FastQCFastQC Report
Thu 2 Feb 2017
SRR4062126_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062126_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002248
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18420.1837868471675673No Hit
TATCAACGCAGAGTACTTTTTTTTT11580.11554026548319378No Hit
GCGCAAGACGGACCAGAGCGAAAGC11050.110252153159697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAC456.745893E-414.7816648
GGTATCA4500.014.7705951
AGCGTAT801.991486E-613.0659358
GCCGGTC1352.7284841E-1112.6699988
CGTATAT853.929985E-612.29673610
TATTGGG701.0930729E-412.2107862
GTATCAA11900.012.1285551
CGCGGTC550.003062245712.09348410
TTAAGGT550.00306568512.0916724
CGGTCCA1501.4551915E-1112.03589510
CGCCGGT1353.6925485E-1011.9661097
CGTCGTA1353.6925485E-1011.96551110
CCGTCGT1459.640644E-1111.7962059
TCTACAC1459.822543E-1111.7909033
CTTGCGC1459.822543E-1111.7909033
ACACCGT658.001375E-411.6953826
TCGCGTA658.001375E-411.6953829
CGCGTAA658.00473E-411.69479810
CTACACC1157.055132E-811.5659484
CGCATCG1406.7848305E-1011.5352913
Produced by FastQC (version 0.11.5)