FastQCFastQC Report
Thu 2 Feb 2017
SRR4062123_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062123_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407248
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42320.17580241005496733No Hit
TATCAACGCAGAGTACTTTTTTTTT27470.1141137099293467No Hit
GCGCAAGACGGACCAGAGCGAAAGC24400.10136055778216452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA2150.014.1421079
GGTATCA10500.013.7454411
GCGTTAT3450.013.2107221
CGAACGA1102.752131E-912.95339616
CGCGTAA2500.012.54254110
GTATCAA26800.012.2233071
CGGTCCA4150.012.13498910
GGGTATA550.003080908412.0848071
GTATAGA1850.011.8048521
CGTTATT3550.011.7718892
GCGTAAC2600.011.69467711
ACTCTAA3750.011.4023110
CGCATCG3650.011.19311713
CGCGCTA855.3302494E-511.17547916
CGCATGT600.00586929511.08534912
CGAGCCG4650.011.03219815
GGTCGCG3050.010.9036217
TAACGAA1408.341885E-910.858438513
AGAACCG2450.010.8582135
CGCCAGT3950.010.82092518
Produced by FastQC (version 0.11.5)