FastQCFastQC Report
Thu 2 Feb 2017
SRR4062124_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062124_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201212
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14370.7141721169711548No Hit
TATCAACGCAGAGTACTTTTTTTTT10100.5019581337097191No Hit
GGTATCAACGCAGAGTACTTTTTTT5620.27930739717313086No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5490.2728465499075602No Hit
CCATAGGGTCTTCTCGTCTTATTAT4640.23060254855575216No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3430.1704669701608254No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3250.16152118163926607No Hit
GTACATGGGGTGGTATCAACGCAAA2800.13915671033536767No Hit
GTATCAACGCAGAGTACATGGGGTG2690.1336898395721925No Hit
CTTCTACACCATTGGGATGTCCTGA2490.12375007454823769No Hit
CCATTGGGATGTCCTGATCCAACAT2440.12126513329224896No Hit
GTATTAGAGGCACTGCCTGCCCAGT2420.12027115678985348No Hit
ATCCTGACCGTGCAAAGGTAGCATA2390.11878019203626025No Hit
ATAAATAATCCACCTATAACTTCTC2340.11629525078027156No Hit
GTCAGGATACCGCGGCCGTTAAACT2300.11430729777548058No Hit
GTTCATGCTAGTCCCTAATTAAGGA2180.10834343876110768No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2080.10337355624913028No Hit
CCTATAACTTCTCTGTTAACCCAAC2080.10337355624913028No Hit
GGTCAGGATACCGCGGCCGTTAAAC2070.10287656799793253No Hit
ATCGTAAATAGATAGAAACCGACCT2050.10188259149553705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACAAG250.00601921418.99726118
TATATGG250.006027881518.992532
GACAGTG350.002159845516.2874227
TAAGTTG350.002159845516.2874225
TGCAAAT350.002159845516.28742210
GTGCTGG350.00217065616.275261
TCTAAGG456.733482E-414.7756473
CAGAACA400.005254285414.2514944
CGTGCCG400.005254285414.25149411
GCAAATC400.005254285414.25149411
TGACGTG400.005254285414.2514945
TGAGTAC400.005254285414.2514945
CTCGGCA400.005254285414.25149412
CTTGCCT400.005254285414.2514944
CAGCTAG400.005254285414.2514949
GCGCTTA400.005254285414.25149413
ACCGGCG400.0052629814.24794619
CTCTAAG400.005271686214.2443992
GTTATAT551.9574491E-413.8093131
CTTATTA707.198236E-613.56947218
Produced by FastQC (version 0.11.5)