FastQCFastQC Report
Thu 2 Feb 2017
SRR4062124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201212
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG4890.24302725483569568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.178915770431187No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT2610.12971393356261057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.12623501580422639No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA2390.11878019203626025No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA2330.11579826252907381No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2200.10933741526350316No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2120.10536150925392124No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT2120.10536150925392124No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2070.10287656799793253No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC2060.10237957974673478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGT207.842515E-443.9982622
GGTATTT301.2975841E-436.6652230
GGCAACC250.002348604835.1986129
TGCGCTA250.002348604835.1986110
GCTAGTG250.002348604835.1986125
GTGAGAT250.002348604835.1986127
AACAGGA250.002348604835.1986133
ACGTTAG401.8130704E-532.9986951
ATACATG551.3645513E-731.9987341
GAACAGG353.209154E-431.4273332
ACCAGAA300.005720969329.3467642
GAGTTAA300.00572792729.33946641
TTTAGAG300.005734891629.3321762
CCTAGTG300.005734891629.3321763
CGTGAGA300.005734891629.33217626
ATCAGCA300.005734891629.33217634
ACTAGAC300.005734891629.3321763
CTAGTGT300.005734891629.3321764
CTGCAAT300.005734891629.33217611
TCCGTCG300.005734891629.33217633
Produced by FastQC (version 0.11.5)