	0.014700835177063078	0.0029967087091705507
33	0.0	0.0	0.0	0.018658752340118524	0.0029967087091705507
34	0.0	0.0	0.0	0.023069002893237447	0.00322287540420229
35	0.0	0.0	0.0	0.028949336964062676	0.00322287540420229
36	0.0	0.0	0.0	0.035225462751193454	0.00322287540420229
37	0.0	0.0	0.0	0.04800388102048674	0.00322287540420229
38	0.0	0.0	0.0	0.06151734104863319	0.00344904209923403
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	25.30891	1
GTATCAA	2260	0.0	24.734371	1
CGCAATA	495	0.0	20.000822	36
GTCCTAC	755	0.0	19.238564	1
TAGGACG	500	0.0	18.918636	4
CGTCGTA	640	0.0	18.905426	10
ATACCGT	620	0.0	18.805098	6
CAATACG	530	0.0	18.682653	38
ATACGAA	520	0.0	18.618782	40
GTCCTAG	345	0.0	18.499268	1
ACCGTCG	645	0.0	18.41728	8
TACGAAT	525	0.0	18.022337	41
CTAGCGG	520	0.0	17.767952	29
GTAGGAC	855	0.0	17.753107	3
TACCGTC	660	0.0	17.665394	7
CCGTCGT	675	0.0	17.598734	9
GGCGTTA	660	0.0	17.336496	42
CCTATTC	600	0.0	17.232092	3
ACGGACC	910	0.0	17.163599	8
CGGACCA	910	0.0	17.163599	9
>>END_MODULE