##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731506
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19535041407726	32.0	32.0	32.0	32.0	32.0
2	31.384528629977062	32.0	32.0	32.0	32.0	32.0
3	31.529634753508514	32.0	32.0	32.0	32.0	32.0
4	31.622808288653818	32.0	32.0	32.0	32.0	32.0
5	31.592191998425168	32.0	32.0	32.0	32.0	32.0
6	35.21442886319456	36.0	36.0	36.0	36.0	36.0
7	35.22723668705383	36.0	36.0	36.0	36.0	36.0
8	35.171327371204065	36.0	36.0	36.0	36.0	36.0
9	35.254953479534	36.0	36.0	36.0	36.0	36.0
10	35.13295994838047	36.0	36.0	36.0	36.0	36.0
11	35.256737470369345	36.0	36.0	36.0	36.0	36.0
12	35.18905518204909	36.0	36.0	36.0	36.0	36.0
13	35.21983141628367	36.0	36.0	36.0	36.0	36.0
14	35.181038843153715	36.0	36.0	36.0	36.0	36.0
15	35.15630493803195	36.0	36.0	36.0	36.0	36.0
16	35.1678906256408	36.0	36.0	36.0	36.0	36.0
17	35.15387433595897	36.0	36.0	36.0	36.0	36.0
18	35.14797281225308	36.0	36.0	36.0	36.0	36.0
19	35.13674802393965	36.0	36.0	36.0	36.0	36.0
20	35.12807687154993	36.0	36.0	36.0	36.0	36.0
21	35.12641318047972	36.0	36.0	36.0	36.0	36.0
22	35.105609523366866	36.0	36.0	36.0	36.0	36.0
23	35.07878404278297	36.0	36.0	36.0	36.0	36.0
24	35.066811482065766	36.0	36.0	36.0	36.0	36.0
25	35.05054777404423	36.0	36.0	36.0	36.0	36.0
26	35.001198896523064	36.0	36.0	36.0	36.0	36.0
27	34.98664535902645	36.0	36.0	36.0	36.0	36.0
28	34.96162437491969	36.0	36.0	36.0	36.0	36.0
29	34.93890275677848	36.0	36.0	36.0	36.0	36.0
30	34.931740819624174	36.0	36.0	36.0	36.0	36.0
31	34.922932962955876	36.0	36.0	36.0	36.0	36.0
32	34.892307103427726	36.0	36.0	36.0	32.0	36.0
33	34.869768669019805	36.0	36.0	36.0	32.0	36.0
34	34.8470661894776	36.0	36.0	36.0	32.0	36.0
35	34.839814027499436	36.0	36.0	36.0	32.0	36.0
36	34.81225991311076	36.0	36.0	36.0	32.0	36.0
37	34.784683926037516	36.0	36.0	36.0	32.0	36.0
38	34.781326468955825	36.0	36.0	36.0	32.0	36.0
39	34.75564793726914	36.0	36.0	36.0	32.0	36.0
40	34.73541980516906	36.0	36.0	36.0	32.0	36.0
41	34.700074913944654	36.0	36.0	36.0	32.0	36.0
42	34.68077363685329	36.0	36.0	36.0	32.0	36.0
43	34.6694299158175	36.0	36.0	36.0	32.0	36.0
44	34.63696128261422	36.0	36.0	36.0	32.0	36.0
45	34.591170817464246	36.0	36.0	36.0	32.0	36.0
46	34.56218951040729	36.0	36.0	36.0	32.0	36.0
47	34.527041473344035	36.0	36.0	36.0	32.0	36.0
48	34.50841278130323	36.0	36.0	36.0	32.0	36.0
49	34.48221340631519	36.0	36.0	36.0	32.0	36.0
50	34.056421956894404	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	3.0
21	22.0
22	65.0
23	137.0
24	397.0
25	959.0
26	1947.0
27	3760.0
28	6521.0
29	9959.0
30	15058.0
31	21890.0
32	32993.0
33	54382.0
34	121502.0
35	461908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.968591714163814	17.461902000897066	11.865078930958658	26.70442735398046
2	15.89235936899754	20.175235928697106	37.25957693469734	26.67282776760801
3	18.719924976486798	23.646350532601325	28.472298169251296	29.161426321660578
4	12.629715449655162	15.948489757417928	35.57405622654664	35.84773856638027
5	14.41439224612272	36.64021435260183	33.64652326368241	15.298870137593045
6	34.440639503489386	35.19497474385001	16.645409122288978	13.718976630371632
7	30.15887771255465	30.340147585939143	20.538040699597815	18.96293400190839
8	27.831624074170275	33.285031154905084	19.379061825877024	19.504282945047613
9	27.263070979595522	14.444857595153012	18.330266600684066	39.9618048245674
10	15.438014178967773	27.309687138588064	32.41203763195381	24.84026105049036
11	36.521778358619066	21.450678463334548	22.50439504255604	19.523148135490345
12	24.26596637621564	23.611836403255747	29.34904156630294	22.773155654225665
13	29.58171224842995	20.00885843725137	25.144564774588314	25.264864539730365
14	23.29864690105071	19.066419140786266	26.07784488438919	31.55708907377383
15	24.976008399110874	26.819192187077075	22.909039707124755	25.2957597066873
16	25.193779374492483	26.017973976831232	24.014151704301558	24.774094944374724
17	23.78859503544742	25.78844192665542	25.7273351141344	24.695627923762757
18	23.997648683194235	25.61745989432745	26.368650931982696	24.016240490495623
19	25.063225728838862	25.16630075488103	26.050914141510802	23.71955937476931
20	25.40361938247943	24.734725347433926	25.89452444682614	23.967130823260508
21	25.483591385443184	24.478541529392785	25.559325555771245	24.478541529392785
22	24.960970928468118	24.68578521570568	25.806213482869587	24.547030372956613
23	24.45205432635457	24.69374782127258	25.72819051134305	25.126007341029794
24	24.28116981384851	24.91165425611175	26.11589047725157	24.691285452788172
25	24.776795173772356	24.45731596452783	26.047466654272096	24.71842220742772
26	24.430318316347805	24.85875011107238	26.035228740746817	24.675702831833
27	24.431068635398624	25.216848051204167	25.866469447151957	24.485613866245252
28	24.505600866172518	24.895487864562117	25.972461838374617	24.62644943089075
29	24.384762394293418	25.20430454432363	25.749891320098538	24.661041741284418
30	24.60164046479836	25.149555707450443	25.75816814764183	24.490635680109367
31	24.60403389191007	25.044224075942378	25.64982734155204	24.701914690595515
32	24.366543951289334	25.273307149799795	25.6927175965102	24.667431302400676
33	24.48742730749987	24.976555216225158	25.859391447233516	24.676626029041458
34	24.51271493447728	25.038072349768008	26.035198810119454	24.414013905635255
35	24.691833958756263	24.912338261529314	25.89838757083	24.497440208884424
36	24.665073601784815	25.17689582011855	25.736838076073404	24.421192502023228
37	24.975529599615033	24.872863282916104	25.5440904666601	24.60751665080875
38	24.617843189256135	25.22139257914494	25.615647718542295	24.545116513056627
39	24.771565276828433	25.31414900888585	25.445386192754615	24.4688995215311
40	24.765927779030903	25.15963663953083	25.679113608246013	24.395321973192257
41	24.41985883858303	25.18663628173774	25.750953858015617	24.642551021663618
42	24.630334448892096	25.265902180808013	25.62299035369775	24.48077301660214
43	24.54623129660895	24.87706514829422	25.711395312275716	24.86530824282111
44	24.37359530592938	25.190846999764858	25.783621062071756	24.651936632234005
45	24.589334733204282	25.368286361101084	25.327137810733074	24.715241094961556
46	24.60689824877989	25.3028749538613	25.380934804303546	24.70929199305527
47	24.513019192149795	25.465312913108324	25.598601224611993	24.423066670129884
48	24.62411514284855	25.55237780153498	25.23671381347799	24.586793242138477
49	24.64845149136406	25.246555603859584	25.344439614041708	24.76055329073465
50	24.484411355390474	25.684831317823527	25.19214101551072	24.63861631127528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	522.0
1	300.0
2	78.0
3	247.0
4	416.0
5	384.0
6	352.0
7	270.0
8	188.0
9	270.0
10	352.0
11	557.0
12	762.0
13	1261.0
14	1760.0
15	2142.0
16	2524.0
17	2373.5
18	2223.0
19	2155.5
20	2088.0
21	2344.0
22	2600.0
23	2957.5
24	3315.0
25	3964.5
26	4614.0
27	5734.0
28	6854.0
29	8234.5
30	9615.0
31	11302.5
32	12990.0
33	14796.0
34	16602.0
35	18924.0
36	21246.0
37	24246.5
38	27247.0
39	29798.0
40	32349.0
41	35087.5
42	37826.0
43	39915.0
44	42004.0
45	44794.5
46	47585.0
47	49573.5
48	51562.0
49	53252.0
50	54942.0
51	55460.0
52	55978.0
53	56027.5
54	56077.0
55	54494.0
56	52911.0
57	50340.5
58	47770.0
59	44031.0
60	40292.0
61	35576.0
62	30860.0
63	26447.5
64	22035.0
65	18455.0
66	14875.0
67	12621.5
68	10368.0
69	8579.5
70	6791.0
71	5465.5
72	4140.0
73	3479.5
74	2819.0
75	2202.5
76	1586.0
77	1302.5
78	1019.0
79	862.5
80	706.0
81	527.0
82	348.0
83	256.0
84	164.0
85	125.0
86	86.0
87	57.5
88	29.0
89	20.0
90	11.0
91	11.5
92	12.0
93	7.5
94	3.0
95	4.5
96	6.0
97	4.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03198880118549951
2	0.0047846497499678745
3	2.734085571410214E-4
4	1.367042785705107E-4
5	1.367042785705107E-4
6	1.367042785705107E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.468171142820428E-4
17	0.0
18	1.367042785705107E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	1.367042785705107E-4
24	6.835213928525535E-4
25	4.1011283571153206E-4
26	1.367042785705107E-4
27	4.1011283571153206E-4
28	0.0016404513428461282
29	0.0
30	8.202256714230641E-4
31	5.468171142820428E-4
32	4.1011283571153206E-4
33	0.0
34	5.468171142820428E-4
35	1.367042785705107E-4
36	2.734085571410214E-4
37	0.001367042785705107
38	0.0
39	8.202256714230641E-4
40	1.367042785705107E-4
41	4.1011283571153206E-4
42	0.004647945471397364
43	0.002870789849980725
44	0.004101128357115321
45	0.001367042785705107
46	0.0021872684571281713
47	0.0012303385071345963
48	0.0043745369142563426
49	0.0038277197999742997
50	0.0016404513428461282
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	731506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.08351223580368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25789527850205	48.86873765156521
2	14.323225349090048	18.357651738291715
3	4.498512153664384	8.648413759267894
4	1.7873231237314313	4.5815177307591215
5	0.938904333592952	3.008414367502652
6	0.5452387699792388	2.096448923243946
7	0.3574330620254379	1.6033896202651547
8	0.2581028018460694	1.323210724815824
9	0.18429975734187443	1.0629518179206294
>10	0.8177598827915935	8.705845115494881
>50	0.02594514000282221	1.0819559073163534
>100	0.005145933535570144	0.5395853349492625
>500	2.1441389648452008E-4	0.12187730860737281
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	887	0.12125669509204298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.367042785705107E-4
7	0.0	0.0	0.0	0.0	1.367042785705107E-4
8	0.0	0.0	0.0	1.367042785705107E-4	1.367042785705107E-4
9	0.0	0.0	0.0	1.367042785705107E-4	1.367042785705107E-4
10	0.0	0.0	0.0	2.734085571410214E-4	1.367042785705107E-4
11	0.0	0.0	0.0	4.101128357115321E-4	1.367042785705107E-4
12	0.0	0.0	0.0	4.101128357115321E-4	6.835213928525535E-4
13	0.0	0.0	0.0	4.101128357115321E-4	6.835213928525535E-4
14	0.0	0.0	0.0	5.468171142820428E-4	6.835213928525535E-4
15	0.0	0.0	0.0	9.569299499935749E-4	6.835213928525535E-4
16	0.0	0.0	0.0	0.0010936342285640856	8.202256714230642E-4
17	0.0	0.0	0.0	0.0012303385071345963	8.202256714230642E-4
18	0.0	0.0	0.0	0.001367042785705107	9.569299499935749E-4
19	0.0	0.0	0.0	0.0015037470642756178	9.569299499935749E-4
20	0.0	0.0	0.0	0.0023239727356986818	0.0012303385071345963
21	0.0	0.0	0.0	0.0028707898499807246	0.001367042785705107
22	0.0	0.0	0.0	0.003691015521403789	0.001367042785705107
23	0.0	0.0	0.0	0.005468171142820428	0.001367042785705107
24	0.0	0.0	0.0	0.006425101092814003	0.001367042785705107
25	0.0	0.0	0.0	0.008475665271371663	0.001367042785705107
26	0.0	0.0	0.0	0.010799638007070345	0.001367042785705107
27	0.0	0.0	0.0	0.012576793628486984	0.001367042785705107
28	0.0	0.0	0.0	0.016131104871320263	0.001367042785705107
29	0.0	0.0	0.0	0.02146257173557018	0.001367042785705107
30	0.0	0.0	0.0	0.03144198407121746	0.001367042785705107
31	0.0	0.0	0.0	0.04962365312109539	0.001367042785705107
32	0.0	0.0	0.0	0.06548134943527463	0.001367042785705107
33	0.0	0.0	0.0	0.08065552435660131	0.001367042785705107
34	0.0	0.0	0.0	0.09815367201362668	0.001367042785705107
35	0.0	0.0	0.0	0.1163353410635046	0.001367042785705107
36	0.0	0.0	0.0	0.14476983100617083	0.001367042785705107
37	0.0	0.0	0.0	0.18058635199164463	0.001367042785705107
38	0.0	0.0	0.0	0.23007330083416952	0.001367042785705107
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAGA	50	0.0025782406	22.00181	37
ATAGGGG	50	0.0025802883	21.998802	3
TTCGTAT	55	0.0044815848	20.00028	25
CCGCTAG	55	0.0044815848	20.00028	36
ATTGCGC	55	0.004483358	19.998913	30
GTATCAA	1420	0.0	19.05921	1
AGGCGTT	60	0.007402935	18.336096	42
GCTTAAC	60	0.007402935	18.336096	1
ATAATAC	60	0.007411698	18.332336	3
TAACGGA	60	0.007411698	18.332336	4
TATTGCG	60	0.007411698	18.332336	29
ACTTATC	85	1.4304499E-4	18.116663	8
GTCCTAT	110	2.7893439E-6	18.002714	1
AAATCCG	75	0.0012906801	17.599043	29
ACGCTCC	75	0.0012906801	17.599043	35
GTTATAG	90	2.2113182E-4	17.113691	1
TAAGACT	155	1.4373654E-8	17.031332	4
CTATAAC	105	3.7728154E-5	16.760994	3
ATTCGTA	80	0.0019862463	16.501358	24
GCATTCG	80	0.0019862463	16.501358	22
>>END_MODULE