##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2407248
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27422185001296	32.0	32.0	32.0	32.0	32.0
2	31.431697523479095	32.0	32.0	32.0	32.0	32.0
3	31.558576847919284	32.0	32.0	32.0	32.0	32.0
4	31.638082158547853	32.0	32.0	32.0	32.0	32.0
5	31.601879822934738	32.0	32.0	32.0	32.0	32.0
6	35.25014788671545	36.0	36.0	36.0	36.0	36.0
7	35.25241354442916	36.0	36.0	36.0	36.0	36.0
8	35.201584132586255	36.0	36.0	36.0	36.0	36.0
9	35.29234628089835	36.0	36.0	36.0	36.0	36.0
10	35.16233557988209	36.0	36.0	36.0	36.0	36.0
11	35.289081557031096	36.0	36.0	36.0	36.0	36.0
12	35.20864489242488	36.0	36.0	36.0	36.0	36.0
13	35.24841561816647	36.0	36.0	36.0	36.0	36.0
14	35.206733581251285	36.0	36.0	36.0	36.0	36.0
15	35.18589900168159	36.0	36.0	36.0	36.0	36.0
16	35.19674291971579	36.0	36.0	36.0	36.0	36.0
17	35.17688331239656	36.0	36.0	36.0	36.0	36.0
18	35.17158514619183	36.0	36.0	36.0	36.0	36.0
19	35.16223297308794	36.0	36.0	36.0	36.0	36.0
20	35.16558451808871	36.0	36.0	36.0	36.0	36.0
21	35.160303383780985	36.0	36.0	36.0	36.0	36.0
22	35.14333629106764	36.0	36.0	36.0	36.0	36.0
23	35.11260451768991	36.0	36.0	36.0	36.0	36.0
24	35.093675848936215	36.0	36.0	36.0	36.0	36.0
25	35.07890670176068	36.0	36.0	36.0	36.0	36.0
26	35.033443168298405	36.0	36.0	36.0	36.0	36.0
27	35.02614022319263	36.0	36.0	36.0	36.0	36.0
28	35.005600170817466	36.0	36.0	36.0	36.0	36.0
29	34.98414164224043	36.0	36.0	36.0	36.0	36.0
30	34.96410423853296	36.0	36.0	36.0	36.0	36.0
31	34.96790276697706	36.0	36.0	36.0	36.0	36.0
32	34.94496121712429	36.0	36.0	36.0	36.0	36.0
33	34.92631378237722	36.0	36.0	36.0	32.0	36.0
34	34.91223961968189	36.0	36.0	36.0	32.0	36.0
35	34.902052468212666	36.0	36.0	36.0	32.0	36.0
36	34.879555409330486	36.0	36.0	36.0	32.0	36.0
37	34.870626956591096	36.0	36.0	36.0	32.0	36.0
38	34.85619387782231	36.0	36.0	36.0	32.0	36.0
39	34.84219448930895	36.0	36.0	36.0	32.0	36.0
40	34.827193957581436	36.0	36.0	36.0	32.0	36.0
41	34.803623473775865	36.0	36.0	36.0	32.0	36.0
42	34.76600250576592	36.0	36.0	36.0	32.0	36.0
43	34.765108538879254	36.0	36.0	36.0	32.0	36.0
44	34.71853668587532	36.0	36.0	36.0	32.0	36.0
45	34.70107732979734	36.0	36.0	36.0	32.0	36.0
46	34.6897901670289	36.0	36.0	36.0	32.0	36.0
47	34.667746738184015	36.0	36.0	36.0	32.0	36.0
48	34.63828280260281	36.0	36.0	36.0	32.0	36.0
49	34.62885253201997	36.0	36.0	36.0	32.0	36.0
50	34.20152057453158	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	26.0
21	56.0
22	186.0
23	492.0
24	1133.0
25	2705.0
26	5709.0
27	11102.0
28	18612.0
29	29614.0
30	44726.0
31	66547.0
32	101715.0
33	174211.0
34	400662.0
35	1549745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72841056462775	17.842654179681446	12.26867905586358	26.16025619982722
2	16.406543075245366	19.709653632445345	36.54621176714961	27.33759152515968
3	18.441550025381787	23.14320395407517	28.45701172879458	29.95823429174847
4	12.901880406073996	15.720703243457463	34.73720591912916	36.64021043133938
5	15.103234152222084	36.18915557446144	32.74538622143312	15.962224051883355
6	35.59331750333576	34.55274994973505	15.999416760411492	13.854515786517693
7	31.220588821758287	29.96371790525945	19.857696423467793	18.957996849514466
8	28.815061846556734	32.67203877622912	18.612124716688932	19.900774660525215
9	27.215102058450146	14.26994642845274	18.383731131981413	40.131220381115696
10	16.82806126666686	26.284631365206813	30.437051121052388	26.450256247073938
11	37.52459239762584	21.08382684293434	21.60537676217822	19.7862039972616
12	24.67635241570457	23.325099865074144	28.08476733597868	23.913780383242607
13	29.521303995267623	19.10006779525832	25.052674257076962	26.325953952397096
14	23.930147620851695	19.106631306786838	24.507155058390328	32.45606601397114
15	25.481628814314107	26.73201930170884	21.73938871275415	26.04696317122291
16	26.340839074386096	25.593422132713865	22.900320036689564	25.16541875621047
17	24.5541797106073	25.93014928250018	24.478159292270675	25.03751171462184
18	25.398598482000395	24.76052519745585	25.339485312475208	24.501391008068556
19	25.95656949346308	24.765167527400582	24.918745388925444	24.359517590210896
20	26.13112942623087	24.068684468519965	24.51458932221133	25.285596783037832
21	26.975658511295887	23.990673167035553	24.260732587585494	24.772935734083067
22	26.3574837324613	23.772124849620813	24.758105521325596	25.11228589659229
23	24.942880833217018	23.865384871022844	25.252695193847913	25.939039101912226
24	25.19879728783255	24.5158119761086	24.88366277200396	25.401727964054892
25	25.376540714834356	24.029688735204545	24.962000097206637	25.63177045275446
26	25.156673332674213	24.924540074558475	25.115879692826383	24.802906899940925
27	25.598517140577115	24.413384451328305	24.622212732335484	25.3658856757591
28	25.23100365443357	24.256931148178413	25.232914577978764	25.27915061940925
29	25.183072730596034	24.503290074940594	25.147430252072912	25.166206942390456
30	25.133743154984202	24.58053377622209	25.193023006564403	25.092700062229305
31	25.67932570080258	24.309125803825125	24.442847410312226	25.56870108506007
32	25.551328018517527	24.371591781933862	24.245181085503564	25.831899114045047
33	25.147004147895206	24.14070856933964	24.827177956543682	25.885109326221468
34	25.784937133162543	24.258120637363014	24.943887811348393	25.013054418126053
35	26.05496248403775	24.093796967650118	25.01136154985664	24.839878998455493
36	25.088046672421516	24.632796149624923	24.649703437039673	25.629453740913892
37	26.032534800704717	24.328402168986713	24.633278317984285	25.00578471232428
38	25.22012688347856	24.100092719985643	25.07772360803706	25.602056788498732
39	25.915699026932177	24.19392896073166	24.424691928322652	25.465680084013503
40	25.919610109988895	24.40638522990824	24.733855285901758	24.94014937420111
41	25.02318022751518	24.7783338816828	25.117064303114294	25.081421587687725
42	26.1027052020624	24.662093811523643	24.589018417574984	24.646182568838974
43	25.75698471511319	23.832882800358433	24.828832781020203	25.581299703508176
44	25.327491869045627	24.47254211292233	24.539342851884733	25.66062316614731
45	25.466124402311408	24.65804527270386	24.562456640315055	25.313373684669678
46	25.311928667360974	24.56030884001793	24.59412404697406	25.53363844564704
47	25.39852926148189	24.465422025185838	24.928652315985286	25.207396397346987
48	26.094687117415667	24.946731589943123	23.971926955913407	24.986654336727806
49	25.417910701978645	24.97124196093892	24.176859551511683	25.433987785570757
50	25.506375441071516	25.013438816619765	24.28865664176643	25.191529100542287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	341.0
1	224.5
2	108.0
3	254.5
4	401.0
5	419.0
6	437.0
7	337.0
8	237.0
9	348.5
10	460.0
11	773.0
12	1086.0
13	2037.0
14	2988.0
15	3795.5
16	4603.0
17	4434.0
18	4265.0
19	3975.0
20	3685.0
21	4426.5
22	5168.0
23	6267.5
24	7367.0
25	9532.5
26	11698.0
27	14683.5
28	17669.0
29	20638.5
30	23608.0
31	27064.0
32	30520.0
33	35852.5
34	41185.0
35	48315.5
36	55446.0
37	66763.0
38	78080.0
39	87207.5
40	96335.0
41	107594.5
42	118854.0
43	123951.0
44	129048.0
45	141413.5
46	153779.0
47	165598.0
48	177417.0
49	184848.5
50	192280.0
51	188802.0
52	185324.0
53	185513.5
54	185703.0
55	186119.5
56	186536.0
57	180754.5
58	174973.0
59	161136.0
60	147299.0
61	129446.0
62	111593.0
63	96378.0
64	81163.0
65	68341.5
66	55520.0
67	47811.5
68	40103.0
69	35442.5
70	30782.0
71	24282.5
72	17783.0
73	15624.5
74	13466.0
75	10037.0
76	6608.0
77	5917.0
78	5226.0
79	4407.0
80	3588.0
81	2825.5
82	2063.0
83	1680.0
84	1297.0
85	1007.5
86	718.0
87	471.0
88	224.0
89	152.0
90	80.0
91	60.0
92	40.0
93	32.0
94	24.0
95	24.0
96	24.0
97	30.0
98	36.0
99	23.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028621895209799738
2	0.005109569101314031
3	4.154121220580513E-4
4	8.308242441161027E-5
5	8.308242441161027E-5
6	1.6616484882322054E-4
7	0.0
8	0.0
9	0.0
10	4.1541212205805134E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.969890929393231E-4
17	0.0
18	4.1541212205805134E-5
19	0.0
20	3.323296976464411E-4
21	0.0
22	0.0
23	0.0
24	9.139066685277129E-4
25	2.0770606102902566E-4
26	4.154121220580513E-4
27	2.907884854406359E-4
28	0.0014539424272031797
29	3.323296976464411E-4
30	9.139066685277129E-4
31	3.323296976464411E-4
32	6.23118183087077E-4
33	1.246236366174154E-4
34	7.062006074986873E-4
35	2.492472732348308E-4
36	8.308242441161027E-5
37	0.0017031897004380107
38	0.0
39	6.646593952928821E-4
40	2.0770606102902566E-4
41	9.969890929393231E-4
42	0.0060234757698417445
43	0.004112580008374708
44	0.003697167886316657
45	0.002409390307936698
46	0.0019524369736728415
47	9.139066685277129E-4
48	0.004112580008374708
49	0.0039464151595514875
50	0.0017447309126438157
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2407248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.4340746740651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73961869947034	37.18989435926174
2	14.9226467695804	15.052197630234284
3	4.7021812970836745	7.1145048800433095
4	2.1368975253581493	4.310897974589513
5	1.1786128453724547	2.9721124127663363
6	0.726420092013362	2.1981795099205885
7	0.4806806953237361	1.6969880257637189
8	0.33720563022177485	1.3605323148096142
9	0.2649566312800671	1.2026558274630874
>10	1.3567348357384894	12.19912124709159
>50	0.0837288750354693	2.942025727981759
>100	0.058217901014155984	6.010450886087958
>500	0.008203575673291559	2.8268454849805837
>1k	0.0038946268347945286	2.9235937190059906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2508	0.10418536021215927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6616484882322054E-4
2	0.0	0.0	0.0	0.0	1.6616484882322054E-4
3	0.0	0.0	0.0	0.0	1.6616484882322054E-4
4	0.0	0.0	0.0	0.0	1.6616484882322054E-4
5	0.0	0.0	0.0	0.0	1.6616484882322054E-4
6	0.0	0.0	0.0	0.0	1.6616484882322054E-4
7	0.0	0.0	0.0	0.0	1.6616484882322054E-4
8	0.0	0.0	0.0	0.0	2.0770606102902568E-4
9	0.0	0.0	0.0	0.0	2.0770606102902568E-4
10	0.0	0.0	0.0	0.0	2.0770606102902568E-4
11	0.0	0.0	0.0	0.0	2.0770606102902568E-4
12	0.0	0.0	0.0	4.1541212205805134E-5	3.323296976464411E-4
13	0.0	0.0	0.0	4.1541212205805134E-5	4.5695333426385646E-4
14	0.0	0.0	0.0	4.1541212205805134E-5	4.984945464696617E-4
15	0.0	0.0	0.0	1.6616484882322054E-4	5.400357586754668E-4
16	0.0	0.0	0.0	2.0770606102902568E-4	6.23118183087077E-4
17	0.0	0.0	0.0	3.323296976464411E-4	6.23118183087077E-4
18	0.0	0.0	0.0	3.738709098522462E-4	6.646593952928821E-4
19	0.0	0.0	0.0	3.738709098522462E-4	6.646593952928821E-4
20	0.0	0.0	0.0	4.1541212205805137E-4	7.062006074986873E-4
21	0.0	0.0	0.0	5.815769708812719E-4	7.477418197044924E-4
22	0.0	0.0	0.0	7.477418197044924E-4	7.892830319102975E-4
23	0.0	0.0	0.0	9.969890929393233E-4	8.308242441161027E-4
24	0.0	0.0	0.0	0.0014539424272031797	8.308242441161027E-4
25	0.0	4.1541212205805134E-5	0.0	0.0020355193980844516	8.308242441161027E-4
26	0.0	4.1541212205805134E-5	0.0	0.002783261217788944	9.139066685277129E-4
27	0.0	4.1541212205805134E-5	0.0	0.003115590915435385	9.554478807335181E-4
28	0.0	4.1541212205805134E-5	0.0	0.004071038796168903	9.554478807335181E-4
29	0.0	4.1541212205805134E-5	0.0	0.005151110313519836	9.554478807335181E-4
30	0.0	4.1541212205805134E-5	0.0	0.007477418197044924	9.554478807335181E-4
31	0.0	4.1541212205805134E-5	0.0	0.011589998205419633	9.554478807335181E-4
32	0.0	4.1541212205805134E-5	0.0	0.016616484882322053	9.554478807335181E-4
33	0.0	4.1541212205805134E-5	0.0	0.021809136408047696	9.969890929393233E-4
34	0.0	4.1541212205805134E-5	0.0	0.027707988541272027	0.0010800715173509335
35	0.0	4.1541212205805134E-5	0.0	0.03402225279655441	0.0010800715173509335
36	0.0	4.1541212205805134E-5	0.0	0.04311977826962573	0.0010800715173509335
37	0.0	4.1541212205805134E-5	0.0	0.057534578905040115	0.0010800715173509335
38	0.0	4.1541212205805134E-5	0.0	0.07410952257515636	0.0011216127295567387
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2920	0.0	27.580746	1
GGTATCA	1200	0.0	26.038435	1
GTATAGG	305	0.0	18.03632	1
ACCGTCG	610	0.0	18.031824	8
TCAACGC	4470	0.0	17.520319	4
ATCAACG	4500	0.0	17.403515	3
TACCGTC	660	0.0	17.332407	7
AACGCAG	4630	0.0	17.057405	6
CAACGCA	4620	0.0	16.951477	5
ATACGAA	520	0.0	16.924635	40
TATCAAC	4755	0.0	16.748837	2
CGCAATA	560	0.0	16.50152	36
CAATACG	565	0.0	15.96574	38
CGTCGTA	720	0.0	15.8883705	10
GTATTAT	250	0.0	15.843104	1
GTTCTAG	295	0.0	15.664085	1
GTATTAC	270	0.0	15.484515	1
CCGTCGT	725	0.0	15.475036	9
CGAATGC	555	0.0	15.461205	43
ATACCGT	740	0.0	15.458635	6
>>END_MODULE