##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062126_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1002248
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259717155833687	32.0	32.0	32.0	32.0	32.0
2	31.40517416846928	32.0	32.0	32.0	32.0	32.0
3	31.527849394561027	32.0	32.0	32.0	32.0	32.0
4	31.616603874490146	32.0	32.0	32.0	32.0	32.0
5	31.59261280641119	32.0	32.0	32.0	32.0	32.0
6	35.21418451321429	36.0	36.0	36.0	36.0	36.0
7	35.211361858542	36.0	36.0	36.0	36.0	36.0
8	35.15956629496891	36.0	36.0	36.0	36.0	36.0
9	35.253793472274324	36.0	36.0	36.0	36.0	36.0
10	35.13156623909452	36.0	36.0	36.0	36.0	36.0
11	35.251988529785045	36.0	36.0	36.0	36.0	36.0
12	35.16131636082088	36.0	36.0	36.0	36.0	36.0
13	35.201618761025216	36.0	36.0	36.0	36.0	36.0
14	35.16151990325748	36.0	36.0	36.0	36.0	36.0
15	35.13762661536865	36.0	36.0	36.0	36.0	36.0
16	35.164066179229096	36.0	36.0	36.0	36.0	36.0
17	35.135343747256165	36.0	36.0	36.0	36.0	36.0
18	35.140122005731115	36.0	36.0	36.0	36.0	36.0
19	35.124244697919075	36.0	36.0	36.0	36.0	36.0
20	35.12223521523615	36.0	36.0	36.0	36.0	36.0
21	35.11484283331072	36.0	36.0	36.0	36.0	36.0
22	35.10043921264996	36.0	36.0	36.0	36.0	36.0
23	35.06771278166681	36.0	36.0	36.0	36.0	36.0
24	35.05162893814705	36.0	36.0	36.0	36.0	36.0
25	35.03851142631365	36.0	36.0	36.0	36.0	36.0
26	34.99425591270823	36.0	36.0	36.0	36.0	36.0
27	34.97779591478356	36.0	36.0	36.0	36.0	36.0
28	34.959664673813265	36.0	36.0	36.0	36.0	36.0
29	34.93677512950887	36.0	36.0	36.0	32.0	36.0
30	34.914884340003674	36.0	36.0	36.0	32.0	36.0
31	34.92718768209066	36.0	36.0	36.0	32.0	36.0
32	34.89616442237849	36.0	36.0	36.0	32.0	36.0
33	34.88433601264358	36.0	36.0	36.0	32.0	36.0
34	34.86334420223338	36.0	36.0	36.0	32.0	36.0
35	34.85103287809005	36.0	36.0	36.0	32.0	36.0
36	34.82285921249032	36.0	36.0	36.0	32.0	36.0
37	34.82370032167687	36.0	36.0	36.0	32.0	36.0
38	34.8081013880796	36.0	36.0	36.0	32.0	36.0
39	34.786748389620136	36.0	36.0	36.0	32.0	36.0
40	34.78033380959603	36.0	36.0	36.0	32.0	36.0
41	34.756608144890286	36.0	36.0	36.0	32.0	36.0
42	34.71606029645357	36.0	36.0	36.0	32.0	36.0
43	34.72260159162203	36.0	36.0	36.0	32.0	36.0
44	34.67306993877763	36.0	36.0	36.0	32.0	36.0
45	34.66032159704983	36.0	36.0	36.0	32.0	36.0
46	34.64768500411076	36.0	36.0	36.0	32.0	36.0
47	34.616682697296476	36.0	36.0	36.0	32.0	36.0
48	34.59485277097086	36.0	36.0	36.0	32.0	36.0
49	34.58157661576775	36.0	36.0	36.0	32.0	36.0
50	34.15513026715943	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	8.0
21	22.0
22	66.0
23	194.0
24	475.0
25	1126.0
26	2398.0
27	4609.0
28	8120.0
29	13241.0
30	19990.0
31	29621.0
32	45110.0
33	76250.0
34	170244.0
35	630770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.526697047545476	17.98410293568715	12.418034504853555	26.07116551191381
2	16.245826689629435	19.844602185986457	36.793380975092845	27.11619014929126
3	18.427992301298982	23.189493208759917	28.56451553359601	29.817998956345093
4	12.822413681285513	15.955479947517823	35.03384900897485	36.18825736222181
5	14.948296230074792	36.28313551137044	32.848057566590306	15.920510691964465
6	35.13775088700955	34.659823356388266	16.24464701210484	13.957778744497348
7	30.955611784707976	30.15361467421237	19.903556804303925	18.98721673677573
8	28.48117432012835	32.925683064471066	18.81201060017082	19.781132015229762
9	27.113448966722807	14.549093637502894	18.64179324877675	39.69566414699755
10	16.740966307740198	26.52946176994117	30.63862437241082	26.090947549907806
11	37.21354395319322	21.268189110878744	21.760382659780813	19.757884276147223
12	24.660962157070905	23.416559574077475	28.222056816277007	23.700421452574613
13	29.380851845052327	19.437803817019343	25.06844613309281	26.112898204835528
14	23.77704919341321	19.296820746960833	24.843451920083652	32.08267813954231
15	25.315590552438117	26.968774195608276	21.962927339341164	25.75270791261245
16	25.922330290827382	25.93230790107	23.241446194739005	24.903915613363612
17	24.2917920514683	26.04744534286923	24.824294984874005	24.83646762078847
18	25.016487951572813	25.15487699140032	25.550986932362978	24.27764812466388
19	25.611126188328637	24.987927139789754	25.260514363710378	24.14043230817123
20	25.93186076815779	24.291338818369216	24.76118542228324	25.015614991189754
21	26.779499684708775	24.207182254292352	24.490545254268405	24.52277280673047
22	26.129860074552408	24.07448056768385	24.935046016554786	24.86061334120896
23	24.75574907607698	24.130354962045324	25.28575761687726	25.82813834500044
24	24.82144959580459	24.740031808811878	25.187630083872293	25.25088851151124
25	25.26420711922446	24.302864372348452	25.07124013713228	25.361688371294814
26	24.823546314976564	25.04265419866979	25.48705607206215	24.646743414291503
27	25.347768911025838	24.64843960464796	24.877724630479943	25.12606685384626
28	25.036543325667132	24.51770293394264	25.321705987118794	25.12404775327144
29	24.780942004258435	24.900772864147125	25.29748185575198	25.020803275842457
30	24.84963621342678	24.962483886030835	25.34133677098009	24.8465431295623
31	25.392094747292326	24.64596979780393	24.650060613921745	25.311874840981996
32	25.233003305598768	24.701493356375074	24.599022787977717	25.46648055004844
33	24.911174413441824	24.44971040015166	25.081092946335477	25.558022240071043
34	25.522155892955674	24.55172867948663	25.16006661085829	24.76604881669941
35	25.77251801461124	24.421347339265367	25.24649735293472	24.559637293188672
36	24.770914068829548	24.95899202190285	24.916287850064457	25.353806059203148
37	25.66022900976624	24.692185810015285	24.72291734016611	24.924667840052365
38	25.01646299119579	24.467197739481644	25.2553260270911	25.26101324223146
39	25.61205460972882	24.51830351842225	24.60660580959232	25.263036062256607
40	25.713895163672063	24.584434191936527	24.953404746130698	24.74826589826071
41	24.736039687338483	25.054727738232792	25.274935793437457	24.934296780991268
42	25.82276081240864	24.966947879924767	24.77187800899018	24.438413298676416
43	25.468741425157525	24.263598764723785	24.954375601798034	25.313284208320653
44	25.015465789673243	24.781283800233883	24.834266602275765	25.36898380781711
45	25.177030752514153	24.98485871963138	24.78320779623778	25.05490273161669
46	25.08418801841111	24.797424919254336	24.78315660917103	25.335230453163526
47	25.24961561141482	24.691167094541132	25.10952954429124	24.949687749752805
48	25.828506428389687	25.214554053851796	24.197586298075723	24.759353219682797
49	25.149444876268323	25.231563476280893	24.451386886368013	25.167604761082767
50	25.357752212565977	25.27623399818405	24.39270426947906	24.97330951977091
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	73.0
2	40.0
3	89.0
4	138.0
5	156.5
6	175.0
7	139.0
8	103.0
9	143.0
10	183.0
11	299.0
12	415.0
13	795.0
14	1175.0
15	1481.0
16	1787.0
17	1787.0
18	1787.0
19	1796.0
20	1805.0
21	2151.0
22	2497.0
23	3119.0
24	3741.0
25	4824.5
26	5908.0
27	7245.5
28	8583.0
29	10189.0
30	11795.0
31	13343.5
32	14892.0
33	17014.0
34	19136.0
35	22153.0
36	25170.0
37	29803.0
38	34436.0
39	38497.5
40	42559.0
41	46252.0
42	49945.0
43	52321.0
44	54697.0
45	59803.5
46	64910.0
47	69193.5
48	73477.0
49	76424.0
50	79371.0
51	78051.0
52	76731.0
53	76021.0
54	75311.0
55	75448.0
56	75585.0
57	72949.5
58	70314.0
59	64160.5
60	58007.0
61	51346.0
62	44685.0
63	38602.5
64	32520.0
65	27309.5
66	22099.0
67	18889.0
68	15679.0
69	13859.5
70	12040.0
71	9543.5
72	7047.0
73	6286.0
74	5525.0
75	4066.5
76	2608.0
77	2358.5
78	2109.0
79	1792.5
80	1476.0
81	1126.5
82	777.0
83	618.5
84	460.0
85	365.0
86	270.0
87	170.5
88	71.0
89	53.5
90	36.0
91	32.0
92	28.0
93	18.0
94	8.0
95	10.0
96	12.0
97	13.5
98	15.0
99	9.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03013226267350995
2	0.004589682393978336
3	9.977570421692036E-5
4	2.993271126507611E-4
5	0.0
6	3.9910281686768144E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.9910281686768144E-4
17	0.0
18	9.977570421692036E-5
19	0.0
20	5.986542253015222E-4
21	0.0
22	0.0
23	0.0
24	9.977570421692036E-4
25	3.9910281686768144E-4
26	1.9955140843384072E-4
27	1.9955140843384072E-4
28	0.0012970841548199648
29	1.9955140843384072E-4
30	0.0011973084506030445
31	2.993271126507611E-4
32	8.979813379522833E-4
33	2.993271126507611E-4
34	8.979813379522833E-4
35	5.986542253015222E-4
36	3.9910281686768144E-4
37	0.0019955140843384072
38	0.0
39	8.979813379522833E-4
40	0.0
41	0.001396859859036885
42	0.004290355281327576
43	0.004290355281327576
44	0.003591925351809133
45	0.0020952897885553276
46	0.00269394401385685
47	6.984299295184426E-4
48	0.005288112323496779
49	0.003891252464459894
50	0.0017959626759045666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1002248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.87093321139895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.64041180259662	49.89297532778163
2	12.03041940568935	14.886665511090474
3	3.2522163052516295	6.036529734337386
4	1.309095779265172	3.239799101049589
5	0.7354344772106425	2.2751008710429885
6	0.4369734085308543	1.62215715446219
7	0.3056289769133177	1.323668501265045
8	0.21036270797008993	1.0412269643989165
9	0.15238346970524755	0.8485296728989147
>10	0.8163802852613956	8.872644486990438
>50	0.05948789164643973	2.561812932095599
>100	0.047478508592703424	5.803595410525532
>500	0.003726981366672292	1.5952943320613109
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.9955140843384072E-4
13	0.0	0.0	0.0	0.0	2.9932711265076106E-4
14	0.0	0.0	0.0	0.0	2.9932711265076106E-4
15	0.0	0.0	0.0	0.0	3.9910281686768144E-4
16	0.0	0.0	0.0	0.0	3.9910281686768144E-4
17	0.0	0.0	0.0	9.977570421692036E-5	3.9910281686768144E-4
18	0.0	0.0	0.0	9.977570421692036E-5	3.9910281686768144E-4
19	0.0	9.977570421692036E-5	0.0	9.977570421692036E-5	3.9910281686768144E-4
20	0.0	9.977570421692036E-5	0.0	9.977570421692036E-5	3.9910281686768144E-4
21	0.0	9.977570421692036E-5	0.0	1.9955140843384072E-4	4.988785210846018E-4
22	0.0	9.977570421692036E-5	0.0	1.9955140843384072E-4	4.988785210846018E-4
23	0.0	9.977570421692036E-5	0.0	2.9932711265076106E-4	4.988785210846018E-4
24	0.0	9.977570421692036E-5	0.0	2.9932711265076106E-4	4.988785210846018E-4
25	0.0	9.977570421692036E-5	0.0	2.9932711265076106E-4	4.988785210846018E-4
26	0.0	9.977570421692036E-5	0.0	3.9910281686768144E-4	4.988785210846018E-4
27	0.0	9.977570421692036E-5	0.0	4.988785210846018E-4	4.988785210846018E-4
28	0.0	9.977570421692036E-5	0.0	5.986542253015221E-4	4.988785210846018E-4
29	0.0	9.977570421692036E-5	0.0	0.0014966355632538054	4.988785210846018E-4
30	0.0	9.977570421692036E-5	0.0	0.002993271126507611	4.988785210846018E-4
31	0.0	9.977570421692036E-5	0.0	0.005288112323496779	4.988785210846018E-4
32	0.0	9.977570421692036E-5	0.0	0.007483177816269027	4.988785210846018E-4
33	0.0	9.977570421692036E-5	0.0	0.009877794717475116	5.986542253015221E-4
34	0.0	9.977570421692036E-5	0.0	0.011973084506030443	5.986542253015221E-4
35	0.0	9.977570421692036E-5	0.0	0.015165907040971895	5.986542253015221E-4
36	0.0	9.977570421692036E-5	0.0	0.021252224998204037	5.986542253015221E-4
37	0.0	9.977570421692036E-5	0.0	0.029134505631340745	5.986542253015221E-4
38	0.0	9.977570421692036E-5	0.0	0.040010057390985065	5.986542253015221E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1150	0.0	25.068174	1
GGACCGT	80	1.3094541E-7	24.749805	6
GGTATCA	590	0.0	23.125385	1
CCGTGTA	65	4.9316173E-4	20.307531	9
TTGTTCG	65	4.9316173E-4	20.307531	36
TACTAGT	55	0.004483292	19.99984	17
ATAAGTC	60	0.007411579	18.333189	3
ACTATAC	75	0.00129073	17.599861	3
GTATTAC	140	8.3400664E-8	17.290752	1
GTCCTAA	115	4.2595675E-6	17.22241	1
GTCTTAG	135	1.081693E-6	16.301048	1
GACTATA	135	1.081693E-6	16.301048	1
TATACTG	135	1.0850799E-6	16.296167	5
CGTCGTA	230	1.8189894E-12	16.26074	10
ATACTGT	235	3.6379788E-12	15.914766	6
ATCAACG	1870	0.0	15.764581	3
TCAACGC	1865	0.0	15.688884	4
TATCAAC	1905	0.0	15.591206	2
ATATACC	85	0.0029796236	15.529287	3
CAACGCA	1890	0.0	15.481358	5
>>END_MODULE