##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201212
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.530480289445958	32.0	32.0	32.0	32.0	32.0
2	31.25180406735185	32.0	32.0	32.0	32.0	32.0
3	31.447711866091485	32.0	32.0	32.0	32.0	32.0
4	31.59133153092261	32.0	32.0	32.0	32.0	32.0
5	31.488688547402738	32.0	32.0	32.0	32.0	32.0
6	35.15044331352007	36.0	36.0	36.0	36.0	36.0
7	35.16299226686281	36.0	36.0	36.0	36.0	36.0
8	35.110092837405325	36.0	36.0	36.0	36.0	36.0
9	35.20696081744627	36.0	36.0	36.0	36.0	36.0
10	35.04801403494822	36.0	36.0	36.0	36.0	36.0
11	35.24256505576208	36.0	36.0	36.0	36.0	36.0
12	35.12626980498181	36.0	36.0	36.0	36.0	36.0
13	35.19674770888416	36.0	36.0	36.0	36.0	36.0
14	35.1125728087788	36.0	36.0	36.0	36.0	36.0
15	35.10948154185635	36.0	36.0	36.0	36.0	36.0
16	35.13376935769239	36.0	36.0	36.0	36.0	36.0
17	35.106693437767134	36.0	36.0	36.0	36.0	36.0
18	35.10747370932151	36.0	36.0	36.0	36.0	36.0
19	35.07147685028726	36.0	36.0	36.0	36.0	36.0
20	35.04156809733018	36.0	36.0	36.0	36.0	36.0
21	35.03169791066139	36.0	36.0	36.0	36.0	36.0
22	35.02619128083812	36.0	36.0	36.0	36.0	36.0
23	35.03742321531519	36.0	36.0	36.0	36.0	36.0
24	35.01420392421923	36.0	36.0	36.0	36.0	36.0
25	35.016683895592706	36.0	36.0	36.0	36.0	36.0
26	34.96213446514125	36.0	36.0	36.0	36.0	36.0
27	34.95715961274676	36.0	36.0	36.0	36.0	36.0
28	34.93370176729022	36.0	36.0	36.0	32.0	36.0
29	34.91678428721945	36.0	36.0	36.0	32.0	36.0
30	34.90366876727034	36.0	36.0	36.0	32.0	36.0
31	34.89377373118899	36.0	36.0	36.0	32.0	36.0
32	34.85575412997237	36.0	36.0	36.0	32.0	36.0
33	34.80597081684989	36.0	36.0	36.0	32.0	36.0
34	34.80143331411645	36.0	36.0	36.0	32.0	36.0
35	34.78324354412262	36.0	36.0	36.0	32.0	36.0
36	34.79129475379202	36.0	36.0	36.0	32.0	36.0
37	34.75691807645667	36.0	36.0	36.0	32.0	36.0
38	34.74550722620917	36.0	36.0	36.0	32.0	36.0
39	34.70340735145021	36.0	36.0	36.0	32.0	36.0
40	34.67839393276743	36.0	36.0	36.0	32.0	36.0
41	34.60700654036538	36.0	36.0	36.0	32.0	36.0
42	34.58385185774208	36.0	36.0	36.0	32.0	36.0
43	34.52872095103672	36.0	36.0	36.0	32.0	36.0
44	34.44882511977417	36.0	36.0	36.0	32.0	36.0
45	34.387158817565556	36.0	36.0	36.0	32.0	36.0
46	34.32205335665865	36.0	36.0	36.0	32.0	36.0
47	34.230786434208696	36.0	36.0	36.0	32.0	36.0
48	34.15697373914081	36.0	36.0	36.0	32.0	36.0
49	34.12140429000259	36.0	36.0	36.0	32.0	36.0
50	33.703591237103154	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	5.0
22	22.0
23	56.0
24	115.0
25	280.0
26	570.0
27	1104.0
28	1840.0
29	2902.0
30	4519.0
31	6737.0
32	10339.0
33	17013.0
34	37009.0
35	118697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94183266140057	21.429104050984037	13.054220251440388	24.574843036175004
2	16.32248348666259	20.318487482666587	37.271683540340256	26.087345490330566
3	18.364212870007755	24.07957775878178	29.38542432856887	28.170785042641594
4	12.496272588116017	16.3946484305111	35.839810746873944	35.26926823449894
5	13.583185893485478	37.98232709778741	32.36834781225772	16.066139196469393
6	31.209868198715785	37.75570045524124	16.286801980001194	14.747629366041787
7	29.313361032145203	32.66753473947876	18.8159751903465	19.20312903802954
8	28.016718684770293	32.46675148599487	17.904498737649842	21.612031091584996
9	26.63459435818937	14.771981790350477	20.112120549470212	38.48130330198994
10	17.37222432061706	25.699262469435226	28.796493250899545	28.132019959048165
11	35.681768482993064	22.914637297974274	21.30290439934	20.10068981969266
12	23.95731864898714	25.653539550325032	27.3104983798183	23.07864342086953
13	27.567441305687534	20.542512375007455	26.708645607617836	25.181400711687175
14	22.927558992505418	20.852633043754846	25.26688269089319	30.95292527284655
15	26.236009780728786	26.510844283641134	22.85400473132815	24.39914120430193
16	25.274588738134284	27.322200685850607	22.688236171164455	24.714974404850654
17	22.292904995725902	26.566009979524086	25.267379679144387	25.87370534560563
18	22.657806978743707	25.974723051920623	26.71523922648364	24.65223074285203
19	25.291235115201875	25.516370792994454	26.865693894996323	22.326700196807348
20	24.978132516947298	24.681430530982247	26.33540743096833	24.005029521102124
21	25.99745542015387	23.14225791702284	27.032681947398764	23.827604715424528
22	24.780828181221796	24.44784605291931	26.479037035564478	24.292288730294416
23	24.367333956225277	24.316641154603104	27.08387173727213	24.23215315189949
24	23.83853845695088	26.011371091187403	26.039202433254477	24.11088801860724
25	24.354533300863274	25.277942060821722	26.708778347108257	23.658746291206743
26	22.863944496352108	25.114804286026676	27.684730532970203	24.336520684651013
27	23.783372761067927	26.15947359004433	26.379142397073736	23.678011251814006
28	23.637990159534812	25.042492917847024	26.872421847820682	24.447095074797474
29	23.706457400440335	25.927011942687027	26.399650118532286	23.966880538340348
30	23.813925749217233	25.38740619253516	27.25460961184832	23.544058446399283
31	23.523681725560362	24.88693404900353	26.812782664877492	24.77660156055862
32	23.14275490527404	25.29521102121146	26.66093473550285	24.90109933801165
33	23.766475160527207	25.77331371886369	26.458163529014172	24.002047591594934
34	23.69365339694846	24.745787982704638	27.3445653794543	24.2159932408926
35	23.911098741625747	25.145617557600943	26.26483509929825	24.67844860147506
36	23.74709261873049	24.898117408504465	26.52128103691629	24.833508935848755
37	24.581902399992046	25.32391692220527	25.371131510021915	24.723049167780765
38	24.15909587897342	25.159036240383276	26.642546170208536	24.039321710434766
39	23.775042119963622	25.58756728011888	26.170040405345635	24.467350194571868
40	23.416595431683994	26.18332902610182	26.731010078921734	23.669065463292448
41	23.613402779158303	26.20370554440093	26.911913802357713	23.27097787408306
42	23.269336858689226	26.38649364591047	25.868127171249792	24.47604232415051
43	23.66728626382911	26.554377106050513	25.386916891146388	24.391419738973987
44	23.758498668045007	25.50693809391277	26.425390640531194	24.309172597511033
45	24.32656753210608	25.995984255099202	25.849369806369527	23.828078406425192
46	25.362066737572437	26.235798137232486	24.691609594147295	23.710525531047782
47	24.611469779878433	26.518958087939286	24.523997674037183	24.345574458145094
48	24.49143907954574	27.154891777043318	24.030217936929997	24.323451206480954
49	24.13421204349814	26.37919723265939	25.061628993459372	24.424961730383092
50	23.21030972923542	26.844360065206157	25.234583117967475	24.71074708759095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	45.0
2	23.0
3	48.0
4	73.0
5	74.0
6	75.0
7	57.5
8	40.0
9	69.0
10	98.0
11	173.0
12	248.0
13	457.5
14	667.0
15	970.0
16	1273.0
17	1436.5
18	1600.0
19	1708.5
20	1817.0
21	1927.0
22	2037.0
23	1754.0
24	1471.0
25	1801.5
26	2132.0
27	2593.5
28	3055.0
29	3387.0
30	3719.0
31	3677.5
32	3636.0
33	3858.5
34	4081.0
35	4745.5
36	5410.0
37	6679.0
38	7948.0
39	8352.0
40	8756.0
41	9977.0
42	11198.0
43	10723.0
44	10248.0
45	10815.5
46	11383.0
47	11978.0
48	12573.0
49	13434.0
50	14295.0
51	14879.0
52	15463.0
53	14796.5
54	14130.0
55	13901.5
56	13673.0
57	12874.5
58	12076.0
59	10950.0
60	9824.0
61	8822.5
62	7821.0
63	6472.5
64	5124.0
65	4733.5
66	4343.0
67	3894.5
68	3446.0
69	2964.0
70	2482.0
71	2159.0
72	1836.0
73	1577.5
74	1319.0
75	991.5
76	664.0
77	544.5
78	425.0
79	366.5
80	308.0
81	232.5
82	157.0
83	137.5
84	118.0
85	84.5
86	51.0
87	29.0
88	7.0
89	6.5
90	6.0
91	7.5
92	9.0
93	6.0
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025346400811084826
2	0.005466870763175159
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.939765023954834E-4
17	0.0
18	4.969882511977417E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	4.969882511977417E-4
26	0.0
27	0.0
28	9.939765023954834E-4
29	4.969882511977417E-4
30	9.939765023954834E-4
31	9.939765023954834E-4
32	0.0
33	0.0
34	9.939765023954834E-4
35	0.0
36	0.0
37	0.0014909647535932252
38	0.0
39	4.969882511977417E-4
40	0.0
41	0.0
42	0.0014909647535932252
43	0.0029819295071864503
44	0.001987953004790967
45	0.001987953004790967
46	0.0029819295071864503
47	0.002484941255988708
48	0.003478917758384192
49	0.003975906009581934
50	0.001987953004790967
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	201212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.783651074488596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.69082373855919	21.717392600838917
2	15.202566631019012	10.88006679522096
3	7.631838446688241	8.192851320994771
4	4.373550367356009	6.260064012086754
5	2.9457924195497287	5.270560403952051
6	1.9083068290718184	4.097171142874183
7	1.3319259454729795	3.3362821302904395
8	1.02498576408661	2.934218635071467
9	0.8083221066374078	2.603224459773771
>10	3.7624477437813364	23.99459276782697
>50	0.2319412230385689	5.584656978709023
>100	0.08749878473910085	5.128918752360694
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	489	0.24302725483569568	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.178915770431187	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	261	0.12971393356261057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.12623501580422639	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	239	0.11878019203626025	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	233	0.11579826252907381	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	220	0.10933741526350316	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	212	0.10536150925392124	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	212	0.10536150925392124	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	207	0.10287656799793253	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	206	0.10237957974673478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	4.969882511977417E-4	0.0
19	0.0	0.0	0.0	4.969882511977417E-4	0.0
20	0.0	0.0	0.0	4.969882511977417E-4	0.0
21	0.0	0.0	0.0	4.969882511977417E-4	0.0
22	0.0	0.0	0.0	9.939765023954834E-4	0.0
23	0.0	0.0	0.0	0.001987953004790967	0.0
24	0.0	0.0	0.0	0.003975906009581934	0.0
25	0.0	0.0	0.0	0.005466870763175159	0.0
26	0.0	0.0	0.0	0.005466870763175159	0.0
27	0.0	0.0	0.0	0.005963859014372901	0.0
28	0.0	0.0	0.0	0.007951812019163867	0.0
29	0.0	0.0	0.0	0.010933741526350318	0.0
30	0.0	0.0	0.0	0.023855436057491602	0.0
31	0.0	0.0	0.0	0.03975906009581934	0.0
32	0.0	0.0	0.0	0.060135578394926746	0.0
33	0.0	0.0	0.0	0.07206329642367254	0.0
34	0.0	0.0	0.0	0.09243981472277996	0.0
35	0.0	0.0	0.0	0.11679223903146929	0.0
36	0.0	0.0	0.0	0.14859948710812476	0.0
37	0.0	0.0	0.0	0.18289167644076895	0.0
38	0.0	0.0	0.0	0.22960857205335666	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGT	20	7.842515E-4	43.99826	22
GGTATTT	30	1.2975841E-4	36.66522	30
GGCAACC	25	0.0023486048	35.19861	29
TGCGCTA	25	0.0023486048	35.19861	10
GCTAGTG	25	0.0023486048	35.19861	25
GTGAGAT	25	0.0023486048	35.19861	27
AACAGGA	25	0.0023486048	35.19861	33
ACGTTAG	40	1.8130704E-5	32.998695	1
ATACATG	55	1.3645513E-7	31.998734	1
GAACAGG	35	3.209154E-4	31.42733	32
ACCAGAA	30	0.0057209693	29.34676	42
GAGTTAA	30	0.005727927	29.339466	41
TTTAGAG	30	0.0057348916	29.332176	2
CCTAGTG	30	0.0057348916	29.332176	3
CGTGAGA	30	0.0057348916	29.332176	26
ATCAGCA	30	0.0057348916	29.332176	34
ACTAGAC	30	0.0057348916	29.332176	3
CTAGTGT	30	0.0057348916	29.332176	4
CTGCAAT	30	0.0057348916	29.332176	11
TCCGTCG	30	0.0057348916	29.332176	33
>>END_MODULE