##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062125_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731506
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.190281419427865	32.0	32.0	32.0	32.0	32.0
2	30.79721013908293	32.0	32.0	32.0	32.0	32.0
3	30.816428026564374	32.0	32.0	32.0	32.0	32.0
4	30.823255038236187	32.0	32.0	32.0	32.0	32.0
5	30.789993520217195	32.0	32.0	32.0	32.0	32.0
6	34.394227798541635	36.0	36.0	36.0	32.0	36.0
7	34.32793305864887	36.0	36.0	36.0	32.0	36.0
8	34.2786320276252	36.0	36.0	36.0	32.0	36.0
9	34.37968109625895	36.0	36.0	36.0	32.0	36.0
10	34.1313974184764	36.0	36.0	36.0	32.0	36.0
11	34.38764548752847	36.0	36.0	36.0	32.0	36.0
12	34.237078028068126	36.0	36.0	36.0	32.0	36.0
13	34.30971994761492	36.0	36.0	36.0	32.0	36.0
14	34.23061191569174	36.0	36.0	36.0	32.0	36.0
15	34.188997766252086	36.0	36.0	36.0	32.0	36.0
16	34.19402438257512	36.0	36.0	36.0	32.0	36.0
17	34.138272276645715	36.0	36.0	36.0	32.0	36.0
18	34.143208668144894	36.0	36.0	36.0	32.0	36.0
19	34.13061820408855	36.0	36.0	36.0	32.0	36.0
20	34.10744819591363	36.0	36.0	36.0	32.0	36.0
21	34.08120644259924	36.0	36.0	36.0	32.0	36.0
22	34.06890442457068	36.0	36.0	36.0	32.0	36.0
23	34.04338720393271	36.0	36.0	36.0	32.0	36.0
24	34.01152553772628	36.0	36.0	36.0	32.0	36.0
25	33.65119766618456	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	15.0
5	58.0
6	154.0
7	38.0
8	117.0
9	108.0
10	59.0
11	11.0
12	32.0
13	44.0
14	143.0
15	219.0
16	300.0
17	403.0
18	534.0
19	730.0
20	1086.0
21	1533.0
22	2268.0
23	3239.0
24	4297.0
25	5909.0
26	8262.0
27	10664.0
28	13999.0
29	18854.0
30	24722.0
31	33643.0
32	48738.0
33	69343.0
34	151436.0
35	330548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56101468770848	16.83795946936142	11.334580093397639	26.266445749532462
2	16.364093702836897	19.691348235963165	37.57641790065399	26.368140160545945
3	19.4094897765286	23.3196582925839	28.047595156820954	29.22325677406655
4	12.929519965765552	15.53934979157182	35.38493930358054	36.146190939082096
5	14.691064937764441	36.3081774595421	33.8009774989265	15.199780103766955
6	34.24632237272277	35.0477811124395	16.775233160338257	13.930663354499467
7	30.042233012366086	30.450193510951024	20.442639193768237	19.064934282914653
8	27.7018094630731	33.36216358246865	19.397876597410203	19.538150357048035
9	27.18001990017067	14.693826156817178	18.488955679007784	39.63719826400437
10	15.515818205960418	27.294977954029942	32.30949503024562	24.87970880976402
11	36.251730024463335	21.70419216926929	22.45041281018945	19.593664996077926
12	24.30958889483576	23.718658586831985	29.26193256249119	22.709819955841066
13	29.46913992564893	20.23205965605786	24.957020920135562	25.341779498157646
14	23.192297427685737	19.44092025757358	25.795953001717614	31.57082931302307
15	24.86109342981163	27.004340701986372	22.765072338188734	25.369493530013266
16	25.05827043228928	26.177555447583984	24.050530695481388	24.713643424645348
17	23.633200659173408	25.962891384209403	25.636863341965366	24.767044614651827
18	23.81081417675646	25.833015140504372	26.199381679483334	24.15678900325583
19	24.953508516254573	25.326261427873543	25.992536731351063	23.727693324520818
20	25.156982273215522	25.033695258033838	25.698979905995362	24.110342562755278
21	25.365383747008448	24.81831728486454	25.33692206013352	24.47937690799349
22	25.05610842885451	24.800062403520872	25.62937235195586	24.51445681566876
23	24.321872733989135	24.835752691849887	25.67566458250674	25.16670999165424
24	24.092804833995597	25.163514060840914	25.962612065285214	24.781069039878275
25	24.476316750885633	24.82044461593825	25.902908051497402	24.800330581678715
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	846.0
1	846.0
2	637.5
3	429.0
4	429.0
5	429.0
6	960.5
7	1492.0
8	1492.0
9	1492.0
10	1557.5
11	1623.0
12	1623.0
13	1623.0
14	1937.0
15	2251.0
16	2251.0
17	2251.0
18	3737.5
19	5224.0
20	5224.0
21	5224.0
22	8000.0
23	10776.0
24	10776.0
25	10776.0
26	15377.0
27	19978.0
28	19978.0
29	19978.0
30	25594.0
31	31210.0
32	31210.0
33	31210.0
34	38045.0
35	44880.0
36	44880.0
37	44880.0
38	53588.0
39	62296.0
40	62296.0
41	62296.0
42	70368.0
43	78440.0
44	78440.0
45	78440.0
46	86824.5
47	95209.0
48	95209.0
49	95209.0
50	98189.0
51	101169.0
52	101169.0
53	101169.0
54	95475.5
55	89782.0
56	89782.0
57	89782.0
58	80977.5
59	72173.0
60	72173.0
61	72173.0
62	62156.5
63	52140.0
64	52140.0
65	52140.0
66	42147.0
67	32154.0
68	32154.0
69	32154.0
70	24175.0
71	16196.0
72	16196.0
73	16196.0
74	11812.5
75	7429.0
76	7429.0
77	7429.0
78	5407.5
79	3386.0
80	3386.0
81	3386.0
82	2386.0
83	1386.0
84	1386.0
85	1386.0
86	959.0
87	532.0
88	532.0
89	532.0
90	351.5
91	171.0
92	171.0
93	171.0
94	113.0
95	55.0
96	55.0
97	55.0
98	167.0
99	279.0
100	279.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001367042785705107
2	2.734085571410214E-4
3	4.1011283571153206E-4
4	0.010799638007070345
5	0.03444947819976869
6	0.06343078525671696
7	0.10909001429926753
8	0.10826978862784448
9	0.1174289752920687
10	0.13492712294909406
11	0.14012188553477348
12	0.1316462202634018
13	0.12549452772772884
14	0.1149682982777995
15	0.13383348872052997
16	0.11770238384920971
17	0.12221362504203656
18	0.11168739559210723
19	0.09938401052076128
20	0.0944626564922229
21	0.09555629072078697
22	0.10635592872785733
23	0.08147575002802439
24	0.0932323179850883
25	0.0920019794779537
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	731506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.21758136890946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25353796519506	47.4431070301675
2	14.167021974194782	17.628756848691843
3	4.374554152584614	8.165225368234019
4	1.8634198621874543	4.6374990760036
5	0.953704786081589	2.9668602564974824
6	0.5672549249093376	2.11759376684768
7	0.38570751454944546	1.6798452069755967
8	0.271761496058816	1.352667439518076
9	0.19299417540727304	1.0806867730914835
>10	0.9248008751308	9.631381321620147
>50	0.035311245763104515	1.4737548319801503
>100	0.008386201371420186	0.8124495144974035
>500	0.0011034475473113473	0.5184737812619357
>1k	4.4137901892453896E-4	0.49169878461308036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2249	0.3074479225050786	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1332	0.18209009905592025	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	935	0.1278185004634275	No Hit
GGTATCAACGCAGAGTACTTTTTTT	768	0.10498888594215221	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	734	0.10034094047075486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.367042785705107E-4
2	0.0	0.0	0.0	0.0	1.367042785705107E-4
3	0.0	0.0	0.0	0.0	1.367042785705107E-4
4	0.0	0.0	0.0	0.0	1.367042785705107E-4
5	0.0	0.0	0.0	0.0	1.367042785705107E-4
6	0.0	0.0	0.0	0.0	1.367042785705107E-4
7	0.0	0.0	0.0	0.0	1.367042785705107E-4
8	0.0	0.0	0.0	0.0	1.367042785705107E-4
9	0.0	0.0	0.0	1.367042785705107E-4	1.367042785705107E-4
10	0.0	0.0	0.0	1.367042785705107E-4	2.734085571410214E-4
11	0.0	0.0	0.0	2.734085571410214E-4	2.734085571410214E-4
12	0.0	0.0	0.0	2.734085571410214E-4	4.101128357115321E-4
13	0.0	0.0	0.0	2.734085571410214E-4	4.101128357115321E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	55	1.965168E-4	13.81216	1
TAAGACT	85	2.6954694E-7	13.410509	4
CTAAGAC	60	4.0906444E-4	12.665481	3
CAAGACG	60	4.0906444E-4	12.665481	4
TCTTATA	70	1.0903282E-4	12.213141	2
AGTACAG	120	9.957148E-9	11.876327	5
TAATACC	80	2.8642356E-5	11.873888	4
TTAGACA	105	2.715351E-7	11.760804	4
TTGGACA	65	8.020794E-4	11.691213	4
GTATTGG	180	0.0	11.606051	1
GTATCAA	995	0.0	11.452293	1
GTACCGT	75	2.0687997E-4	11.401274	6
ACCGTGC	85	5.307633E-5	11.177719	8
TTAAGAC	85	5.3187498E-5	11.175424	3
GCGTAAC	60	0.0058657266	11.084571	11
CTAGAAC	60	0.0058747013	11.082295	3
TTGGACC	60	0.0058747013	11.082295	4
GCTATAC	60	0.0058747013	11.082295	3
ATTAAGT	60	0.0058747013	11.082295	3
CAGTACT	60	0.0058747013	11.082295	4
>>END_MODULE