##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062123_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2407248
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29217596192831	32.0	32.0	32.0	32.0	32.0
2	30.875074358769847	32.0	32.0	32.0	32.0	32.0
3	30.903532166191436	32.0	32.0	32.0	32.0	32.0
4	30.905946541444838	32.0	32.0	32.0	32.0	32.0
5	30.83542161007092	32.0	32.0	32.0	32.0	32.0
6	34.46827871494752	36.0	36.0	36.0	32.0	36.0
7	34.4216711365011	36.0	36.0	36.0	32.0	36.0
8	34.383599861750845	36.0	36.0	36.0	32.0	36.0
9	34.50609824995181	36.0	36.0	36.0	32.0	36.0
10	34.22652173768552	36.0	36.0	36.0	32.0	36.0
11	34.475944314835864	36.0	36.0	36.0	32.0	36.0
12	34.324878865825205	36.0	36.0	36.0	32.0	36.0
13	34.403683376203865	36.0	36.0	36.0	32.0	36.0
14	34.31575641562481	36.0	36.0	36.0	32.0	36.0
15	34.273820146490934	36.0	36.0	36.0	32.0	36.0
16	34.27514904986939	36.0	36.0	36.0	32.0	36.0
17	34.21102063435092	36.0	36.0	36.0	32.0	36.0
18	34.21580452034855	36.0	36.0	36.0	32.0	36.0
19	34.21534507454155	36.0	36.0	36.0	32.0	36.0
20	34.20149191109516	36.0	36.0	36.0	32.0	36.0
21	34.1907410453763	36.0	36.0	36.0	32.0	36.0
22	34.16525800416077	36.0	36.0	36.0	32.0	36.0
23	34.133987856672846	36.0	36.0	36.0	32.0	36.0
24	34.102485078396576	36.0	36.0	36.0	32.0	36.0
25	33.76147035951427	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	23.0
5	185.0
6	531.0
7	105.0
8	462.0
9	327.0
10	190.0
11	47.0
12	99.0
13	100.0
14	328.0
15	497.0
16	884.0
17	1090.0
18	1507.0
19	2045.0
20	2975.0
21	4196.0
22	6188.0
23	8663.0
24	12568.0
25	18148.0
26	25233.0
27	32746.0
28	44359.0
29	58761.0
30	78647.0
31	108189.0
32	155983.0
33	224337.0
34	503063.0
35	1114771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.04577894833044	17.34660728973671	11.838136938044716	25.769476823888137
2	16.98126610505554	19.128246818660866	36.87204563232348	27.018441443960118
3	19.053870036946908	22.652932530975335	28.13913705250582	30.15406037957194
4	13.15578795093828	15.307587134073867	34.592558648513574	36.94406626647429
5	15.448668375151314	35.91225309287112	32.86245424221281	15.77662428976476
6	35.490697502346514	34.27595649994305	16.16595266737554	14.067393330334896
7	31.1315505370513	29.95664165362229	19.8337611223765	19.07804668694991
8	28.661215411896613	32.56409424665556	18.745446197762273	20.02924414368556
9	27.045436113069353	14.485903762531896	18.400825165896194	40.06783495850256
10	16.82124340261832	26.26365233712038	30.35111714553841	26.56398711472289
11	37.274888988915116	21.23234160380129	21.645102324888697	19.847667082394892
12	24.67801661060746	23.380349300292927	27.997933449417346	23.943700639682262
13	29.336024281760935	19.237064542414675	24.98143802964009	26.445473146184305
14	23.849944893842668	19.288153215912164	24.455114475243818	32.406787415001354
15	25.43078418028709	26.775674421003764	21.646572279062955	26.14696911964619
16	26.200027367166307	25.672772705701895	22.96117730388493	25.16602262324687
17	24.4840721200959	25.905743753046735	24.45333439813128	25.156849728726087
18	25.165023089924276	24.97155347945862	25.29627633382574	24.567147096791366
19	25.8077608077421	24.991776955265387	24.811514508112225	24.38894772888029
20	25.99949770432581	24.199757334639525	24.37535291676611	25.42539204426855
21	26.80320121881496	24.097624949115556	24.195755520156474	24.903418311913008
22	26.2384756455094	23.90886143328246	24.657856106192483	25.194806815015657
23	24.82100605800065	24.108397924705507	25.12754396738134	25.943052049912502
24	25.055799858961908	24.670938834706018	24.824367657037936	25.448893649294146
25	25.13510063578482	24.296659666145402	24.707834610294384	25.860405087775394
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	695.0
1	695.0
2	705.0
3	715.0
4	715.0
5	715.0
6	1538.5
7	2362.0
8	2362.0
9	2362.0
10	2875.0
11	3388.0
12	3388.0
13	3388.0
14	4493.0
15	5598.0
16	5598.0
17	5598.0
18	9787.5
19	13977.0
20	13977.0
21	13977.0
22	21482.5
23	28988.0
24	28988.0
25	28988.0
26	43120.0
27	57252.0
28	57252.0
29	57252.0
30	71768.5
31	86285.0
32	86285.0
33	86285.0
34	110744.5
35	135204.0
36	135204.0
37	135204.0
38	161157.0
39	187110.0
40	187110.0
41	187110.0
42	217536.0
43	247962.0
44	247962.0
45	247962.0
46	276872.5
47	305783.0
48	305783.0
49	305783.0
50	319623.5
51	333464.0
52	333464.0
53	333464.0
54	315668.5
55	297873.0
56	297873.0
57	297873.0
58	276233.0
59	254593.0
60	254593.0
61	254593.0
62	224298.0
63	194003.0
64	194003.0
65	194003.0
66	158387.5
67	122772.0
68	122772.0
69	122772.0
70	93594.5
71	64417.0
72	64417.0
73	64417.0
74	48632.0
75	32847.0
76	32847.0
77	32847.0
78	25941.0
79	19035.0
80	19035.0
81	19035.0
82	13152.0
83	7269.0
84	7269.0
85	7269.0
86	5290.0
87	3311.0
88	3311.0
89	3311.0
90	2213.0
91	1115.0
92	1115.0
93	1115.0
94	695.0
95	275.0
96	275.0
97	275.0
98	615.0
99	955.0
100	955.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011631539417625437
2	2.907884854406359E-4
3	4.154121220580513E-4
4	0.009928349717187427
5	0.03435458249420085
6	0.06438887891899796
7	0.10950263537450233
8	0.11066578931626488
9	0.1197633147893362
10	0.13559051663974797
11	0.14265252271473483
12	0.13467660997122025
13	0.13039786511402232
14	0.11623231175184277
15	0.13542435179092474
16	0.12076030388227553
17	0.1261191202568244
18	0.11365675659508286
19	0.10057127475025422
20	0.09512937595129375
21	0.09508783473908795
22	0.10626242082244955
23	0.08304088319940446
24	0.09238765594571062
25	0.09093371351850743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2407248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.4750992337968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3061678021543	36.76305025694741
2	14.327141042363797	14.176734496151036
3	4.73993256260018	7.03525901688446
4	2.1299070094049317	4.215094425962734
5	1.1634690659626075	2.8781373746976433
6	0.7184510134391894	2.132726111071546
7	0.4699245615354747	1.627469502005624
8	0.3513796271874157	1.3907633539065534
9	0.24507991126017772	1.0912817636826664
>10	1.3809745452490276	12.339958339158812
>50	0.08987480908492772	3.068303719334647
>100	0.06343301808201315	6.515535437261183
>500	0.009453748803068821	3.1147572384618676
>1k	0.004811282872989957	3.6509289644737075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4232	0.17580241005496733	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2747	0.1141137099293467	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2440	0.10136055778216452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.308242441161027E-5
2	0.0	0.0	0.0	0.0	8.308242441161027E-5
3	0.0	0.0	0.0	0.0	8.308242441161027E-5
4	0.0	0.0	0.0	0.0	8.308242441161027E-5
5	0.0	0.0	0.0	0.0	8.308242441161027E-5
6	0.0	0.0	0.0	0.0	8.308242441161027E-5
7	0.0	0.0	0.0	0.0	8.308242441161027E-5
8	0.0	0.0	0.0	0.0	8.308242441161027E-5
9	0.0	0.0	0.0	0.0	8.308242441161027E-5
10	0.0	0.0	0.0	0.0	8.308242441161027E-5
11	0.0	0.0	0.0	0.0	8.308242441161027E-5
12	0.0	0.0	0.0	0.0	2.907884854406359E-4
13	0.0	0.0	0.0	0.0	3.323296976464411E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	215	0.0	14.142107	9
GGTATCA	1050	0.0	13.745441	1
GCGTTAT	345	0.0	13.210722	1
CGAACGA	110	2.752131E-9	12.953396	16
CGCGTAA	250	0.0	12.542541	10
GTATCAA	2680	0.0	12.223307	1
CGGTCCA	415	0.0	12.134989	10
GGGTATA	55	0.0030809084	12.084807	1
GTATAGA	185	0.0	11.804852	1
CGTTATT	355	0.0	11.771889	2
GCGTAAC	260	0.0	11.694677	11
ACTCTAA	375	0.0	11.40231	10
CGCATCG	365	0.0	11.193117	13
CGCGCTA	85	5.3302494E-5	11.175479	16
CGCATGT	60	0.005869295	11.085349	12
CGAGCCG	465	0.0	11.032198	15
GGTCGCG	305	0.0	10.903621	7
TAACGAA	140	8.341885E-9	10.8584385	13
AGAACCG	245	0.0	10.858213	5
CGCCAGT	395	0.0	10.820925	18
>>END_MODULE