FastQCFastQC Report
Thu 2 Feb 2017
SRR4062126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002248
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11500.025.0681741
GGACCGT801.3094541E-724.7498056
GGTATCA5900.023.1253851
CCGTGTA654.9316173E-420.3075319
TTGTTCG654.9316173E-420.30753136
TACTAGT550.00448329219.9998417
ATAAGTC600.00741157918.3331893
ACTATAC750.0012907317.5998613
GTATTAC1408.3400664E-817.2907521
GTCCTAA1154.2595675E-617.222411
GTCTTAG1351.081693E-616.3010481
GACTATA1351.081693E-616.3010481
TATACTG1351.0850799E-616.2961675
CGTCGTA2301.8189894E-1216.2607410
ATACTGT2353.6379788E-1215.9147666
ATCAACG18700.015.7645813
TCAACGC18650.015.6888844
TATCAAC19050.015.5912062
ATATACC850.002979623615.5292873
CAACGCA18900.015.4813585
Produced by FastQC (version 0.11.5)