FastQCFastQC Report
Thu 2 Feb 2017
SRR4062123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407248
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25080.10418536021215927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29200.027.5807461
GGTATCA12000.026.0384351
GTATAGG3050.018.036321
ACCGTCG6100.018.0318248
TCAACGC44700.017.5203194
ATCAACG45000.017.4035153
TACCGTC6600.017.3324077
AACGCAG46300.017.0574056
CAACGCA46200.016.9514775
ATACGAA5200.016.92463540
TATCAAC47550.016.7488372
CGCAATA5600.016.5015236
CAATACG5650.015.9657438
CGTCGTA7200.015.888370510
GTATTAT2500.015.8431041
GTTCTAG2950.015.6640851
GTATTAC2700.015.4845151
CCGTCGT7250.015.4750369
CGAATGC5550.015.46120543
ATACCGT7400.015.4586356
Produced by FastQC (version 0.11.5)