FastQCFastQC Report
Thu 2 Feb 2017
SRR4062125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731506
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.12125669509204298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA500.002578240622.0018137
ATAGGGG500.002580288321.9988023
TTCGTAT550.004481584820.0002825
CCGCTAG550.004481584820.0002836
ATTGCGC550.00448335819.99891330
GTATCAA14200.019.059211
AGGCGTT600.00740293518.33609642
GCTTAAC600.00740293518.3360961
ATAATAC600.00741169818.3323363
TAACGGA600.00741169818.3323364
TATTGCG600.00741169818.33233629
ACTTATC851.4304499E-418.1166638
GTCCTAT1102.7893439E-618.0027141
AAATCCG750.001290680117.59904329
ACGCTCC750.001290680117.59904335
GTTATAG902.2113182E-417.1136911
TAAGACT1551.4373654E-817.0313324
CTATAAC1053.7728154E-516.7609943
ATTCGTA800.001986246316.50135824
GCATTCG800.001986246316.50135822
Produced by FastQC (version 0.11.5)