##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062124_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201212
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.002678766673956	32.0	32.0	32.0	32.0	32.0
2	30.65263503170785	32.0	32.0	32.0	32.0	32.0
3	30.726084925352364	32.0	32.0	32.0	32.0	32.0
4	30.6864302327893	32.0	32.0	32.0	32.0	32.0
5	30.64931018030734	32.0	32.0	32.0	32.0	32.0
6	34.22284953183707	36.0	36.0	36.0	32.0	36.0
7	34.13501679820289	36.0	36.0	36.0	32.0	36.0
8	34.000039759060094	36.0	36.0	36.0	32.0	36.0
9	34.10859690276922	36.0	36.0	36.0	32.0	36.0
10	33.855575214201934	36.0	36.0	36.0	32.0	36.0
11	34.23144742858279	36.0	36.0	36.0	32.0	36.0
12	34.03461523169592	36.0	36.0	36.0	32.0	36.0
13	34.15621334711648	36.0	36.0	36.0	32.0	36.0
14	34.01706657654613	36.0	36.0	36.0	32.0	36.0
15	33.99649623282905	36.0	36.0	36.0	32.0	36.0
16	33.98154185635052	36.0	36.0	36.0	32.0	36.0
17	33.89096574756973	36.0	36.0	36.0	32.0	36.0
18	33.952612170248294	36.0	36.0	36.0	32.0	36.0
19	33.88427628570861	36.0	36.0	36.0	32.0	36.0
20	33.83154583225652	36.0	36.0	36.0	32.0	36.0
21	33.83318092360297	36.0	36.0	36.0	32.0	36.0
22	33.830611494344275	36.0	36.0	36.0	32.0	36.0
23	33.820646879907756	36.0	36.0	36.0	32.0	36.0
24	33.79510665367871	36.0	36.0	36.0	32.0	36.0
25	33.52191221199531	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	14.0
6	41.0
7	12.0
8	37.0
9	18.0
10	16.0
11	3.0
12	13.0
13	8.0
14	63.0
15	80.0
16	122.0
17	211.0
18	241.0
19	344.0
20	481.0
21	625.0
22	839.0
23	1085.0
24	1370.0
25	1829.0
26	2412.0
27	3214.0
28	4003.0
29	5531.0
30	7300.0
31	10132.0
32	14572.0
33	20316.0
34	43766.0
35	82510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21384019760351	19.918891114302895	12.498819647037191	24.368449041056405
2	16.951275271852573	19.718008866270402	37.840685446196055	25.490030415680977
3	18.603264218833868	24.017454227382064	29.085243424845437	28.29403812893863
4	12.569338131498897	16.067558701314194	35.86148279221425	35.50162037497267
5	13.847155797281577	38.066379644636235	32.28202400246587	15.80444055561632
6	31.203843227356142	37.49285113959051	16.41477812423849	14.888527508814855
7	29.34070963761915	32.74263198742314	18.636445045869735	19.280213329087978
8	28.090793296978866	32.464275763244835	17.975062691557536	21.469868248218763
9	26.60320840714129	15.218537905777918	20.023684891427664	38.15456879565313
10	17.33277596134227	25.940191994744776	28.740488596268595	27.986543447644358
11	35.149566801196336	23.28176086231693	21.193648076358443	20.375024260128292
12	24.203428628299868	25.371351795178025	27.10457565126521	23.3206439252569
13	27.33759964967107	20.741562745702996	26.367228320909263	25.55360928371667
14	22.835307756223038	21.123754272664403	25.207600491574084	30.833337479538475
15	26.040915039835184	26.653496091125785	22.97900504097973	24.3265838280593
16	24.926109845054583	27.80260133151552	22.41085911610457	24.860429707325327
17	22.042424875725867	26.997964839998605	25.241457552732538	25.718152731542986
18	22.641584532794674	26.33325538700353	26.38400342300478	24.641156657197016
19	25.283176551936844	25.85972749386886	26.43229879168055	22.424797162513745
20	24.762477988797915	25.13853378035556	26.158261786563468	23.940726444283055
21	26.049086712563174	23.036133550877473	26.975804846989533	23.93897488956982
22	24.97873335356359	24.769300414388688	26.073654729154956	24.17831150289276
23	24.402532615129044	24.888464465300885	26.621803767090924	24.087199152479148
24	23.872103781374665	26.318407465255323	25.853818681045375	23.955670072324637
25	24.06249533672571	25.694019568342462	26.360059490944543	23.883425603987284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	133.0
1	133.0
2	163.5
3	194.0
4	194.0
5	194.0
6	462.5
7	731.0
8	731.0
9	731.0
10	724.0
11	717.0
12	717.0
13	717.0
14	747.5
15	778.0
16	778.0
17	778.0
18	1260.5
19	1743.0
20	1743.0
21	1743.0
22	2809.0
23	3875.0
24	3875.0
25	3875.0
26	5963.5
27	8052.0
28	8052.0
29	8052.0
30	9192.5
31	10333.0
32	10333.0
33	10333.0
34	11816.0
35	13299.0
36	13299.0
37	13299.0
38	14123.0
39	14947.0
40	14947.0
41	14947.0
42	16699.0
43	18451.0
44	18451.0
45	18451.0
46	22332.5
47	26214.0
48	26214.0
49	26214.0
50	27138.0
51	28062.0
52	28062.0
53	28062.0
54	25857.0
55	23652.0
56	23652.0
57	23652.0
58	21022.0
59	18392.0
60	18392.0
61	18392.0
62	15727.0
63	13062.0
64	13062.0
65	13062.0
66	10900.0
67	8738.0
68	8738.0
69	8738.0
70	6689.5
71	4641.0
72	4641.0
73	4641.0
74	3829.5
75	3018.0
76	3018.0
77	3018.0
78	2167.0
79	1316.0
80	1316.0
81	1316.0
82	894.5
83	473.0
84	473.0
85	473.0
86	358.5
87	244.0
88	244.0
89	244.0
90	148.5
91	53.0
92	53.0
93	53.0
94	34.0
95	15.0
96	15.0
97	15.0
98	47.0
99	79.0
100	79.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.969882511977417E-4
2	0.0
3	0.0
4	0.011927718028745801
5	0.03280122457905095
6	0.06510546090690417
7	0.10337355624913028
8	0.1133133212730851
9	0.119277180287458
10	0.1336898395721925
11	0.13170188656740153
12	0.1277259805578196
13	0.12822296880901735
14	0.11182235651949189
15	0.1287199570602151
16	0.11828320378506252
17	0.1217621215434467
18	0.10884042701230542
19	0.09393077947637318
20	0.08846390871319802
21	0.0894578852155935
22	0.09591873248116414
23	0.07703317893564997
24	0.08647595570840705
25	0.0859789674572093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	201212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.54471900284277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.36505867014341	20.24928930680079
2	15.36387341472087	10.307536329841163
3	7.455256607798981	7.502534640081109
4	4.421002726087472	5.932051766296245
5	2.860910276164513	4.798421565314196
6	1.9838212634822803	3.9928036101226567
7	1.3600805973687329	3.1936465021966884
8	0.9822804314329738	2.6360256843528216
9	0.734858361977006	2.218555553346719
>10	4.066907668602584	24.574578056974733
>50	0.28890600924499227	6.5667057630757615
>100	0.11111769586345857	6.259567023835556
>500	0.0029631385563588953	0.552153947080691
>1k	0.0029631385563588953	1.216130250680874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1437	0.7141721169711548	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1010	0.5019581337097191	No Hit
GGTATCAACGCAGAGTACTTTTTTT	562	0.27930739717313086	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	549	0.2728465499075602	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	464	0.23060254855575216	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	343	0.1704669701608254	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	325	0.16152118163926607	No Hit
GTACATGGGGTGGTATCAACGCAAA	280	0.13915671033536767	No Hit
GTATCAACGCAGAGTACATGGGGTG	269	0.1336898395721925	No Hit
CTTCTACACCATTGGGATGTCCTGA	249	0.12375007454823769	No Hit
CCATTGGGATGTCCTGATCCAACAT	244	0.12126513329224896	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	242	0.12027115678985348	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	239	0.11878019203626025	No Hit
ATAAATAATCCACCTATAACTTCTC	234	0.11629525078027156	No Hit
GTCAGGATACCGCGGCCGTTAAACT	230	0.11430729777548058	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	218	0.10834343876110768	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	208	0.10337355624913028	No Hit
CCTATAACTTCTCTGTTAACCCAAC	208	0.10337355624913028	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	207	0.10287656799793253	No Hit
ATCGTAAATAGATAGAAACCGACCT	205	0.10188259149553705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACAAG	25	0.006019214	18.997261	18
TATATGG	25	0.0060278815	18.99253	2
GACAGTG	35	0.0021598455	16.287422	7
TAAGTTG	35	0.0021598455	16.287422	5
TGCAAAT	35	0.0021598455	16.287422	10
GTGCTGG	35	0.002170656	16.27526	1
TCTAAGG	45	6.733482E-4	14.775647	3
CAGAACA	40	0.0052542854	14.251494	4
CGTGCCG	40	0.0052542854	14.251494	11
GCAAATC	40	0.0052542854	14.251494	11
TGACGTG	40	0.0052542854	14.251494	5
TGAGTAC	40	0.0052542854	14.251494	5
CTCGGCA	40	0.0052542854	14.251494	12
CTTGCCT	40	0.0052542854	14.251494	4
CAGCTAG	40	0.0052542854	14.251494	9
GCGCTTA	40	0.0052542854	14.251494	13
ACCGGCG	40	0.00526298	14.247946	19
CTCTAAG	40	0.0052716862	14.244399	2
GTTATAT	55	1.9574491E-4	13.809313	1
CTTATTA	70	7.198236E-6	13.569472	18
>>END_MODULE