##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062126_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1002248
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178445853720834	32.0	32.0	32.0	32.0	32.0
2	30.70184225860266	32.0	32.0	32.0	32.0	32.0
3	30.726240411554826	32.0	32.0	32.0	32.0	32.0
4	30.725531006297842	32.0	32.0	32.0	32.0	32.0
5	30.652412376976557	32.0	32.0	32.0	32.0	32.0
6	34.267573494783726	36.0	36.0	36.0	32.0	36.0
7	34.20639302847199	36.0	36.0	36.0	32.0	36.0
8	34.164082143341766	36.0	36.0	36.0	32.0	36.0
9	34.297235813890374	36.0	36.0	36.0	32.0	36.0
10	33.98336240132183	36.0	36.0	36.0	32.0	36.0
11	34.28521882807449	36.0	36.0	36.0	32.0	36.0
12	34.09979865262889	36.0	36.0	36.0	32.0	36.0
13	34.18402930212881	36.0	36.0	36.0	32.0	36.0
14	34.081264317813556	36.0	36.0	36.0	32.0	36.0
15	34.03944532690512	36.0	36.0	36.0	32.0	36.0
16	34.04804499485157	36.0	36.0	36.0	32.0	36.0
17	33.97916084641725	36.0	36.0	36.0	32.0	36.0
18	33.9843342166809	36.0	36.0	36.0	32.0	36.0
19	33.998948364077556	36.0	36.0	36.0	32.0	36.0
20	33.98595956290259	36.0	36.0	36.0	32.0	36.0
21	33.960194482802656	36.0	36.0	36.0	32.0	36.0
22	33.93574843751247	36.0	36.0	36.0	32.0	36.0
23	33.899942928297186	36.0	36.0	36.0	32.0	36.0
24	33.88571790614698	36.0	36.0	36.0	32.0	36.0
25	33.52277779551568	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	14.0
5	91.0
6	243.0
7	52.0
8	166.0
9	127.0
10	80.0
11	25.0
12	44.0
13	33.0
14	121.0
15	187.0
16	300.0
17	426.0
18	645.0
19	870.0
20	1293.0
21	2037.0
22	3040.0
23	4554.0
24	6389.0
25	9087.0
26	12803.0
27	16430.0
28	21835.0
29	29366.0
30	38102.0
31	50778.0
32	71062.0
33	99308.0
34	208436.0
35	424304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93977983295402	17.443045022195307	11.966107884446723	25.651067260403952
2	16.806518945410716	19.265291624428286	37.10159561306184	26.82659381709916
3	19.2155994105222	22.592125861692224	28.242152851154863	29.95012187663072
4	13.095879584725365	15.497359629270397	34.768972550522285	36.637788235481956
5	15.320173836316778	35.92610559138883	33.06363551067912	15.690085061615273
6	35.110476551813655	34.39400141777409	16.425012730013876	14.070509300398374
7	30.912870323848264	30.042142926351268	19.983678231415507	19.061308518384966
8	28.402772922056517	32.788804427085935	18.992618193804876	19.81580445705267
9	27.042006447468715	14.628581247196589	18.73901737539872	39.590394929935975
10	16.854350191380245	26.508686794813002	30.438101404054603	26.19886160975215
11	36.820044383649794	21.441439461081373	21.84321041067366	19.895305744595177
12	24.53458503058805	23.549752667899583	28.160290783819452	23.755371517692915
13	29.25948608920056	19.53416530707464	24.98498870531205	26.22135989841275
14	23.650118023832523	19.543807669601197	24.875108509003873	31.930965797562404
15	25.311675102559544	26.91096452928517	21.98520904562176	25.792151322533524
16	25.93049902999135	25.942187084799333	23.28280497331727	24.844508911892046
17	24.223267411797146	26.10171150902908	24.746350605601666	24.92867047357211
18	24.90665286853887	25.30191728714956	25.56842359719589	24.223006247115677
19	25.441327312804805	25.164071387408228	25.13021340516419	24.264387894622775
20	25.671504059235172	24.503518935940086	24.811918941133353	25.01305806369139
21	26.52608955748571	24.3078754540343	24.53018622771774	24.63584876076225
22	26.064318560453216	24.16280797919305	24.850879541032597	24.921993919321135
23	24.664174186049298	24.395853055485656	25.2867950453959	25.653177713069148
24	24.77197439761593	24.843180903473737	25.19182313622214	25.193021562688187
25	25.063165736906967	24.426914496062253	25.109104454657487	25.400815312373293
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	285.0
1	285.0
2	313.0
3	341.0
4	341.0
5	341.0
6	715.0
7	1089.0
8	1089.0
9	1089.0
10	1300.0
11	1511.0
12	1511.0
13	1511.0
14	2167.5
15	2824.0
16	2824.0
17	2824.0
18	4621.0
19	6418.0
20	6418.0
21	6418.0
22	9852.0
23	13286.0
24	13286.0
25	13286.0
26	19575.0
27	25864.0
28	25864.0
29	25864.0
30	32560.5
31	39257.0
32	39257.0
33	39257.0
34	48792.5
35	58328.0
36	58328.0
37	58328.0
38	68979.5
39	79631.0
40	79631.0
41	79631.0
42	92316.0
43	105001.0
44	105001.0
45	105001.0
46	116751.5
47	128502.0
48	128502.0
49	128502.0
50	133293.5
51	138085.0
52	138085.0
53	138085.0
54	130138.5
55	122192.0
56	122192.0
57	122192.0
58	112015.0
59	101838.0
60	101838.0
61	101838.0
62	89423.5
63	77009.0
64	77009.0
65	77009.0
66	62837.0
67	48665.0
68	48665.0
69	48665.0
70	37217.5
71	25770.0
72	25770.0
73	25770.0
74	19423.0
75	13076.0
76	13076.0
77	13076.0
78	10473.0
79	7870.0
80	7870.0
81	7870.0
82	5457.5
83	3045.0
84	3045.0
85	3045.0
86	2206.5
87	1368.0
88	1368.0
89	1368.0
90	927.0
91	486.0
92	486.0
93	486.0
94	295.5
95	105.0
96	105.0
97	105.0
98	253.5
99	402.0
100	402.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.979813379522833E-4
2	0.0
3	4.988785210846018E-4
4	0.011573981689162762
5	0.03851342182773126
6	0.06764792745907201
7	0.11284632146933693
8	0.11354475139885536
9	0.12322299470789665
10	0.13579473343922863
11	0.1425794813259792
12	0.13639338766453013
13	0.1332005651295887
14	0.11763555527174911
15	0.13629361196031323
16	0.12232501336994436
17	0.12571738731331966
18	0.11434295703259074
19	0.1004741341464388
20	0.09488669471029126
21	0.09448759189342358
22	0.10416583520246486
23	0.08341248872534542
24	0.09329028344282053
25	0.09119499365426521
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1002248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.52631501239804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76568439151639	48.884492556728404
2	11.799946472103066	14.284145545998902
3	3.237219634420912	5.8781092607184044
4	1.357226711558983	3.2859172594824035
5	0.7242962096236438	2.1919490272983273
6	0.463371851770216	1.682771440087338
7	0.3033342679084088	1.2851793817435644
8	0.2209153600339928	1.0696954137995822
9	0.16086909364596516	0.8763132093997184
>10	0.8417976112003406	9.096524141625935
>50	0.06631499637218576	2.8080205619928305
>100	0.05422878239939278	6.522607436619879
>500	0.004298622538249424	1.7234909165915506
>1k	4.95994908259549E-4	0.4107838479131577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1842	0.1837868471675673	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1158	0.11554026548319378	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1105	0.110252153159697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.977570421692036E-5
2	0.0	0.0	0.0	0.0	9.977570421692036E-5
3	0.0	0.0	0.0	0.0	9.977570421692036E-5
4	0.0	0.0	0.0	0.0	9.977570421692036E-5
5	0.0	0.0	0.0	0.0	9.977570421692036E-5
6	0.0	0.0	0.0	0.0	9.977570421692036E-5
7	0.0	0.0	0.0	0.0	9.977570421692036E-5
8	0.0	0.0	0.0	0.0	9.977570421692036E-5
9	0.0	0.0	0.0	0.0	9.977570421692036E-5
10	0.0	0.0	0.0	0.0	9.977570421692036E-5
11	0.0	0.0	0.0	0.0	9.977570421692036E-5
12	0.0	0.0	0.0	0.0	2.9932711265076106E-4
13	0.0	0.0	0.0	0.0	2.9932711265076106E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGAAC	45	6.745893E-4	14.781664	8
GGTATCA	450	0.0	14.770595	1
AGCGTAT	80	1.991486E-6	13.065935	8
GCCGGTC	135	2.7284841E-11	12.669998	8
CGTATAT	85	3.929985E-6	12.296736	10
TATTGGG	70	1.0930729E-4	12.210786	2
GTATCAA	1190	0.0	12.128555	1
CGCGGTC	55	0.0030622457	12.093484	10
TTAAGGT	55	0.003065685	12.091672	4
CGGTCCA	150	1.4551915E-11	12.035895	10
CGCCGGT	135	3.6925485E-10	11.966109	7
CGTCGTA	135	3.6925485E-10	11.965511	10
CCGTCGT	145	9.640644E-11	11.796205	9
TCTACAC	145	9.822543E-11	11.790903	3
CTTGCGC	145	9.822543E-11	11.790903	3
ACACCGT	65	8.001375E-4	11.695382	6
TCGCGTA	65	8.001375E-4	11.695382	9
CGCGTAA	65	8.00473E-4	11.694798	10
CTACACC	115	7.055132E-8	11.565948	4
CGCATCG	140	6.7848305E-10	11.53529	13
>>END_MODULE