FastQCFastQC Report
Thu 2 Feb 2017
SRR4062125_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062125_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731506
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22490.3074479225050786No Hit
TATCAACGCAGAGTACTTTTTTTTT13320.18209009905592025No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9350.1278185004634275No Hit
GGTATCAACGCAGAGTACTTTTTTT7680.10498888594215221No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7340.10034094047075486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT551.965168E-413.812161
TAAGACT852.6954694E-713.4105094
CTAAGAC604.0906444E-412.6654813
CAAGACG604.0906444E-412.6654814
TCTTATA701.0903282E-412.2131412
AGTACAG1209.957148E-911.8763275
TAATACC802.8642356E-511.8738884
TTAGACA1052.715351E-711.7608044
TTGGACA658.020794E-411.6912134
GTATTGG1800.011.6060511
GTATCAA9950.011.4522931
GTACCGT752.0687997E-411.4012746
ACCGTGC855.307633E-511.1777198
TTAAGAC855.3187498E-511.1754243
GCGTAAC600.005865726611.08457111
CTAGAAC600.005874701311.0822953
TTGGACC600.005874701311.0822954
GCTATAC600.005874701311.0822953
ATTAAGT600.005874701311.0822953
CAGTACT600.005874701311.0822954